Serum bile acids after a test meal in Crohn's disease

Abstract

The serum levels of conjugated cholic and chenodeoxycholic acid have been studied before and during a 4 h period after the intake of a liquid test meal in seven control subjects and in fourteen patients with Crohn's disease. The concentrations of serum bile acids were determined by radioimmunoassay. The control group showed a postprandial increase of both conjugates with a return to the fasting level for cholic acid within 4 h. The chenodeoxycholic acid conjugate did not return to the fasting level within the test period. The serum bile acid concentration in Crohn's disease divided the patients in two groups; one group with decreased or normal fasting levels and low postprandial increase and another group with elevated fasting levels and a postprandial increase without return to the fasting levels within the test period.

Serum Bile Acids in Relation to Disease Activity and Intake of Dietary Fibers in Juvenile Ulcerative Colitis

“…This difference might be due to the methodology since our radioimmunoassay showed full cross-reac tivity for glycine and taurine conjugates and the unconjugated BA, thus revealing the to tal BA levels of S-C and S-CDC, respective ly. There was no cross-reactivity with dehydroxylated metabolites of these primary BA [19,25],…”

“…In healthy adults, children and infants, the fasting and postprandial S-CDC have been found to be higher than those of S-C and did not return to fasting levels within 150-240 min [ 19,26,27], Similar to earlier reports, serum BA values in our series of controls did not return to fasting levels within 180 min [13,28]. Serum levels were generally higher in our study compared to those reported by Balistreri et al [28] and Heubi and Balistreri [29].…”

“…180 and 240 min after intake of the test meal. S-C and S-CDC were mea sured by radioimmunoassays [19]. The method was very specific [24,25] and had a coefficient of varia tion within I day and from day to day within 7-10%.…”

“…Patients in remission were also studied after long term intake of different fibers. We used sen sitive and specific radioimmunoassays mea suring the sum of conjugated and unconju gated serum C (S-C) and serum CDC (S-CDC), respectively [19],…”

Postprandial Cholylglycine Serum Concentration and Extent of Heal Inflammation or Resection in Crohn’s Disease

“…Ewerth [4] found a normal postprandial pattern of serum cholic acid only in patients with the shortest ileal resection of 20 cm. Samuelson et al [14] have shown that in patients with re sected distal ileum < 16 cm, postprandial se rum peaks of cholic acid conjugates are simi lar to those of healthy subjects. Hofmann and Poley [7] found normal bile acid pool size in patients with resection of < 30 cm of the distal ileum.…”