Serum Free Hemoglobin Concentrations in Healthy Individuals Are Related to Haptoglobin Type

Na, Na; Ouyang, Jin; Taes, Youri; Delanghe, Joris

doi:10.1373/clinchem.2005.055657

Cited by 40 publications

(34 citation statements)

References 9 publications

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“…Plasma concentrations for cellular proteins can be elevated when there is nonphysiological breakage of blood cells during sample collection and preparation. The sum of the estimated concentrations for hemoglobin-␣ and -␤, 71 g/ml, is close to the 100 -200 g/ml measured in serum in (51), suggesting that such breakage was minimal.…”

Section: Choice Of Atlas Stringency Level For Analysis)supporting

confidence: 57%

A High-Confidence Human Plasma Proteome Reference Set with Estimated Concentrations in PeptideAtlas

Farrah

Deutsch

Omenn

et al. 2011

Molecular & Cellular Proteomics

387

494

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Human blood plasma can be obtained relatively noninvasively and contains proteins from most, if not all, tissues of the body. Therefore, an extensive, quantitative catalog of plasma proteins is an important starting point for the discovery of disease biomarkers. In 2005, we showed that different proteomics measurements using different sample preparation and analysis techniques identify significantly different sets of proteins, and that a comprehensive plasma proteome can be compiled only by combining data from many different experiments. Applying advanced computational methods developed for the analysis and integration of very large and diverse data sets generated by tandem MS measurements of tryptic peptides, we have now compiled a high-confidence human plasma proteome reference set with well over twice the identified proteins of previous high-confidence sets. It includes a hierarchy of protein identifications at different levels of redundancy following a clearly defined scheme, which we propose as a standard that can be applied to any proteomics data set to facilitate cross-proteome analyses. Further, to aid in development of blood-based diagnostics using techniques such as selected reaction monitoring, we provide a rough estimate of protein concentrations using spectral counting. We identified 20,433 distinct peptides, from which we inferred a highly nonredundant set of 1929 protein sequences at a false discovery rate of 1%. We have made this resource available via PeptideAtlas, a large, multiorganism, publicly accessible compendium of peptides identified in tandem MS experiments conducted by laboratories around the world. Molecular & Cellular

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Section: Choice Of Atlas Stringency Level For Analysis)supporting

confidence: 57%

A High-Confidence Human Plasma Proteome Reference Set with Estimated Concentrations in PeptideAtlas

Farrah

Deutsch

Omenn

et al. 2011

Molecular & Cellular Proteomics

387

494

View full text Add to dashboard Cite

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“…Serum free Hb has been suggested to be associated with stroke and atherosclerosis in some studies [8,[25][26][27][28]. Once released from red blood cells, Hb may become highly toxic due to the oxidative properties of its breakdown product heme [8] and activate endothelial cells resulting in inflammation and coagulation [27].…”

Section: Discussionmentioning

confidence: 99%

Serum free hemoglobin as a novel potential biomarker for acute ischemic stroke

Huang

Chen

et al. 2009

J Neurol

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“…Serum Hp levels have been reported to be lower in the subjects of Hp 2-2 than in Hp 1-1 and Hp 2-1 (2,7,26). In particular, the Hp 2 /Hp del genotype was reported to be associated with an abnormally low Hp concentration (3,6,7).…”

Section: Discussionmentioning

confidence: 99%