Analysis of serum enzyme levels in a rabbit model of acute mesenteric ischemia

Zhang, Fuxian

doi:10.3892/mmr.2011.553

Cited by 10 publications

(10 citation statements)

References 12 publications

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“…However, the specificity of aspartate aminotransferase as a serum marker for intestinal ischemia is weak. 24 The findings support the impression that postoperative HA and PV occlusions are difficult to detect with current standards of monitoring. 1 In contrast to this, we have shown that continuous intraorgan monitoring using hepatic and intra-abdominal PCO 2 measurements provides both real-time ischemia detection and identification of the affected vascular territory.…”

Section: Discussionsupporting

confidence: 66%

Hepatic and Abdominal Carbon Dioxide Measurements Detect and Distinguish Hepatic Artery Occlusion and Portal Vein Occlusion in Pigs

Pischke

Tronstad

Holhjem³

et al. 2012

Liver Transpl

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Hepatic artery (HA) occlusion and portal vein (PV) occlusion are the most common vascular complications after liver transplantation with an impact on mortality and retransplantation rates. The detection of severe hypoperfusion may be delayed with currently available diagnostic tools. Hypoperfusion and anaerobically produced lactic acid lead to increases in tissue carbon dioxide. We investigated whether the continuous assessment of the intrahepatic and intra-abdominal partial pressure of carbon dioxide (PCO 2 ) could be used to detect and distinguish HA and PV occlusions in real time. In 13 pigs, the HA and the PV were fully occluded (n ¼ 7) or gradually occluded (n ¼ 6). PCO 2 was monitored intrahepatically and between loops of small intestine. The hepatic and intestinal metabolism was assessed with microdialysis and PV as well as hepatic vein blood samples, and the results were compared to clinical parameters for the systemic circulation and blood gas analysis. Total HA occlusion led to significant increases in hepatic PCO 2 and lactate, and this was accompanied by significant decreases in the partial pressure of oxygen and glucose. PV occlusion induced a significant increase in intestinal PCO 2 (but not hepatic PCO 2 ) along with significant increases in intestinal lactate and glycerol. Gradual HA occlusion and PV occlusion caused steady hepatic and intestinal PCO 2 increases, respectively. Systemic clinical parameters such as the blood pressure, heart rate, and cardiac output were affected only by PV occlusion. In conclusion, even gradual HA occlusion affects liver metabolism and can be reliably identified with hepatic PCO 2 measurements. Intestinal PCO 2 increases only during PV occlusion. A combination of hepatic and intestinal PCO 2 measurements can reliably diagnose the affected vessel and depict the severity of the occlusion, and this may emerge as a potential real-time clinical monitoring tool for the postoperative course of liver transplantation and enable early interventions.

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Section: Discussionsupporting

confidence: 66%

Hepatic and Abdominal Carbon Dioxide Measurements Detect and Distinguish Hepatic Artery Occlusion and Portal Vein Occlusion in Pigs

Pischke

Tronstad

Holhjem³

et al. 2012

Liver Transpl

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“…20 In present study for elevated levels of alkaline phosphates sensitivity and specificity were 72% and 68% respectively whereas others reported similar sensitivity of up to 70% to 80% but slight higher specificity of 70% to 80% in their study. 10,24,25 They stated level >140 U was specific for bowel ischemia, which was found to be similar to present study. Sensitivity and specificity for elevated level of CPK-MB was found to be 76% and 84% respectively similar to the other studies which reported similar sensitivity of up to 70% to 80% and specificity of 80% to 90%.…”

Section: Discussionsupporting

confidence: 90%

Role of serum lactic dehydrogenase, glutamic oxaloacetic transaminase, creatine phosphokinase, alkaline phospatase, serum phosphorus in the cases of bowel ischemia in acute abdomen

et al. 2017

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Background: Bowel ischemia is a life-threatening condition which may arise from a number of causes affecting the arterial and venous compartments of the mesenteric circulation. The rapid onset of acute mesenteric ischemia and the potential rapidity with which bowel infarction may occur explain the lethality of this disease. The aim of this study was to evaluate the role of serum lactic dehydrogenase (LDH), glutamic oxaloacetic transaminase (SGOT), creatine phosphokinase (CPK), alkaline phospatase, serum phosphorus in the cases of bowel ischemia in acute abdomen.Methods: Above mentioned serum enzymes were measured preoperatively in the fifty patients of acute abdomen. Biomarker levels were compared in patients between equal two groups on the basis intra operative findings of bowel ischemia and non-bowel ischemia.Results: Preoperative estimated serum levels of all described enzymes found to be significantly elevated in patients of group A (with bowel ischemia) in comparison with group B (with no bowel ischemia). Sensitivity and specificity for elevated levels of S. LDH were 88% and 68%, for SGOT 68% and 80%, for S. ALP 72% and 68%, for S. CPK-MB 76% and 84%, and for S. phosphorus 76% and 68% respectively.Conclusions: Preoperative estimated serum levels of all described enzymes found to be significantly elevated in patients of group A (with bowel ischemia) in comparison with group B (with no bowel ischemia). Sensitivity and specificity for elevated levels of S. LDH were 88% and 68%, for SGOT 68% and 80%, for S. ALP 72% and 68%, for S. CPK-MB 76% and 84%, and for S. phosphorus 76% and 68% respectively.

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“…For this reason, many tests were investigated for the diagnosis of the AMI. Some of the parameters are white blood cell count in blood, AST, ALT, LDH, ALP, CRP, CK-MB, D-dimer, troponin I, α-glutathione S -transferase, lactate, procalcitonin, and phosphorus levels in plasma (6–10, 28). Presently, angiography appears to have the highest sensitivity.…”

Section: Discussionmentioning

confidence: 99%

A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia

Çakmaz¹,

Büyükaşık

Kahramansoy

et al. 2013

Libyan Journal of Medicine

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IntroductionThere is no valid and reliable diagnostic test for early diagnosis of acute mesenteric ischemia (AMI). The aim of this study was to measure the plasma levels of diamine oxidase (DAO) and citrulline in AMI to gain insight into its early diagnosis.Material and methodsA total of 21 Wistar albino rats were divided into three groups, that is, control group, short-term ischemia group, and prolonged ischemia group. The superior mesenteric artery was occluded for 15 min in the short-term ischemia group and for 12 h in the prolonged ischemia group. Twelve hours later, the experiment was terminated and plasma DAO and citrulline levels were measured. Intestinal tissue was evaluated for the histopathological changes.ResultsCompared to the control group, the short-term and prolonged ischemia groups showed significant increases in the plasma levels of DAO, whereas the plasma citrulline levels decreased significantly. Prolonged ischemia caused a larger increase in the plasma DAO levels and a larger decrease in the plasma citrulline levels compared to the short-term ischemia (p=0.011 and p=0.021, respectively). Intestinal damage was shown to develop more in the prolonged ischemia group (p=0.001).ConclusionIn the early period of AMI, the plasma DAO levels increase while citrulline levels decrease, and the extent of these changes depends on the duration of ischemia.

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Analysis of serum enzyme levels in a rabbit model of acute mesenteric ischemia

Cited by 10 publications

References 12 publications

Hepatic and Abdominal Carbon Dioxide Measurements Detect and Distinguish Hepatic Artery Occlusion and Portal Vein Occlusion in Pigs

Hepatic and Abdominal Carbon Dioxide Measurements Detect and Distinguish Hepatic Artery Occlusion and Portal Vein Occlusion in Pigs

Role of serum lactic dehydrogenase, glutamic oxaloacetic transaminase, creatine phosphokinase, alkaline phospatase, serum phosphorus in the cases of bowel ischemia in acute abdomen

A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia

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