Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma

Ding, Xu; Yang, Wei; Hu, Jiajian; Shen, Yang; Han, Wei; Wang, Jing; Wang, Xu; Wang, Huanmin

doi:10.1186/s12974-020-01839-9

Cited by 8 publications

(10 citation statements)

References 57 publications

(136 reference statements)

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“…IL-6 is an important in ammatory cytokine that is mainly synthesized and secreted by astrocytes and microglia (Ding et al, 2020;Savarin et al, 2015). Laird et al reported that HMGB1 can trigger IL-6 release and that IL-6 enhances AQP4 expression and cellular swelling of brain astrocytes (Laird et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Tanshione IIA reduces AQP4 over-clustering in the plasma membrane and astrocyte swelling after oxygen-glucose deprivation and reoxygenation by inhibition of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis

Tang

Yang

Liao

et al. 2022

Preprint

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Background: Tanshione IIA (TSO IIA) has emerged as a powerful anti-inflammatory compound with multiple therapeutic properties. Here, we investigated the role of TSO IIA in astrocytic swelling caused by ischemia and reperfusion-like injury in an in vitro model and the molecular mechanisms underlying this effect. Methods: Primary brain astrocytes were cultured in vitro in conditions of glucose and oxygen deprivation and reoxygenation (OGD/R). We investigated the effects of TSO IIA treatment on cell swelling and injury, and the expression levels of AQP4 protein in the plasma membrane. We then studied the involvement of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis in TSO IIA-mediated protection. Results: Treatment with TSO IIA alleviated OGD/R-induced astrocytic swelling, changes in cell morphology and ultrastructure, the release of LDH, and the over-clustering of AQP4 protein in the plasma membrane. In addition, TSO-IIA significantly reduced over-expression of HMGB1 protein in the cytoplasm and surrounding medium, the high levels of NF-κB protein in the nucleus, and of IL-6 protein in cytoplasm and extracellular media induced by OGD/R. Combination of TSO IIA and recombinant HMGB1 (rHMGB1) reversed these effects. Inhibition of RAGE, the receptor of HMGB1, via administration of FPS-ZM1 (a RAGE antagonist) induced similar results to those of TSO IIA. In addition, exogenous IL-6 reversed TSO IIA-mediated effect on AQP4 protein clustering in the plasma membrane, cell swelling and LDH activity.Conclusions: TSO IIA significantly reduced astrocyte swelling after OGD/R injury in vitro, via blocking activation of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis and thereby decreasing the expression of AQP4 in plasma membrane.

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Section: Discussionmentioning

confidence: 99%

Tanshione IIA reduces AQP4 over-clustering in the plasma membrane and astrocyte swelling after oxygen-glucose deprivation and reoxygenation by inhibition of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis

Tang

Yang

Liao

et al. 2022

Preprint

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“…Moreover, the cGMP signaling pathway interrogated here is a putative key regulator of cell proliferation, differentiation, apoptosis, inflammation, and other processes ( 58 , 59 ). sGC is the primary sensor of nitric oxide (NO), binding of which boosts the enzymatic activity ( 60 – 64 ). Accumulated evidence have proved that NO is produced and binding to sGC in a variety of virulent microbial infections ( 65 – 68 ).…”

Section: Discussionmentioning

confidence: 99%

Virulence of Vibrio alginolyticus Accentuates Apoptosis and Immune Rigor in the Oyster Crassostrea hongkongensis

et al. 2021

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Vibrio species are ubiquitously distributed in marine environments, with important implications for emerging infectious diseases. However, relatively little is known about defensive strategies deployed by hosts against Vibrio pathogens of distinct virulence traits. Being an ecologically relevant host, the oyster Crassostrea hongkongensis can serve as an excellent model for elucidating mechanisms underlying host-Vibrio interactions. We generated a Vibrio alginolyticus mutant strain (V. alginolyticus△vscC) with attenuated virulence by knocking out the vscC encoding gene, a core component of type III secretion system (T3SS), which led to starkly reduced apoptotic rates in hemocyte hosts compared to the V. alginolyticusWT control. In comparative proteomics, it was revealed that distinct immune responses arose upon encounter with V. alginolyticus strains of different virulence. Quite strikingly, the peroxisomal and apoptotic pathways are activated by V. alginolyticusWT infection, whereas phagocytosis and cell adhesion were enhanced in V. alginolyticus△vscC infection. Results for functional studies further show that V. alginolyticusWT strain stimulated respiratory bursts to produce excess superoxide (O2•−) and hydrogen peroxide (H2O2) in oysters, which induced apoptosis regulated by p53 target protein (p53tp). Simultaneously, a drop in sGC content balanced off cGMP accumulation in hemocytes and repressed the occurrence of apoptosis to a certain extent during V. alginolyticus△vscC infection. We have thus provided the first direct evidence for a mechanistic link between virulence of Vibrio spp. and its immunomodulation effects on apoptosis in the oyster. Collectively, we conclude that adaptive responses in host defenses are partially determined by pathogen virulence, in order to safeguard efficiency and timeliness in bacterial clearance.

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“…This disease is characterized by the presence of rapid, irregular, horizontal and vertical eye movements, myoclonias that can affect the trunk, limbs or face and ataxia cerebellar. Most patients suffer from persistent deficits in cognition, neurology, and behavior [47,48]. Figure 8b shows an average Periodogram representing dolphin s brainwaves during DAT of Intervention Patient for one sample.…”

Section: Methodsmentioning

confidence: 99%

Non-Parametric Evaluation Methods of the Brain Activity of a Bottlenose Dolphin during an Assisted Therapy

Escobar

Matamoros

Villar

et al. 2021

Animals

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Dolphin-Assisted Therapies (DAT) are alternative therapies aimed to reduce anxiety levels, stress relief and physical benefits. This paper is focused on measuring and analyzing dolphins brain activity when DAT is taking place in order to identify if there is any differences in female dolphin’s neuronal signal when it is interacting with control or intervention subjects, performing our research in Delfiniti, Ixtapa, Mexico facilities. We designed a wireless and portable electroencephalographic single-channel signal capture sensor to acquire and monitor the brain activity of a female bottle-nose dolphin. This EEG sensor was able to show that dolphin activity at rest is characterized by high spectral power at slow-frequencies bands. When the dolphin participated in DAT, a 23.53% increment in the 12–30 Hz frequency band was observed, but this only occurred for patients with some disease or disorder, given that 0.5–4 Hz band keeps it at 17.91% when there is a control patient. Regarding the fractal or Self-Affine Analysis, we found for all samples studied that at the beginning the dolphin’s brain activity behaved as a self-affine fractal described by a power-law until the fluctuations of voltage reached the crossovers, and after the crossovers these fluctuations left this scaling behavior. Hence, our findings validate the hypothesis that the participation in a DAT of a Patient with a certain disease or disorder modifies the usual behavior of a female bottle-nose dolphin.

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Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma

Cited by 8 publications

References 57 publications

Tanshione IIA reduces AQP4 over-clustering in the plasma membrane and astrocyte swelling after oxygen-glucose deprivation and reoxygenation by inhibition of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis

Tanshione IIA reduces AQP4 over-clustering in the plasma membrane and astrocyte swelling after oxygen-glucose deprivation and reoxygenation by inhibition of the HMGB1/RAGE/NF-κB/IL-6 pro-inflammatory axis

Virulence of Vibrio alginolyticus Accentuates Apoptosis and Immune Rigor in the Oyster Crassostrea hongkongensis

Non-Parametric Evaluation Methods of the Brain Activity of a Bottlenose Dolphin during an Assisted Therapy

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