Serum Biomarkers of Renal Cell Carcinoma Assessed Using a Protein Profiling Approach Based on ClinProt Technique

Alterations of the serum peptidome in renal cell carcinoma discriminating benign and malignant kidney tumors

“…small pilot study a cluster of peptides able to discriminate RCC patients from healthy subjects thus encouraging the prosecution of this investigation with a wider number of cases based on magnetic bead pre-fractionation before MALDI-TOF analysis both on serum and urine [22,23]. Here we present a proteomic study based on serum peptidome profiling with ClinProt-MALDI-TOF in Renal Cell Carcinoma.…”

“…only for RCC [22,23], but also for different type of tumors [24]. A technique based on activated target surface, well known as SELDI-MALDI-TOF, has also been successfully used to provide preliminary evidence that a cluster of peptides can be used to build a decision tree flow able to differentiate not only ccRCC from normal subjects [25], but also from benign renal tumors in combination with CT [26].…”

“…The receiver operating characteristic curve analysis and area under the curve (AUC) in order to determine the diagnostic efficacy of each single marker were evaluated as previously described [23]. Two pools of sera from 80 patients and 80 healthy subjects were used to evaluate patients/controls peptide ratios by LC-ESI.…”

Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis

“…The successful discovery of a proteomic profi le correlated to an altered state by the ClinProt method has been reported in various human diseases, such as oral, bladder, nasopharyngeal and neck cancer (13)(14)(15). Recently, we have used this approach to fi nd possible ccRCC biomarkers in urine and serum (12,16). In view of these results, we investigated the possibility of using the ClinProt technique to fi nd possible plasma diagnostic markers that can better distinguish healthy subjects from patients affected by SCCP.…”

Comparative Serum Proteomic Analysis of Serum Diagnosis Proteins of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry

“…It is being interestingly recognized that low mass weight peptides, such as S100A8 and fibrinogen, play an important role in physiological and pathological process and could be used as relevant biomarker candidates (Cheng et al, 2005;Tolson et al, 2006). Recently mass spectrum that directly detects and differentiates short peptides has offered a promising approach for peptidomic biomarker discovery (Alagaratnam et al, 2008;Chinello et al, 2010;Dai et al, 2010;Du et al, 2010;Liu et al, 2010).…”