Alternative to FBS in animal cell culture - An overview and future perspective

Chelladurai, Karthikeyan Subbiahanadar; Christyraj, Jackson Durairaj Selvan; Rajagopalan, Kamarajan; Yesudhason, Beryl Vedha; Venkatachalam, Saravanakumar; Mohan, Manikandan; Vasantha, Niranjan Chellathurai; Christyraj, Johnson Retnaraj Samuel Selvan

doi:10.1016/j.heliyon.2021.e07686

Cited by 79 publications

(56 citation statements)

References 107 publications

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“…Figure 5C shows the increase in cell number and metabolism over time in cells seeded with culture media 2% FBS. On the contrary, the cells seeded with culture media without FBS show a stationary behaviour in their metabolism and viability over the 5 h. These results are in agreement with the fact that FBS supplementation improve cell viability and metabolism ( Subbiahanadar Chelladurai et al, 2021 ). Furthermore, the plasmonic biosensor can accurately detect the cell adhesion and confluency by monitoring the free gold surface available.…”

Section: Resultssupporting

confidence: 84%

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Vila

Castro-Aguirre

López‐Muñoz

et al. 2021

Front. Bioeng. Biotechnol.

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Over the last years, optical biosensors based on plasmonic nanomaterials have gained great scientific interest due to their unquestionable advantages compared to other biosensing technologies. They can achieve sensitive, direct, and label-free analysis with exceptional potential for multiplexing and miniaturization. Recently, it has been demonstrated the potential of using optical discs as high throughput nanotemplates for the development of plasmonic biosensors in a cost-effective way. This work is a pilot study focused on the development of an integrated plasmonic biosensor for the monitoring of cell adhesion and growth of human retinal pigmented cell line (ARPE-19) under different media conditions (0 and 2% of FBS). We observed an increase of the plasmonic band displacement under 2% FBS compared to 0% conditions over time (1, 3, and 5 h). These preliminary results show that the proposed plasmonic biosensing approach is a direct, non-destructive, and real-time tool that could be employed in the study of living cells behavior and culture conditions. Furthermore, this setup could assess the viability of the cells and their growth over time with low variability between the technical replicates improving the experimental replicability.

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Section: Resultssupporting

confidence: 84%

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Vila

Castro-Aguirre

López‐Muñoz

et al. 2021

Front. Bioeng. Biotechnol.

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“…Fetal bovine serum (FBS) is often used in research in a broad range of cell cultures as a source of nutrients and growth factors. However, it has several issues: firstly, FBS does not simulate the human microenvironment, which limits its translational application ( 131 ); secondly, it involves the risk of transmitting bovine spongiform encephalopathy and some viral infections; thirdly, it can promote the development of unwanted immunological reactions; and, finally, variations between the brands and batches of sera can affect the reproducibility of the experiments ( 131 , 133 , 134 ). Human serum (HS) does not contain any xenogeneic components and supplements the medium with trophogens and additional stimuli that favor cell growth and survival.…”

Section: Strategies To Improve the Persistence Of Car-t Cellsmentioning

confidence: 99%

CAR-T Cell Performance: How to Improve Their Persistence?

López-Cantillo¹,

Urueña

Camacho³

et al. 2022

Front. Immunol.

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Adoptive cell therapy with T cells reprogrammed to express chimeric antigen receptors (CAR-T cells) has been highly successful in patients with hematological neoplasms. However, its therapeutic benefits have been limited in solid tumor cases. Even those patients who respond to this immunotherapy remain at risk of relapse due to the short-term persistence or non-expansion of CAR-T cells; moreover, the hostile tumor microenvironment (TME) leads to the dysfunction of these cells after reinfusion. Some research has shown that, in adoptive T-cell therapies, the presence of less differentiated T-cell subsets within the infusion product is associated with better clinical outcomes. Naive and memory T cells persist longer and exhibit greater antitumor activity than effector T cells. Therefore, new methods are being studied to overcome the limitations of this therapy to generate CAR-T cells with these ideal phenotypes. In this paper, we review the characteristics of T-cell subsets and their implications in the clinical outcomes of adoptive therapy with CAR-T cells. In addition, we describe some strategies developed to overcome the reduced persistence of CAR T-cells and alternatives to improve this therapy by increasing the expansion ability and longevity of modified T cells. These methods include cell culture optimization, incorporating homeostatic cytokines during the expansion phase of manufacturing, modulation of CAR-T cell metabolism, manipulating signaling pathways involved in T-cell differentiation, and strategies related to CAR construct designs.

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“… Averages and range estimates of concentrations given when available. *Note that the exact composition of BS is unknown, the above table provides only estimates of concentrations and constituents 40 , 41 . …”

Section: Methodsmentioning

confidence: 99%

“…*Note that the exact composition of BS is unknown, the above table provides only estimates of concentrations and constituents 40 , 41 .…”

Section: Methodsmentioning

confidence: 99%

Driving electrochemical corrosion of implanted CoCrMo metal via oscillatory electric fields without mechanical wear

Welles

Ahn

2021

Sci Rep

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Decades of research have been dedicated to understanding the corrosion mechanisms of metal based implanted prosthetics utilized in modern surgical procedures. Focused primarily on mechanically driven wear, current fretting and crevice corrosion investigations have yet to precisely replicate the complex chemical composition of corrosion products recovered from patients’ periprosthetic tissue. This work specifically targets the creation of corrosion products at the metal on metal junction utilized in modular hip prosthetics. Moreover, this manuscript serves as an initial investigation into the potential interaction between implanted CoCrMo metal alloy and low amplitude electrical oscillation, similar in magnitude to those which may develop from ambient electromagnetic radiation. It is believed that introduction of such an electrical oscillation may be able to initiate electrochemical reactions between the metal and surrounding fluid, forming the precursor to secondary wear particles, without mechanically eroding the metal’s natural passivation layer. Here, we show that a low magnitude electrical oscillation (≤ 200 mV) in the megahertz frequency (106 Hz) range is capable of initiating corrosion on implanted CoCrMo without the addition of mechanical wear. Specifically, a 50 MHz, 200 mVpp sine wave generates corrosion products comprising of Cr, P, Ca, O, and C, which is consistent with previous literature on the analysis of failed hip prosthetics. These findings demonstrate that mechanical wear may not be required to initiate the production of chemically complex corrosion products.

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Alternative to FBS in animal cell culture - An overview and future perspective

Cited by 79 publications

References 107 publications

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring

CAR-T Cell Performance: How to Improve Their Persistence?

Driving electrochemical corrosion of implanted CoCrMo metal via oscillatory electric fields without mechanical wear

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