Serum-free modified trichosel medium for the isolation ofTrichomonas vaginalis

Smith, Rodney F.; Welch, R. P.; Stickney, Richard; Venerable, Sandra; Fredrickson, Sandra; Shima, Kathy; Hilton, Phyllis

doi:10.1007/bf01568966

Cited by 4 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The formulations of STS and Difco Kupferberg are the same, whereas Trichosel contains yeast extract, but we performed no experiments to determine whether addition of yeast extract to the other two media would improve their performance. Yeast extract has been found to enhance the growth of T. vaginalis in serum-free medium, although it appears to offer no advantage in media containing serum (6). Lash medium compared poorly with modified Diamond medium, and we suspect that it would have compared poorly with the Kupferberg media, had we performed this direct experiment.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions

et al. 1989

View full text Add to dashboard Cite

Many media have been formulated for the growth of Trichomonas vaginalis, but the relative sensitivities of these media have not been determined. We evaluated the ability of six media, including all five media commercially available in the United States, to grow Trichomonas vaginalis from vaginal secretions. In a first experiment, we evaluated the ability of five media to grow T. vaginalis from vaginal secretions of 375 women and determined the optimal days on which to read culture tubes, by inoculating aliquots of secretions into each medium and reading the tubes 1, 2, 3, 4, and 7 days later. Sixty-five patients (17%) had a positive wet-mount examination for T. vaginalis, and all the positive results were confirmed by growth in at least one medium. Of 310 wet-mount-negative specimens, 37 (12%) grew T. vaginalis; overall, 102 women (27%) had a positive culture. Diamond and modified Diamond media (the latter being the only medium not commercially available) detected 99 (97%) and 92 (90%) isolates, respectively, compared with three formulations of Kupferberg medium, which detected 77 (75%), 50 (49%), and 43 (42%) isolates. The optimal single day to read wet-mount-negative cultures was day 7, but 4 (11%) of the 37 positive specimens were positive only before day 7. In a second study, we compared the ability of modified Diamond medium with that of a sixth medium, Lash medium, to grow T. vaginalis from 48 wet-mount-positive specimens; modified Diamond medium supported growth in all cases, whereas Lash medium supported growth in only 26 (54%) cases. We conclude that formulations of Diamond medium are superior to formulations of Kupferberg or Lash medium for growth of t. vaginalis.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions

et al. 1989

View full text Add to dashboard Cite

show abstract

“…), 20.0 g; yeast extract (Difco Laboratories, Detroit, Mich.), 10.0 g; maltose, 5.0 g; ascorbic acid, 1.0 g; KCl, 1.0 g; KHCO3, 1.0 g; KH2PO4, 1.0 g; K2HPO4, 0.5 g; FeSO4, 0.1 g; agar, 1 g. The medium was designated Hollander fluid (HF) medium. Heat-inactivated pooled human serum (5%) was used in place of 10% horse serum, and 100 ,ug of chloramphenicol per ml replaced penicillin and streptomycin to prevent bacterial contaminants (16). The final medium had a pH of 6.0 and was dispensed in screw-cap tubes in 9.5-ml amounts.…”

Section: Methodsmentioning

confidence: 99%

Viability of Trichomonas vaginalis in vitro at four temperatures

Smith¹

1983

J Clin Microbiol

View full text Add to dashboard Cite

The effect of temperature as a possible factor on the survival of Trichomonas vaginalis for shipment or routine laboratory maintenance was studied. Ten strains of T. vaginalis, ATCC 30001, ATCC 30238, and eight clinical isolates, were examined for viability when kept incubated at 37°C or removed from this temperature and held at 42, 22, or 5°C for increasing lengths of time without subculture or reincubation at 37°C. The order in which the strains remained viable without subculture was: 5°C, 8 to 10 days; 22°C, 4 to 8 days; 37°C, 4 to 6 days; 42°C, less than 2 days. Vials of medium with cells were also held at 22 and 5°C and then reincubated at 37°C. Cultures held at 22°C remained viable 6 to 8 days, whereas those stored at 5°C remained viable 10 to 14 days. These data show that T. vaginalis withstands a wide range of temperatures, particularly below normal growth temperatures without subcultures, beyond what would be expected in mailing cultures.

show abstract

“…The isolation of Trichomonas vaginalis has been shown to be both more sensitive and specific than direct methods, including the wet mount, Papanicolaou smear, acridine orange, and numerous clinical parameters (2)(3)(4)(5)(6)(9)(10)(11). With the advent of monoclonal antibodies, highly sensitive and specific immunofluorescence assays are being developed for the direct detection of pathogens in clinical specimens.…”

mentioning

confidence: 99%