A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia

Abstract

IntroductionThere is no valid and reliable diagnostic test for early diagnosis of acute mesenteric ischemia (AMI). The aim of this study was to measure the plasma levels of diamine oxidase (DAO) and citrulline in AMI to gain insight into its early diagnosis.Material and methodsA total of 21 Wistar albino rats were divided into three groups, that is, control group, short-term ischemia group, and prolonged ischemia group. The superior mesenteric artery was occluded for 15 min in the short-term ischemia group and for 12 h in the prolonged ischemia group. Twelve hours later, the experiment was terminated and plasma DAO and citrulline levels were measured. Intestinal tissue was evaluated for the histopathological changes.ResultsCompared to the control group, the short-term and prolonged ischemia groups showed significant increases in the plasma levels of DAO, whereas the plasma citrulline levels decreased significantly. Prolonged ischemia caused a larger increase in the plasma DAO levels and a larger decrease in the plasma citrulline levels compared to the short-term ischemia (p=0.011 and p=0.021, respectively). Intestinal damage was shown to develop more in the prolonged ischemia group (p=0.001).ConclusionIn the early period of AMI, the plasma DAO levels increase while citrulline levels decrease, and the extent of these changes depends on the duration of ischemia.

The effect of the antioxidant drug U-74389G on urea levels during ischemia reperfusion injury in rats

“…Xu et al 8 found urea serum concentrations significantly elevated after 45 min of kidneys IR in male Sprague-Dawley rats. Cakmaz et al 9 found the extent of increased plasma D-amino acid oxidase levels and the one of decreased citrulline levels dependent on superior mesenteric artery IR in Wistar albino rats. Koetting et al significantly enhanced 10 the renal function by 2 h hypothermic machine reperfusion with oxygenation HR-O = more than threefold increase in renal clearances of urea levels, after cold stored kidneys preservation; in female German Landrace pigs.…”

A comparative study of the antioxidant and intestinal protective effects of extracts from different parts of Java tea (Orthosiphon stamineus)

“…Plasma DAO concentrations significantly increase following intestinal mucosal damage. Thus, plasma DAO levels can serve as a marker of mucosal integrity (Çakmaz et al., ). This means that orally administered O. stamineus could protect intestinal mucosa from stress‐induced damage, with no significant difference between the effects of the root, stem, and leaf extracts.…”

Diagnosis and Treatment of Syndrome of Intestinal Insufficiency in Acute Bleeding from Upper Gastrointestinal Tract

“…Khadaroo et al [5] доказали, что повышение концентрации в плазме крови кишечных альфа-дефенсинов и кишечной жирной кислоты свя-зывающей белок (I-FABP), напрямую связано с ишемическими поражениями стенки кишки. В свою очередь R. Cakmaz et al [12] отмечают, что уже через 15 минут после пережатия верх-ней брыжеечной артерии у крыс значительно (р=0,001) увеличивается в плазме крови уровень диаминоксидазы (фермента класса оксидоре-дуктаз), а уровень цитруллина (αаминокислоты) снижается (р=0,001). Швейцарские ученые ис-пользовали в качестве биомаркеров кишечной ишемии метаболомики [13].…”