Serum‐Free Media

Freshney, R. Ian

doi:10.1002/0471747599.cac010

Cited by 9 publications

(6 citation statements)

References 67 publications

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“…However, the serumfree media are difficult to design: only specific cell types have been cultivated this way to date. 3 At present, formulations of popular media for cell culture are available commercially. Thus, there are investigators who are using culture media without understanding their details and background, particularly regarding the rationale for their development, the exact ingredients, as well as the cell types that these media are suitable for.…”

mentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

377

278

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

377

278

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“…Nevertheless, in the last six decades researchers have been trying to alternate FBS with different sources include hormones [24], pituitary extracts, chick embryo extracts, bovine milk fractions, bovine colostrum or platelet lysates [14], bovine ocular fluid [25], fish serum [26], plant origin [27] and sericin protein [28], other protein fractions from plant extracts, called vegetal serum [29] and earthworm coelomic fluid [30]. However, the serum-free chemically defined media is the good one for clinical and pharmaceutical productions since it is specific to every cell type [31]. The clinical and pharmaceutical products also carried out in the specific cell line needs; therefore, they can be used based on chemically defined media suitable for them.…”

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confidence: 99%

Alternative to FBS in animal cell culture - An overview and future perspective

Chelladurai

Christyraj

Rajagopalan

et al. 2021

Heliyon

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Fetal bovine serum (FBS) is a widely used growth supplement in the in vitro culturing of animal and human cells, tissues and organs, notably due to the occurrence of abundant micro-and macronutrients, along with growth factors. Over the years, increasing demand, high price, batch-to-batch variability in quality and composition, increasing ethical concerns lead to the search for an alternative to FBS. Several approaches have been suggested and employed in the past, but none is implemented as widely as FBS, and each supplement has its own disadvantages. In this review, we described the importance of FBS in cell culture, discussed the issues associated with FBS use and presented the efforts made in the recent past to reduce or replace FBS. The potential of four different alternative sources to FBS, namely, bovine ocular fluid, sericin protein, human platelet lysate and earthworm heat inactivated coelomic fluid was evaluated. In the end, we present the conceptual perspective using the Human Platelet Lysate (HPL) and earthworm Heat Inactivated Coelomic Fluid (HI-CF) combination to alternate FBS and its context in scientific and economic impacts.

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“…Today a modified version of MEM known as Dulbecco’s modified MEM (DMEM) is still widely used. Serum from bovine, horses, and specifically fetal calves is often supplemented to improve culture growth and longevity by providing growth factors, hormones, vitamins, trace elements, and more . More recently, efforts have been made to use serum-free culture media, replacing components of biological origin with known factors .…”

Section: An Overview Of Neuronal Model Systemsmentioning

confidence: 99%

From Cultured Rodent Neurons to Human Brain Tissue: Model Systems for Pharmacological and Translational Neuroscience

Wellbourne-Wood

Chatton

2018

ACS Chem. Neurosci.

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To investigate the enormous complexity of the functional and pathological brain there are a number of possible experimental model systems to choose from. Depending on the research question choosing the appropriate model may not be a trivial task, and given the dynamic and intricate nature of an intact living brain several models might be needed to properly address certain questions. In this review, we aim to provide an overview of neural cell and tissue culture, reflecting on historic methodological milestones and providing a brief overview of the state-of-the-art. We additionally present an example of an effective model system pipeline, composed of dissociated mouse cultures, organotypics, acute mouse brain slices, and acute human brain slices, in that order. The sequential use of these four model systems allows a balance and progression from experimental control to human applicability, and provides a meta-model that can help validate basic research findings in a translational setting. We then conclude with a few remarks regarding the necessity of an integrated approach when performing translational and neuropharmacological studies.

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Serum‐Free Media

Cited by 9 publications

References 67 publications

Animal‐cell culture media: History, characteristics, and current issues

Animal‐cell culture media: History, characteristics, and current issues

Alternative to FBS in animal cell culture - An overview and future perspective

From Cultured Rodent Neurons to Human Brain Tissue: Model Systems for Pharmacological and Translational Neuroscience

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