Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions

Schmid, George P.; Matheny, Linda C.; Zaidi, Akbar A.; Kraus, Stephen J.

doi:10.1128/jcm.27.6.1230-1233.1989

Cited by 40 publications

(27 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…23 The type of formulations to be used for cultivating T. vaginalis should also be taken into account. Schmid et al 5 determined that Diamond TYM medium proved to be superior to other media used for T. vaginalis cultivation. In this study, Diamond TYM medium was used for maintaining T. vaginalis isolates.…”

Section: Discussionmentioning

confidence: 99%

“…The culture method offers more sensitivity but with a longer turnaround period of at least 7 days to obtain the final result. 5 Current rapid methods in the specific detection of T. vaginalis are serologic-based but may exhibit cross-reaction with other trichomonads. 6 We have recently reported the prevalence of T. vaginalis in vaginal swabs from sex workers in Angeles City, Philippines using PCR utilizing T. vaginalis-specific primers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis and molecular characterization ofTrichomonas vaginalisin sex workers in the Philippines

Queza

Rivera

2013

Pathogens and Global Health

View full text Add to dashboard Cite

Trichomonas vaginalis is a pathogenic protozoon which causes the sexually transmitted infection, trichomoniasis. The absence or non-specificity of symptoms often leads to misdiagnosis of the infection. In this study, 969 samples consisting of vaginal swabs and urine were collected and screened from social hygiene clinics across the Philippines. Of the 969 samples, 216 were used for the comparative analysis of diagnostic tools such as wet mount microscopy, culture, and PCR utilizing universal trichomonad primers, TFR1/2 and species-specific primers, TVK3/7 and TV1/2. PCR demonstrated higher sensitivity of 100% compared to 77% of the wet mount. PCR primer set TVK3/7 and culture had the same and the best expected average performance [receiver-operating characteristic (ROC): 0·98]. Prevalence of infection in the sample population was 6·8%.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diagnosis and molecular characterization ofTrichomonas vaginalisin sex workers in the Philippines

Queza

Rivera

2013

Pathogens and Global Health

View full text Add to dashboard Cite

show abstract

“…Culture is considered the most reliable method of diagnosis but requires a special medium and frequent microscopic observation for up to 7 days (12,14,20). The sensitivity of culture is less than 90%, allowing for false negatives due to lack of detection of nonviable or small numbers of parasites (3,10,25). Cytology preparations, such as Papanicolaou's stain test (Pap smear) (10,19,29), also lack both sensitivity and specificity.…”

mentioning

confidence: 99%

18S Ribosomal DNA-Based PCR for Diagnosis of Trichomonas vaginalis

et al. 2000

View full text Add to dashboard Cite

Trichomonas vaginalis remains the most common sexually transmitted parasite in the world and is considered a major risk factor in the transmission of the human immunodeficiency virus. A PCR technique using primers targeting a specific region of the 18S rRNA gene of T. vaginalis was developed. The PCR test was standardized using 15 reference strains, giving a single product of 312 bp in all strains. No amplification was observed when DNA from related organisms or human DNA was used as a target. The test was evaluated on 372 vaginal swab specimens and 361 urine samples from women attending infertility and obstetric clinics at two separate hospitals in Lima, Peru. Compared to T. vaginalis culture, the overall sensitivity and specificity of PCR of vaginal swab samples was 100% and 98%, respectively. The PCR of urine samples was 100% sensitive and 99.7% specific compared to culture of vaginal swab, but the sensitivity drops to 83.3% when compared to PCR of vaginal swabs. All culture-positive samples were found to be positive by PCR in either urine or vaginal secretion. None of the PCR-negative samples were positive by culture. The origin of the amplification was confirmed by digestion of PCR products with HaeIII. This PCR assay, which is easy to perform and has a high sensitivity and specificity, should be useful for routine diagnosis of T. vaginalisinfection.

show abstract

“…When two media were used concurrently for diagnosis by of T. vaginalis is the most sensitive the culture method, Krieger et al (11) reported a 7% trichomoniasis (2,8,10,11,13,15,17), increase in sensitivity compared with that when a single nce has prevented it from being widely culture was used. Similarly, when the results obtained by linical laboratory procedure.…”

Section: Resultsmentioning

confidence: 99%

“…In the diagnosis of Trichomonas vaginalis, culture of the organism has been shown to be more sensitive than immediate examination of glass slide wet mount preparations (2,8,10,11,13,15,17). Several investigators reported superior results (2, 10, 11, 15) when both methods were used to make a diagnosis.…”

mentioning

confidence: 99%

The plastic envelope method, a simplified technique for culture diagnosis of trichomoniasis

Beal¹,

Goldsmith²,

Kotby³

et al. 1992

J Clin Microbiol

View full text Add to dashboard Cite

Although culture of Trichomonas vaginalis is more sensitive than wet mounts in the diagnosis of trichomoniasis, the lack of convenience of culture prevents it from being widely used. To improve the acceptability of diagnosis by culture, a plastic envelope method (PEM) was devised. PEM permits both immediate examination and culture in one self-contained system. The medium consists of dry ingredients that are reconstituted with water before use. The effectiveness of immediate examinations by PEM was compared with that of wet mounts, and the effectiveness of culture by PEM was compared with that of culture in Trichomonas Medium No. 2 (Oxoid). Of 710 vaginal secretion specimens from symptomatic and asymptomatic women that were tested by the four methods, 62 (9%o) were positive for T. vaginalis. The sensitivity was 66% by wet mount, 66% by immediate examination by PEM, 89% by cultures in Oxoid medium, and 97% by culture by PEM. The two culture methods had equivalent sensitivities but were significantly (P < 0.0001) more sensitive than the two immediate methods. The combined immediate examination by PEM plus culture was more convenient to use than wet mounts plus culture in Oxoid medium. The long shelf-life of PEM's dry medium and its anticipated low cost are additional advantages.

show abstract

Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions

Cited by 40 publications

References 9 publications

Diagnosis and molecular characterization ofTrichomonas vaginalisin sex workers in the Philippines

Diagnosis and molecular characterization ofTrichomonas vaginalisin sex workers in the Philippines

18S Ribosomal DNA-Based PCR for Diagnosis of Trichomonas vaginalis

The plastic envelope method, a simplified technique for culture diagnosis of trichomoniasis

Contact Info

Product

Resources

About