Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography

Abstract

Cytokines play a key role in the pathogenesis of coronary artery disease (CAD). The aim of current study was to investigate the relationship between the serum concentrations of 12 cytokines with mortality and extent of CAD in individuals undergoing angiography and healthy controls. 342 CAD patients were recruited and divided into 2 groups: those with ≥50% occlusion in at least one coronary artery [Angiography (+)] or <50% obstruction in coronary arteries [Angiography (-)]. Also 120 healthy subjects were enrolled as control group. Lipid profile, fasting blood glucose, body mass index, and blood pressure were evaluated in all the subjects. An Evidence Investigator was used for measuring 12 cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, MCP-1, IFN-γ, EGF, VEGF) using sandwich chemiluminescent assays. Univariate analysis, multivariate regression models, ROC, and Kaplan-Meier survival curves were used for exploring the candidate markers in CAD patients. Serum level of IFN-γ, IL-4, MCP-1, EGF, IL-6, and IL-8 were markedly higher in angiogram-positive patients, while VEGF concentrations were significantly (P < 0.05) lower, compared to control group. ROC analysis for MCP-1 showed that a cut-off of 61.95 pg/mL had 91% sensitivity and 91% specificity for predicting CAD patients. Moreover, >2.16 pg/mL IL-6 had a > 94% sensitivity and 70% specificity in predicting 2 years mortality in the subjects with a serum MCP-1 > 61.95 pg/ mL, and patients having IL-6/MCP-1 combination had a shorter survival.Our findings demonstrate that CAD patients with serum MCP-1 and IL-6 levels of >61.95 and >2.16 pg/mL had a higher mortality with 94.1% sensitivity and 70.5% specificity for predicting mortality in CAD patients.

Overexpression of MicroRNA-16 Alleviates Atherosclerosis by Inhibition of Inflammatory Pathways

“…This hypothesis was confirmed in our clinical and in vivo study as well as our previous in vitro research [ 12 ]. More importantly, in this in vivo study, we not only analyzed the IL-6, TNF- α , IL-10, and TGF- β in plasma and tissues of ApoE-/- mice as in vitro experiments but also included the detection of MCP-1 and IL-1 β which are implicated to be particularly important biomarkers for CAD diagnosis and mortality prediction [ 28 , 29 ].…”

“…This hypothesis was confirmed in our clinical and in vivo study as well as our previous in vitro research [12]. More importantly, in this in vivo study, we not only analyzed the IL-6, TNF-α, IL-10, and TGF-β in plasma and tissues of ApoE-/-mice as in vitro experiments but also included the detection of MCP-1 and IL-1β which are implicated to be particularly important biomarkers for CAD diagnosis and mortality prediction [28,29]. Furthermore, our present study also showed upregulated miR-16 may exert anti-inflammatory roles in AS by suppressing the transcription of PDCD4 and then activated p38 and ERK1/2, but inactivated the JNK pathway.…”

miR-126 in Peripheral Blood Mononuclear Cells Negatively Correlates with Risk and Severity and is Associated with Inflammatory Cytokines as well as Intercellular Adhesion Molecule-1 in Patients with Coronary Artery Disease

“…The results confirmed that the relative expression of miR-126 was negatively associated with the Gensini score, suggesting a reverse correlation of miR-126 with the severity of CAD. Furthermore, inflammatory cytokines are also reported to be positively correlated with the disease severity [41,42,43]. The reverse correlation of miR-126 with Gensini score might be explained as follows: (1) as an anti-inflammatory factor, miR-126 suppress inflammatory responses, possibly through downregulating inflammatory cytokines and upregulating anti-inflammatory cytokines [31,32,33]; (2) miR-126 could decrease the endothelial function-related molecules, including VEGF, ICAM-1, and VEGF [31,38,39,44].…”

Contemporary diagnostic algorithm for coronary artery disease: achievements and prospects

“…Эти исследования ведутся для выделения лиц с высо-ким риском осложненного течения сердечно-сосудистой патологии, а также для стратификации риска у условно здоровых лиц. Сегодня в литературе можно найти результаты исследований таких биомаркеров, как трансформирующий фактор роста бета (TGF-beta1) [34], молекулы клеточной адгезии (САМ) [35], моноцитарный хемоаттрактантный белок-1 (МСР-1) [36], фактор стромальных клеток 1a (SDF-1a) [37], лектиноподобный рецептор окисленных липопротеинов низкой плотности 1 (LOX-1) [38], пентраксин 3 (PTX3) [39], а также билирубин [40,41] и гликированный гемоглобин A1c (HbA 1c ) [42], чьи концентрации в сыворотке и/или плазме крови, по ряду исследовательских работ, возможно, связаны с наличием и тяжестью ИБС.…”