miR-126 in Peripheral Blood Mononuclear Cells Negatively Correlates with Risk and Severity and is Associated with Inflammatory Cytokines as well as Intercellular Adhesion Molecule-1 in Patients with Coronary Artery Disease

Wu, Hui-liang; Zhang, Jing

doi:10.1159/000484236

Cited by 15 publications

(11 citation statements)

References 44 publications

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“…In addition, we also found that miR‐218 overexpression alleviated the inflammatory injury of CMECs damaged by CAD. MiR‐218 might negatively modulate HMGB1 expression in CAD inflammatory cytokines that are produced by monocytes, endothelial cells, or adipocytes, inducing or inhibiting the inflammation in diverse inflammatory diseases (Wu & Zhang, ). Besides, inflammation is a major factor for the pathogenesis of atherosclerosis, in which the inflammatory cytokines that are produced by macrophages or monocytes are pivotal triggers (Warnatsch, Ioannou, Wang, & Papayannopoulos, ).…”

Section: Discussionmentioning

confidence: 99%

Upregulation of microRNA‐218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1

Gao¹,

Cui

et al. 2019

Journal Cellular Physiology

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This study is performed to examine the impacts of microRNA-218 (miR-218) on cardiac microvascular endothelial cells (CMECs) injury induced by coronary artery disease (CAD). Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was applied for detecting miR-218 expression in serum of patients with CAD and healthy controls, and the correlation between miR-218 expression and the clinical indexes such as creatine kinase, creatine kinase-myocardial band, cardiac troponin I, and coronary Gensini score was analyzed. CMECs were coincubated with homocysteine for 24 hr for CMECs injury, and the cells were transfected with miR-218 mimics or miR-218 inhibitors. Besides, we used oxidized low density lipoprotein as an inducer to incubate with CMECs for 24 hr, and the model of CMECs injury was established to be transfected with miR-218 mimics. RT-qPCR and western blot analysis were used to detect miR-218 and HMGB1 expression in CMECs.

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Section: Discussionmentioning

confidence: 99%

Upregulation of microRNA‐218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1

Gao¹,

Cui

et al. 2019

Journal Cellular Physiology

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“…Additionally, miR-126 has also been associated with the vascular smooth muscle cell (VSMC) turnover related to plaque thinning, and response to shear stress (Zhou et al, 2013). Recent investigations have demonstrated an association between CVD events and a decrement of miR-126 expression levels in different populations (Zampetaki et al, 2010;Fukushima et al, 2011;Kontaraki et al, 2014;Wu and Zhang, 2018). Zampetaki et al (2012), for example, found a decrement in the circulating miR-126 levels in individuals with type 2 diabetes compared to the control group Zampetaki et al (2012a).…”

Section: Plasma Mirnas Expression Levelsmentioning

confidence: 99%

Expression levels of circulating microRNAs‐126, ‐155, and ‐145 in Mexican women exposed to polycyclic aromatic hydrocarbons through biomass fuel use

Ruíz-Vera

Ochoa-Martínez

Pruneda-Álvarez

et al. 2019

Environ and Mol Mutagen

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Human exposure to polycyclic aromatic hydrocarbons (PAHs) has been considered a risk determinant for the development of cardiovascular diseases (CVD). Therefore, the aim of this study was to assess expression levels of vascular‐related miRNAs, miR‐126, miR‐155, and miR‐145, in plasma from women (aged 19–81 years) exposed (n = 100) and non‐exposed (n = 20) to PAHs via biomass combustion smoke.1‐hydroxypyrene (1‐OHP) was determined in urine as a biomarker of exposure to PAHs using high‐resolution liquid chromatography. Plasma expression levels of proposed miRNAs were determined by quantitative real‐time PCR. Additionally, traditional risk factors (age, blood pressure, serum lipid profile, blood glucose, and among others) associated with CVD were evaluated. Urinary 1‐OHP concentrations and plasma expression levels of miR‐126 and miR‐155 were significantly higher (P < 0.05) in women using wood as a fuel source in their homes (indoor) compared to women from the reference group (non‐exposed to biomass smoke). Besides, multivariate linear regression analyses revealed that miR‐126[β = 0.61; 95% confidence interval (0.32–0.90)] and miR‐155 [β = 0.45; 95% confidence interval (0.13–0.84)] expression levels were significantly associated with urinary 1‐OHP concentrations after being adjusted by traditional risk factors (P < 0.05). In contrast, no significant relationship was found between miR‐145 and urinary 1‐OHP levels. Furthermore, miRNAs assessed in this investigation are associated with CVD events. Consequently, actions to reduce exposure to PAHs in the evaluated population are warranted. Environ. Mol. Mutagen. 60:546–558, 2019. © 2019 Wiley Periodicals, Inc.

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“…One study showed that the AUC of miRNA-126 in diagnosing CAD was 0.98 ( 24 ). The predictive value of miR-126 for CAD may be explained by its essential roles in the mediation of endothelial cell activities and inflammatory responses ( 32 ). Moreover, miRNA-223 and miRNA-149 have relatively good potential as biomarkers for the diagnosis and prognosis of CAD patients, with diagnostic AUCs of 0.933 and 0.938, respectively ( 28 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

The diagnostic value of circulating microRNAs as biomarkers for coronary artery disease: A meta‑analysis

Wang¹

2020

Anatol J Cardiol

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Objective: In recent years, research on microRNAs (miRNAs) associated with coronary artery disease (CAD) has attracted considerable attention. However, findings of these studies on the validity of circulating miRNAs in CAD diagnosis are controversial. A meta-analysis was therefore conducted to determine the potential value of miRNAs as biomarkers in CAD diagnosis. Methods: Relevant documents on miRNAs expression levels in the diagnosis of CAD were searched and collected from Pubmed, Embase, and Web of Science. They were collected from the time of inception of the database till January 31, 2020. A meta-analysis was conducted using Stata14.0 software. Forest maps were studied and a comprehensive evaluation of the diagnostic value of the expression levels of mRNAs in CAD was conducted using statistical indicators such as the summary receiver operating characteristic curve. Results: Overall, 14 studies were included, with 38 data sets, involving 29 miRNAs with 846 cases and 898 controls. The meta-analysis revealed that the average sensitivity and specificity of miRNAs for CAD diagnosis were 0.80 (0.75–0.84) and 0.78 (0.75–0.81), respectively. The positive likelihood, negative likelihood, and diagnostic odds ratios were 3.7 (3.1–4.4), 0.26 (0.21–0.33), and 14 (10–21), respectively, and the area under the curve was 0.85 (0.82–0.88). Subgroup analysis revealed that the accuracy in the Asian population was higher than that in the non-Asian population. Multiple miRNAs may be more diagnostically accurate than single miRNAs. MiRNAs in whole blood were more accurate than those in plasma, serum, and peripheral blood mononuclear cells. The diagnostic performance of the quantitative real-time polymerase chain reaction group was better than that of the qPCR group. Conclusion: According to our study, miRNAs may be a new, non-invasive diagnostic tool for the diagnosis of CAD. As a screening tool in clinical practice, it has potential diagnostic value and is worthy of clinical promotion. Considering the number and quality of the studies included in this meta-analysis, the above conclusion requires more quality research to verify it.

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miR-126 in Peripheral Blood Mononuclear Cells Negatively Correlates with Risk and Severity and is Associated with Inflammatory Cytokines as well as Intercellular Adhesion Molecule-1 in Patients with Coronary Artery Disease

Cited by 15 publications

References 44 publications

Upregulation of microRNA‐218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1

Upregulation of microRNA‐218 reduces cardiac microvascular endothelial cells injury induced by coronary artery disease through the inhibition of HMGB1

Expression levels of circulating microRNAs‐126, ‐155, and ‐145 in Mexican women exposed to polycyclic aromatic hydrocarbons through biomass fuel use

The diagnostic value of circulating microRNAs as biomarkers for coronary artery disease: A meta‑analysis

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