Serum d(-)-Lactate Levels as a Predictor of Acute Intestinal Ischemia in a Rat Model

Murray, Michael J.; Barbose, Jeffrey J.; Cobb, Charles F.

doi:10.1006/jsre.1993.1078

Cited by 101 publications

(62 citation statements)

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“…Most of these studies have focused on potential biological markers of gut ischemia and injury, including measuring various intracellular enzymes released by the ischemic bowel, such as aminotransferase, LDH, D(-)-lactate, tyrosin, creatine phosphokinase and alcohol dehydrogenase (10)(11)(12)(13)(14). Unfortunately, all these markers have a low specificity and can not be used in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Analysis of serum enzyme levels in a rabbit model of acute mesenteric ischemia

Zhang

2011

Mol Med Report

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Abstract. Early diagnosis of acute mesenteric ischemia (AMI) remains very difficult, partly due to the fact that useful markers of early small bowel ischemia have not yet been identified. Thus, in this study, we aimed to evaluate the levels of serum intracellular enzymes in the tissues and organs in a controlled animal model of mesenteric intestinal ischemia. Forty-eight New Zealand rabbits were divided into 4 groups including the control, artery ligation, vein ligation, and both artery and vein ligation groups. Plasma samples were obtained at 0-, 1-, 3-, 6-and 9-h time-points and enzyme levels were determined. The bowel color and vitality were observed at the same time. The bowel showed an appearance of infarction after a period of ischemia in the animals. Six and 9 h after superior mesenteric artery ischemia, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased significantly. However, after 3 h of superior mesenteric venous (SMV) ligation, γ-glutamyl transpeptidase levels in the blood were considerably higher compared to the control group. Six hours after SMV ischemia, ALT, AST, alkaline phosphatase and lactate dehydrogenase levels were significantly elevated compared to those pre-ligation. Serum enzyme levels during intestinal ischemia are not able to provide sufficient information as regards the extent and reversibility of intestinal ischemia, although, they may be able to reflect the presence of injury.

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Section: Discussionmentioning

confidence: 99%

Analysis of serum enzyme levels in a rabbit model of acute mesenteric ischemia

Zhang

2011

Mol Med Report

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“…18,19 In experimental studies, lactate concentration was found to increase rapidly after onset of intestinal ischemia. 20,21 Increased urinary D-lactate excretion in neonatal NEC 10 may, therefore, possibly be due to intestinal ischemia. Blood lactate changes have previously been reported in only one study of 24 patients with NEC, and the changes only showed a nonsignificant trend toward higher lactate concentrations in non-survivors.…”

Section: Discussionmentioning

confidence: 99%

A simple presurgical necrotizing enterocolitis-mortality scoring system

et al. 2006

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Objective: To assess the relationship between early laboratory parameters, disease severity, type of management (surgical or conservative) and outcome in necrotizing enterocolitis (NEC). Study design:Retrospective collection and analysis of data from infants treated in a single tertiary care center (1980 to 2002). Data were collected on disease severity (Bell stage), birth weight (BW), gestational age (GA) and pre-intervention laboratory parameters (leukocyte and platelet counts, hemoglobin, lactate, C-reactive protein).Results: Data from 128 infants were sufficient for analysis. Factors significantly associated with survival were Bell stage (P<0.05), lactate (P<0.05), BW and GA (P<0.01, P<0.001, respectively). From receiver operating characteristics curves, the highest predictive value resulted from a score with 0 to 8 points combining BW, Bell stage, lactate and platelet count (P<0.001). At a cutoff level of 4.5 sensitivity and specificity for predicting survival were 0.71 and 0.72, respectively.Conclusion: Some single parameters were associated with poor outcome in NEC. Optimal risk stratification was achieved by combining several parameters in a score.

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“…Therefore, intestinal barrier function injury after severe trauma could result in bacterial/toxin translocation, in turn evoke systemic inflammatory response syndrome and multiple organ dysfunction syndrome [3,[28][29] . The intestinal blood flow might keep relatively low despite of systemic circulation recovery during trauma, which was evident by significant decrease in intestinal mucosal pHi [30][31][32][33] . This may be the pathological basis for intestinal origin sepsis and multiple organ dysfunction syndrome.…”

Section: Discussionmentioning

confidence: 99%

Preventive effect of glutamine on intestinal barrier dysfunction induced by severe trauma

Li¹,

Lü²,

Hu³

et al. 2002

WJG

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AIM: To investigate the mechanism underlying intestinal barrier function damage after severe trauma and the therapeutic effect of glutamine. METHODS:Burned patients, and animal models of severe trauma replicated by hemorrhagic shock combined with endotoxin infusion and burn injury, were included in a serial experiment. Effects of oral glutamine on intestinal barrier function were observed in scalded rats. Parameters measured in these experiments were as follows: plasma levels of diamine oxidase (DAO), tumor necrosis factor (TNFα α α α α), endotoxin (LPS), and lactate as well as D-lactate by biochemical methods, lactose/mannitol (L/M) ratio in urine by SP-3400, and pathological examination of intestinal mucosa under light microscopy. RESULTS: INTRODCTIONIt is generally accepted that the intestine may serve as an important organ in the development of severe complications under critically ill conditions, including trauma, burns, shock, etc.[1-3] Hemorrhagic shock and/or gut ischemia-reperfusion injury commonly occur in the early stage after acute insults, leading to gut-derived sepsis as a result of gut barrier dysfunction [4][5][6][7][8][9][10] . In order to investigate the mechanism underlying intestinal barrier function damage and its potential interventional measures, burned patients and animal models of severe trauma were employed in our current experiments [6,[11][12][13][14][15] . MATERIALS AND METHODS Animal modelsAnimal models of severe trauma were replicated by hemorrhagic shock combined with endotoxin infusion. Male Wistar rats, weighing 190g-230g, were anaesthetized with intraperitoneal injection of 30g·L -1 barbitone sodium (35mg·kg -1 ), and the femoral artery and jugular vein were cannulated under aseptic conditions. The rats were then bled via the jugular vein catheter until a mean arterial pressure of 30-35 mmHg (4.6 kPa) was reached. At the end of shock, endotoxin (E.coliO55 B5, Sigma) was infused through tail vein at a dose of 2mg·kg -1 . A goat model of hemorrhagic shock combined with endotoxin challenge was established according to the previous report(n=20) [5] . Animals received E.coliO26 B6 endotoxin via portal vein 24h after the recovery from shock, and the dosage was 30 ng·kg -1 ·min -1, which was given in a continuous infusion lashing for 5d. Wistar rats were divided randomly into three groups: normal controls, early feeding with standard feed plase Gln 0.5g after scalding, and animals (except control group) sustained a 30% TBSA fullthickness scald covering the back and flanks [6] . Determination of plasma diamine oxidase in 21 burned patients (17 male and 4 female) at the age of 33±10 years, with burn area (64±21)%, and (35±20)% 0. Plasma DAO activity was determined on day 1, 3, 7, 14 and 21 postburn.Blood and intestinal DAO levels were tested according to our previous report [17] . Plasma lactate and Dlactate concentrations were determined by biochemical methods as described by Brandt et al [18] . Microassay for quantitation of endotoxin in blood was made with new PCA treatment...

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Serum d(-)-Lactate Levels as a Predictor of Acute Intestinal Ischemia in a Rat Model

Cited by 101 publications

References 0 publications

Analysis of serum enzyme levels in a rabbit model of acute mesenteric ischemia

Analysis of serum enzyme levels in a rabbit model of acute mesenteric ischemia

A simple presurgical necrotizing enterocolitis-mortality scoring system

Preventive effect of glutamine on intestinal barrier dysfunction induced by severe trauma

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