Serum Biochemical Parameters, Rumen Fermentation, and Rumen Bacterial Communities Are Partly Driven by the Breed and Sex of Cattle When Fed High-Grain Diet

Qiu, Xiaobing; Qin, Xiaoli; Chen, Liming; Chen, Zhiming; Hao, Rikang; Zhang, Siyu; Yang, Shunran; Wang, Lina; Cui, Yafang; Li, Yingqi; Ma, Yiheng; Cao, Binghai; Su, Huawei

doi:10.3390/microorganisms10020323

Cited by 28 publications

(28 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, Prevotellaceae and Rikenellaceae (Bacteroidetes), Fibrobacteraceae (Fibrobacteres), Lactobacillaceae (Firmicutes), WCHB1_41 (Verrucomicrobia), and Kiritimatiellaeota were significantly enriched in the feces of Thoroughbred horses. Prevotellaceae has enzymes and gene clusters capable of fermenting and utilizing complex polysaccharides, and the abundance of the bacterial family in the gut is influenced by breed and gender ( 31 ). The RC9 gut group of Rikenellaceae increased the apparent digestibility of acid detergent fiber and neutral detergent fiber, thus improving the dry matter intake of animals ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Luo

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

The horse gut is colonized by a rich and complex microbial community that has important roles in horse physiology, metabolism, nutrition, and immune functions. Fewer across-breed variations in horse gut microbial diversity have been illustrated. In this article, the gut microbiota of Thoroughbred, Mongolian, and Hybrid horses [first filial generation (F1) of Mongolian (maternal) and Thoroughbred (paternal)] were studied by second-generation high-throughput sequencing technology. Differences in gut microbiota composition and function between breeds were determined using diversity and functional prediction analysis. The alpha diversity analysis showed that Thoroughbred horses had a more abundant and diverse gut microbiota, while the diversity of gut microbiota in Hybrid horses was intermediate between Thoroughbred and Mongolian horses. Subsequent cluster analysis showed that Hybrid horses have a microbiota composition more similar to Mongolian horses. LEfSe analysis revealed that the bacterial biomarkers for Thoroughbred horses at the family level were Prevotellaceae, Rikenellaceae, Fibrobacteraceae, p_251_o5, Lactobacillaceae, and uncultured_bacterium_o_WCHB1_41; the bacterial biomarker for Mongolian horses was Planococcaceae; and the bacterial biomarkers for Hybrid horses were Moraxellaceae, Enterobacteriaceae, and Ruminococcaceae. The functional prediction results indicated that the metabolic pathways differ significantly between the breeds. Regarding metabolism, the Hybrid horses had the lowest proportion of the carbohydrate metabolic pathways, while the energy metabolic pathway had the highest proportion. The abundance ratios of the remaining eight metabolic pathways in Hybrid horses were between Thoroughbred and Mongolian horses. In conclusion, the results of this study showed an association between horse breeds and gut microbiota.

show abstract

Section: Discussionmentioning

confidence: 99%

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Luo

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…The genus Rikenellaceae_RC9_gut_group belongs to the family Rikenellaceae , which plays a role in the fermentation of carbohydrates or proteins (Su et al, 2014 ; Yan et al, 2018 ). Qiu et al ( 2022 ) found that its relative abundance was positively correlated with rumen pH, dietary fiber, and acetate to propionate ratio. However, the greater relative abundance of Rikenellaceae_RC9_gut_group was observed at high media pH in the present study and the inconsistent results may be attributed to the different animal species (dairy vs. beef cattle).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the greater relative abundance of CAG-352 at high media pH was noticed, but the role of CAG-352 during rumen fermentation is still limited. A recent study demonstrated a negative correlation between the relative abundance of CAG-352 with serum concentration of glucose and a positive correlation with serum concentrations of creatinine, total protein, albumin, alkaline phosphatase, and non-esterified fatty acid in cattle (Qiu et al, 2022 ). In addition, the changes in relative abundances of several species at phylum and genus levels with supplementation of C. butyricum at both media pH levels in the current study may be contributed by the nutritional factors (enzymes and vitamins) produced by C. butyricum .…”

Section: Discussionmentioning

confidence: 99%

Effect of Clostridium butyricum Supplementation on in vitro Rumen Fermentation and Microbiota With High Grain Substrate Varying With Media pH Levels

Jiao

Wang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Clostridium butyricum (C. butyricum) can survive at low pH, and it has been widely used as an alternative to antibiotics for the improvement of feed efficiency and animal health in monogastrics. A recent study suggested that the improved ruminal fermentation with supplementing C. butyricum is may be associated with increasing the abundance of rumen microbiota in Holstein heifers, as ruminal pH plays a key role in rumen microbiota and the probiotics are often active in a dose-dependent manner. The objective of this study was to determine the effects of increasing the doses of C. butyricum on gas production (GP) kinetics, dry matter disappearance (DMD), fermentation characteristics, and rumen microbiota using a high grain substrate in batch culture varying with media pH levels. The doses of C. butyricum were supplemented at 0 (control), 0.5 × 106, 1 × 106, and 2 × 106 CFU/bottle, respectively, at either media pH 6.0 or pH 6.6. The fermentation microbiota at 0 and 1 × 106 CFU/bottle were determined using the 16S rRNA high throughput sequencing technology. Overall, the GP, DMD, total volatile fatty acid (VFA) concentration, and the ratio of acetate:propionate were higher (P <0.01) at media pH 6.6 than at pH 6.0. However, there was interaction between pH × dose of C. butyricum for rate constant of GP (P = 0.01), average GP rate (P = 0.07), and volume of GP (P = 0.06); with the increase in C. butyricum supplementation, the GP kinetics were not changed at media pH 6.0, but the volume (P = 0.02), rate of GP (P = 0.01), and average GP rate (P = 0.01) were quadratically changed at media pH 6.6. The DMD was not affected by increasing the supplementation of C. butyricum. The molar proportions of propionate (P <0.09), butyrate (P <0.06), and NH3-N concentration (P = 0.02) were quadratically changed with increasing supplementation of C. butyricum regardless of media pH levels. The interactions between media pH level and dose of C. butyricum supplementation were noticed for alpha diversity indexes of Shannon (P = 0.02) and Evenness (P = 0.04). The alpha diversity indexes increased (P <0.05) except for Chao1 with supplementation of C. butyricum. The unweighted uniFrac analysis showed that the group of control at media pH 6.0 and control at media pH 6.6, and supplementation of C. butyricum and control at media pH 6.0 clustered separately from each other. At the phylum level, relative abundance (RA) of Bacteroidota was lower (P <0.01) and Firmicutes was higher (P <0.01) at media pH 6.6 than pH 6.0. Moreover, RA of Proteobacteria decreased (P <0.05) with supplemented C. butyricum at either media pH 6.6 or pH 6.0. At media pH 6.6, RA of Rikenellaceae_RC9_gut_group and Prevotella were decreased, and CAG-352 was increased (at genus level) compared to pH 6.0. Supplementation of C. butyricum decreased RA of Rikenellaceae_RC9_gut_group and increased CAG-352 at media pH 6.0. It could hence be concluded that manipulating media pH level and supplementation of C. butyricum effectively modulated in vitro rumen fermentation characteristics and microbiota but in a dose depending manner of C. butyricum addition.

show abstract

“…The experimental results showed that supplementation with methionine does not affect the production of volatile fatty acids by microorganisms ( Table 6 ). This suggested that methionine supplementation under the present experimental conditions had little effect on the fermentative capacity of the rumen microorganisms, but the ratio of acetic acid to propionic acid changed, and we speculated that the fermentation pattern in the rumen may have changed [ 66 ]. PCoA analysis and adonis analysis based on unweighted UniFrac distance, Bray–Curtis distance, and weighted UniFrac distance matrices showed significant differences in the Bacteroidota structure among the three groups of sika deer ( Figure 3 , Table 7 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Methionine Supplementation on Serum Metabolism and the Rumen Bacterial Community of Sika Deer (Cervus nippon)

Guo

Zhao

et al. 2022

Animals

View full text Add to dashboard Cite

Methionine is the first or second limiting amino acid for ruminants, such as sika deer, and has a variety of biological functions such as antioxidant activity, immune response, and protein synthesis. This study aimed to investigate the effects of methionine supplementation on antler growth, serum biochemistry, rumen fermentation, and the bacterial community of sika deer during the antler-growing period. Twelve 4-year-old male sika deer were randomly assigned to three dietary groups supplemented with 0 g/day (n = 4, CON), 4.0 g/day (n = 4, LMet), and 6.0 g/day (n = 4, HMet) methionine. No significant difference (p > 0.05) was found in the production performance between the three groups, but antler weight was higher in both the LMet and HMet groups than in the CON group. Methionine supplementation significantly increased the serum glutathione peroxidase activity (p < 0.05). The serum immunoglobulin G level was significantly higher in the HMet group than in the other two groups (p < 0.05). No significant effect was found on the apparent amino acid digestibility of the three groups, but cysteine and methionine digestibility were higher in the LMet group. The serum hydroxylysine level was significantly lower in the LMet and HMet groups, whereas the serum lysine level was significantly lower in the HMet group compared with the CON group (p < 0.05). The LMet group had the highest but a nonsignificant total volatile fatty acid content and significantly higher microbial protein content in the rumen than the CON group (p < 0.05). The phyla Bacteroidetes, Firmicutes, and Proteobacteria were dominant in the rumen of the sika deer. The principal coordinate analysis (PCoA) and analysis of similarities (ANOSIM) results showed a significant change in the bacterial composition of the three groups (p < 0.05). The relative abundance of Prevotella and Rikenellaceae-RC9 was significantly higher in the LMet group compared with the CON group and CON and HMet groups, respectively. These results revealed that methionine supplementation improved the antioxidant activity and immune status, affecting amino acid metabolism and rumen microbial composition of the sika deer.

show abstract

Serum Biochemical Parameters, Rumen Fermentation, and Rumen Bacterial Communities Are Partly Driven by the Breed and Sex of Cattle When Fed High-Grain Diet

Cited by 28 publications

References 48 publications

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses

Effect of Clostridium butyricum Supplementation on in vitro Rumen Fermentation and Microbiota With High Grain Substrate Varying With Media pH Levels

Effect of Methionine Supplementation on Serum Metabolism and the Rumen Bacterial Community of Sika Deer (Cervus nippon)

Contact Info

Product

Resources

About