Serum creatine kinase bb linked to immunoglobulin G

Eigenschaften und Bedeutung von Makro-Kreatinkinasen

“…mit der Ouchterlony-Tedmik (Tab. 3) und in der quantitativen Immunpräzipitation Immunglobulin G als makromolekularer Bindungspartner der Kreatinkinase-BB ermitteln (10). Auch in der Präzipi-tatsichel, die sich zwischen dem Serum Rei.…”

Stability of Macro Creatine Kinases and Creatine Kinase Isoenzymes Compared: Heat Inactivation Test for Determination of Thermostable Creatine Kinases

“…Furthermore, we ascertained that macro creatine kinase is more stable when subjected to pH shifts. These observations could explain the persistence of serum creatine kinase activity in patients with macro creatine kinasaemia (4,5,6,7). Obviously macro creatine kinases are also protected from in vivo degradation.…”

“…One form of macromolecular creatine kinase demonstrated in patients' sera was identified as a complex between creatine kinase BB and immunoglobulins (4)(5)(6)(7)(8).…”

“…These sera were investigated further by exclusion chromatography on Sephadex G-200 (8) or Sephacryl S-300 sf (210 X 9 mm) to confirm the macro molecular form of creatine kinase. In every case we identified macro creatine kinase by electrophoresis in agarose gel, by ion exchange chromatography and by immunological methods with specific antisera against creatine kinase isoenzymes and immunoglobulins as described elsewhere (5,8). Furthermore we characterized two different forms of macro creatine kinases (types 1 and 2) by their binding properties towards radiolabeled creatine kinase isoenzymes (radioelectrophoresis) and protein A-Sepharose CL4B, and by their Michaelis constants and activation energies'(9,10).…”

CK-MB: A Valuable Test?

“…The peak of CK-MB activity is usually [12][13][14] h after the onset, but there can be wide variation. However, only electrophoresis is really specific for MB.…”