Serum Concentrations of Acute Phase Proteins and Bone Biomarkers in Female Dromedary Camels During The Periparturient Period

Tadjine, Mohamed; Al-Sobayil, Fahd

doi:10.5958/2277-8934.2015.00045.4

Cited by 12 publications

(7 citation statements)

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“…The APPs are a group of blood proteins that change in concentration in animals subjected to external or internal challenges such as infection, inflammation, surgical trauma, or stress [7]. The changes in APPs due to various inflammatory and non-inflammatory conditions have been studied in many animal species [7,[23][24][25][26][27]. In this study, the serum concentrations of the APPs SAA and Hp were significantly higher in both the jugular and mammary veins compared to values of same veins in healthy control goats.…”

Section: Mammary Veinmentioning

confidence: 66%

Jugular versus Mammary Vein Acid-Base Balance, Blood Gases, Hematobiochemical Profiles and Inflammation Biomarkers in Goats with Acute Mastitis

Tharwat¹,

Al-Sobayil²,

Almundarij³

2017

Journal of Veterinary Science and Animal Husbandry

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Section: Mammary Veinmentioning

confidence: 66%

Jugular versus Mammary Vein Acid-Base Balance, Blood Gases, Hematobiochemical Profiles and Inflammation Biomarkers in Goats with Acute Mastitis

Tharwat¹,

Al-Sobayil²,

Almundarij³

2017

Journal of Veterinary Science and Animal Husbandry

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“…Quantification limits of the assay ranged from 2 to 32 ng/mL, 2-140 U/L and 15-750 nmol/L for OC, β-ALP and PYD, respectively. Precision CVs within and between runs were 5-10% for OC, 4-6% and 5-8% for β-ALP, 6-10% and 3-11% for PYD Tharwat and Al-Sobayil 2015b;Tharwat and Al-Sobayil 2018a,b;Tharwat 2020b;Al-Sobayil and Tharwat 2021).…”

Section: Bone Biomarkers Assaymentioning

confidence: 93%

The Effects of Acute Blood Loss on Inflammatory and Bone Biomarkers, Acid-base Balance, Blood Gases and Hemato-biochemical Profiles in Sedated Donkeys (Equus asinus)

2022

Int J Vet Sci

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The current experiment was designed to investigate the possible effects of acute blood loss on the inflammatory and bone biomarkers status, acid-base balance, blood gases, and hemato-biochemical profiles in sedated donkeys (Equus asinus). For the induction of acute blood loss, collected 900mL of blood from the carotid artery of 10 male donkeys sedated with xylazine HCl (1mg/kg). For analysis, five blood samples were collected; collected the 1st (T0) before induction of blood loss and the other 4 (T1, T2, T3 and T4) collected at 30, 60, 120 and 240min post-blood loss. The serum concentrations of amyloid A (SAA) and haptoglobin (Hp) decreased at all-time points after blood loss compared to T0, but the differences were non-significant. The differences in levels of serum biomarkers osteocalcin (OC), β-alkaline phosphatase (β-ALP) and pyridinoline cross-links (PYD) at all-time points post blood loss compared to T0 values were also non-significant. Except for PCO2, the acid-base status and blood gases, including pH, PO2, HCO3, TCO2, sO2, base excess, anion gap and lactate, increased significantly after blood withdrawal. On the contrary, most of the measured hemato-biochemical parameters decreased significantly after blood loss, except for glucose. In conclusion, acute blood loss in sedated donkeys did not influence serum concentrations of the inflammatory biomarkers SAA and Hp and the markers of bone metabolism OC, β-ALP, and PYD. On the contrary, most acid-base, blood gas parameters, and hemato-biochemical parameters differed significantly after blood withdrawal compared to their values before blood loss.

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“…The inflammation biomarkers serum amyloid A (SAA) and haptoglobin (Hp) were tested in serum samples by commercial kits (Multispecies SAA ELISA kit and Hp colorimetric kit, Tridelta Ltd., Ireland) as reported ( Tharwat et al ., 2014a ; Tharwat and Al-Sobayil, 2015a , b , 2018a ; Tharwat, 2020a , 2022a , 2023 ; Al-Sobayil and Tharwat, 2021 ). In addition, the serum concentrations of the bone metabolism biomarkers osteocalcin (OC), bone alkaline phosphatase (b-ALP), and pyridinoline cross-links (PYD) were evaluated in serum samples by an immunoassay kits (Metra Biosystems Inc., a division of Quidel Corp.) as published ( Tharwat et al ., 2014a ; Tharwat and Al-Sobayil, 2015b , 2018a , b , 2022b ; Tharwat 2020b , 2023 ; Al-Sobayil and Tharwat 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Acute-phase proteins or biomarkers of infection and inflammation are proteins increased or decreased in the blood owing to infection or inflammation or trauma ( Murata et al ., 2004 ; Tharwat et al ., 2014a ; Tharwat and Al-Sobayil, 2015a , b , 2018a , 2022a ; Tharwat, 2020a , 2023 ). Biomarkers of bone metabolism are also used for assessing the bone reaction to either invasive or non-invasive interventions and for the evaluation of musculoskeletal diseases ( Frisbie et al, 2008 ; Tharwat and Al-Sobayil, 2018b , 2022a ; Tharwat, 2020b , 2023 ; Al-Sobayil and Tharwat, 2021 ).…”

Section: Introductionmentioning

confidence: 98%

Changes in the hematobiochemical, acid–base and blood gas elements as well as biomarkers of inflammation and bone metabolism in donkeys (Equus asinus) with acute bleeding

Tharwat,

Sobayil,

Ali

2024

Open Vet J

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Background: Acute hemorrhage is fatal in equines with a complication of severe hypovolemic shock that causes a sudden death in such cases. Aim: This study was designed to report the influences of acute bleeding in conscious non-sedated donkeys (Equus asinus) on the hematobiochemical variables, acid-base, blood gas elements and markers of inflammation and bone metabolism. Methods: Eight healthy donkeys were used where a total of 900mL of whole blood was collected. Five blood samples were collected from each animal: just before collection of blood (T0); (2) 30min (T1), 60min (T2), 120min (T3) and 240min (T4) later. The blood panels including total white blood cells, lymphocytes, neutrophils, red blood cell counts (RBCs), HCT, hemoglobin (Hg) and RBCs indices were measured. Biochemical parameters and electrolytes were evaluated. The activity of aspartate aminotransferase (AST), creatine kinase (CK) and γ-glutamyl transferase (GGT) were also determined. Complete acid-base and blood gas panel were assessed. Serum amyloid A (SAA), haptoglobin (Hp), osteocalcin (OC), bone alkaline phosphatase (b-ALP) and pyridinoline cross-links (PYD) were measured. Results: The RBCs, Hg and HCT increased significantly at points T1, T2 and T3 compared to T0. The concentrations of total proteins and albumin decreased significantly at points T3 and T4. The blood urea nitrogen concentrations increased significantly at T4. Creatinine concentrations increased significantly at T2 and T3. The AST, GGT and CK decreased significantly. On the other hand, glucose increased significantly at T3 and T4. The pH decreased significantly at points T1, T2, T3 and T4. The PCO2 increased significantly at T 3 and T4. The BE, HCO3 and TCO2 values decreased significantly at T2, T3 and T4. Contrary, the AG increased significantly at points T3 and T4. The potassium increased significantly at T1-T4 and chloride decreased significantly at T3 and T4. Lactate showed significant increases at T1-T4. The SAA, Hp, OC, b-ALP and PYD did not differ significantly at T1-T4. Conclusion: In conscious non-sedated donkeys, induced bleeding resulted in significant changes in the hematobiochemical elements, the acid-base status and blood gas and electrolyte parameters. However, it did not change the markers of inflammation and bone metabolism.

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Serum Concentrations of Acute Phase Proteins and Bone Biomarkers in Female Dromedary Camels During The Periparturient Period

Cited by 12 publications

References 0 publications

Jugular versus Mammary Vein Acid-Base Balance, Blood Gases, Hematobiochemical Profiles and Inflammation Biomarkers in Goats with Acute Mastitis

Jugular versus Mammary Vein Acid-Base Balance, Blood Gases, Hematobiochemical Profiles and Inflammation Biomarkers in Goats with Acute Mastitis

The Effects of Acute Blood Loss on Inflammatory and Bone Biomarkers, Acid-base Balance, Blood Gases and Hemato-biochemical Profiles in Sedated Donkeys (Equus asinus)

Changes in the hematobiochemical, acid–base and blood gas elements as well as biomarkers of inflammation and bone metabolism in donkeys (Equus asinus) with acute bleeding

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