Serum cytokine/chemokine profiles in patients with dengue fever (DF) and dengue hemorrhagic fever (FHD) by using protein array

Oliveira, Renato Antônio dos Santos; Cordeiro, Marli Tenório; Moura, Patrícia; Filho, Paulo Neves Bapti Baptista; Braga-Neto, Ulisses; Marques, Ernesto T. A.; Gil, Laura Helena Vega Gonzales

doi:10.1016/j.jcv.2017.02.007

Cited by 19 publications

(22 citation statements)

References 39 publications

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“…Studies have shown high levels of IL-6 in dengue infected liver sections and elevated IL-10 levels in patients with severe dengue (Oliveira et al, 2017;Pandey et al, 2015). Consistent with other findings (Oliveira et al, 2017;Pandey et al, 2015;Flores-Mendoza et al, 2017), we observed high levels of IFN-g, IL-6 and IL-10 late in the course of DENV2 infection. Although IL-10 was generally regarded as an anti-inflammatory cytokine, several studies suggested that IL-10 contributed to antitumor immunity by assisting the expansion of tumor-resident CD8 + T cells (Emmerich et al, 2012;Mumm et al, 2011).…”

Section: Discussionsupporting

confidence: 92%

“…We know that cytokines are associated with severe dengue disease (Oliveira et al, 2017;Pandey et al, 2015;Eppy et al, 2016;Tsai et al, 2014). Studies have shown high levels of IL-6 in dengue infected liver sections and elevated IL-10 levels in patients with severe dengue (Oliveira et al, 2017;Pandey et al, 2015). Consistent with other findings (Oliveira et al, 2017;Pandey et al, 2015;Flores-Mendoza et al, 2017), we observed high levels of IFN-g, IL-6 and IL-10 late in the course of DENV2 infection.…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

“…We know that cytokines play a crucial role in the pathogenesis of dengue, which leads to severe dengue disease (Oliveira et al, 2017;Pandey et al, 2015). To further understand the kinetics of the immune response to DENV2 with respect to secretion of cytokines, we detected the serum cytokine levels in DENV2 patient at different days after diagnosis.…”

Section: Cxcr5 + Cd8 + T Cells Were Negatively Association With Denv2mentioning

confidence: 99%

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Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

Qiu¹,

Wang²,

Yu³

et al. 2019

International Journal of Infectious Diseases

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Follicular CXCR5 + CD8 + T cells have antiviral effects in chronic virus infection, but the roles of these cells during dengue virus 2 (DENV2) infection remain poorly understood. Objective: This study was conducted to analyzed in detail the dynamic changes and functional properties of circulating follicular CXCR5 + CD8 + T cells to explore their effects on DENV2 infection. Methods: Circulating follicular CXCR5 + CD8 + T cells and cytokines were analyzed by flow cytometry in DENV2 patients at difference days after DENV2 infection. CD8 + T cells were isolated and purified from DENV2 patients, then were stimulated with NS1 peptides and TCR stimulant. After cultivation, multiple parameters were tested. Results: (1) CXCR5 + CD8 + T cells emerged after DENV2 infection, with high PD-1 expression, and were correlated with the reduction in DENV2 RNA viral loads. (2) PD-1 + CXCR5 + CD8 + T cells were negatively associated with disease progression. (3) Serum IFN-g, IL-6 and IL-10 levels were increased late in the course of DENV2 infection. (4) CXCR5 + CD8 + T cells from DENV2 patients exhibited increased cytotoxicity and IFN-g and IL-10 secretion. Conclusion: CXCR5 + CD8 + T cells could play a protective role in dengue pathogenesis and may be a novel strategy for controlling DENV2 infection and vaccine development.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Cxcr5 + Cd8 + T Cells Were Negatively Association With Denv2mentioning

confidence: 99%

See 2 more Smart Citations

Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

Qiu¹,

Wang²,

Yu³

et al. 2019

International Journal of Infectious Diseases

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“…The cytokine and chemokine levels in the acute phase plasma of the DF and DHF patients, as well as healthy donors, were evaluated using Bio-Plex Human Cytokine kits (Bio-Plex Human Cytokine Assay). The cytokines and chemokines selected for analysis in this study were those investigated in other previous studies (Bozza et al, 2008;Malavige et al, 2012;Oliveira et al, 2017). A total of 12 cytokines suggested to be important biomarkers correlated with dengue disease were selected for this study.…”

Section: Cytokine/chemokine Analysismentioning

confidence: 99%

A clinical and epidemiological survey of the largest dengue outbreak in Southern Taiwan in 2015

Wang

Lin

Chang

et al. 2019

International Journal of Infectious Diseases

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This study examined the epidemiological, clinical, and immunological characteristics of the 2015 dengue outbreak in Taiwan. Methods: Clinical data were collected from dengue fever (DF) and dengue hemorrhagic fever (DHF) patients. A phylogenetic tree was used to analyze the source of the outbreak strain. Paired plasma samples from DF/DHF patients were used for antibody-dependent enhancement (ADE) assay and cytokine multiplex biometric immunoassay to validate the immunological mechanism. Results: This outbreak mainly occurred in two of the southern cities of Taiwan: Tainan (n = 22 777; 52%) and Kaohsiung (n = 19 784; 45%). A high DHF death rate was noted (34.6%). The case (DHF) and control (DF) study indicated that older age (>60 years), type II diabetes, and hypertension were risk factors correlated with the development of DHF (p < 0.0001). The phylogenetic tree results suggested that the outbreak-associated strain was dengue virus serotype 2 and cosmopolitan genotype, forming a stable cluster with the isolates from Thailand and Indonesia (bootstrap value of 99%). Cytokine analyses demonstrated that levels of interleukin (IL)-6, IL-4, IL-13, IL-1β, interferon gamma (IFN-g), and granulocyte-macrophage colony-stimulating factor (GM-CSF) were significantly higher in DHF patients compared to DF patients (p < 0.001). The ADE assay showed that diluted plasma containing preexisting dengue antibodies from DHF patients significantly enhanced dengue infection (p < 0.05). Conclusion: The results suggest that older age, type II diabetes, hypertension, immunological cytokine dysregulation, and preexisting dengue antibodies inducing ADE infection are correlated with dengue severity. This study also indicates that the largest dengue outbreak in Taiwan might have been a result of imported DF from dengue epidemic regions.

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Role of cytokines produced by T helper immune-modulators in dengue pathogenesis: A systematic review and meta-analysis

et al. 2021

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Serum cytokine/chemokine profiles in patients with dengue fever (DF) and dengue hemorrhagic fever (FHD) by using protein array

Cited by 19 publications

References 39 publications

Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

Protective role of follicular CXCR5+CD8+ T cells against dengue virus 2 infection

A clinical and epidemiological survey of the largest dengue outbreak in Southern Taiwan in 2015

Role of cytokines produced by T helper immune-modulators in dengue pathogenesis: A systematic review and meta-analysis

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