Serum arylesterase and paraoxonase activity in patients with chronic hepatitis

Kılıç, Süleyman; Aydın, Süleyman; Kılıç, Nermin; Erman, Fazilet; Aydın, Suna; Çelik, İ̇lhami

doi:10.3748/wjg.v11.i46.7351

Cited by 77 publications

(53 citation statements)

References 30 publications

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“…The rate of increase in absorbance (A) was monitored for 2.5 min. The same protocol was followed in order to determine the rate of non-enzymatic hydrolysis [30].…”

Section: Serum Basal Paroxonase (Bpon) Activitymentioning

confidence: 99%

Decreased basal activity of HDL associated enzyme: Paraoxonase (PON) during uncompensated oxidative stress among type 2 diabetes mellitus patients

Dhanunjaya

Dolia

2014

Int J Diabetes Dev Ctries

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The inverse relationship between serum levels of High Density Lipoprotein (HDL) and the development of Cardio Vascular Disease (CVD) risk among diabetic patients was known for several decades. Besides the decreasing quantity of HDL, the qualitative functions of HDL are adversely affected during uncompensated oxidative stress among diabetics and leads to implication of several complications such as dyslipidemia, lipid peroxidation, endothelial dysfunction and atherosclerosis. Therefore we have undertaken this study to determine anti-atherogenic property of HDL by measuring it's one of the associated enzymes; paraoxonase (PON) among type 2 diabetes patients, along with the serum activity of superoxide dismutase (SOD) as an index of antioxidant status and lipid peroxidation end product, i.e malondialdehyde (MDA) as a marker for oxidative stress. This study included a total of 56 untreated type 2 diabetic patients and 29 healthy volunteers as controls. FBS, PPBS, HbA 1C and fasting lipid profile were measured in both the study groups. Activity of basal PON, SOD and plasma MDA levels was determined in both the study groups according to standard clinical laboratory procedures. All the diabetic patients were under poor glycemic control. Serum levels of HDL between the two study groups are not significantly differed. But, serum basal PON and SOD activity were significantly decreased, whereas MDA levels were highly elevated (284±59 nM/mL/min, 111± 35 μmol/L, 10.38±4.17 IU/mL respectively) when compared with healthy controls (371±46 nM/mL/min, 63±12 μmol/L, 16.91±2.89 IU/mL respectively). Although there is no significant reduction in concentrations of HDL in diabetics when compared with controls, but there was a significant decrease in anti-atherogenic property i.e. activity of paraoxonase enzyme. Moreover the serum activity of paraoxonase was significant and negatively correlated with MDA levels (r =-0.53, P<0.001) as well as with FBS (r=-0.30, P<0.05). Therefore the qualitative functions of HDL are significantly affected by hyperglycemia induced oxidative stress. Hence, we concluded that the quality of HDL is most important in order to determine oxidative stress related complications in diabetes mellitus than its concentration.

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“…The rate of increase in absorbance (A) was monitored for 2.5 min. The same protocol was followed in order to determine the rate of non-enzymatic hydrolysis [30].…”

Section: Serum Basal Paroxonase (Bpon) Activitymentioning

confidence: 99%

Decreased basal activity of HDL associated enzyme: Paraoxonase (PON) during uncompensated oxidative stress among type 2 diabetes mellitus patients

Dhanunjaya

Dolia

2014

Int J Diabetes Dev Ctries

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“…The basal arylesterase activity of paraoxonase (E.C.3.1.1.2; basal PON) was measured spectrophotometrically as described previously [15]. In brief, to the 50 lL of serum 3 ml of tris buffer (20 mM) was added and initial absorbance was adjusted to 0.5 in spectrometer at a wavelength of 412 nm.…”

Section: Basal Paraoxonase (Bpon) Activitymentioning

confidence: 99%

Association of Type II 5′ Monodeiodinase Thr92Ala Single Nucleotide Gene Polymorphism and Circulating Thyroid Hormones Among Type 2 Diabetes Mellitus Patients

Yalakanti

Dolia

2015

Ind J Clin Biochem

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Diabetes mellitus and thyroid disorders are common endocrinopathies, which often occur parallel. Dyslipidemia is very common in both of these conditions. The development of hypothyroidism is well-known in type 1 diabetics, but it was not distinctly understood in type 2 diabetics. Thus we tried to examine the association between type II deiodinase (D2 or DIO2) Thr92Ala single nucleotide gene polymorphism and thyroid function among type 2 diabetes mellitus patients. A total of 130 type 2 diabetics were screened and genotyped for DIO2 Thr92Ala polymorphism. Fasting plasma glucose, Glycosylated haemoglobin, lipid and thyroid profiles, malondialdehyde (MDA) and paraoxonase were estimated according to standard procedures. A significant altered level of thyroid hormones (TH's) was found in Ala/Ala genotype when compared with Thr/Thr or Thr/Ala genotype. DIO2 and T 3 :T 4 ratio significantly decreased, whereas total T 4 and thyroid stimulating hormone levels significantly elevated among Ala/Ala genotype (131 ± 30 ng/ml; 0.12 ± 0.05; 7.17 ± 2.05 lg/dl; 4.77 ± 3.1 lIU/ml, respectively) when compared with Thr/Thr ? Thr/Ala genotypes (176 ± 33 ng/ml; 0.21 ± 0.05; 5.21 ± 1.1 lg/dl; 2.59 ± 1.61 lIU/ml respectively). Moreover, D2 levels were significantly negatively correlated with TH's levels except total T 4 among Ala/Ala genotypes. All the patients were having a poor glycemic control, and their glycemic status was positively correlating with MDA levels. On the other hand, serum paraoxonase activity decreased among Ala/Ala genotype (104 ± 21 vs. 118 ± 18 nmol/min/ml). In conclusion, DIO2 Ala92 homozygous variant found to be associated with altered levels of DIO2, Thyroid profile and paraoxonase. Hence, we recommend to do detail study of genetic factors related to thyroid function and prevent additional diabetic complications.

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“…31 Yapılan çalışmalarda kronik karaciğer hastalıklarında PON1 ve nitrik oksit (NO) seviyeleri anlamlı derecede düşük, MDA seviyelerin ise yüksek bulunmuş ve hepatosteatozlu hastalarda oksidatif stresin PON1'i anlamlı derecede baskıladığı sonucuna varılmıştır. 32,33 Ferre ve ark. çalışmalarında, kronik karaciğer hastalığında PON1 aktivitesini düşük ve hasar düzeyi ile ilişkili bularak, serum PON1 aktivitesinin karaciğer hasarının belirlenmesinde önemli bir gösterge olduğu sonucuna varmışlar-dır.…”

Section: Discussionunclassified

The Relation of Paraoxonase Levels with the Histopathology of the Old Rat Liver Tissue After Repeated Desflurane Anesthesia

Cüce¹,

Yosunkaya²,

Borazan³

et al. 2013

Turkiye Klinikleri J Med Sci

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Serum arylesterase and paraoxonase activity in patients with chronic hepatitis

Abstract: Low PON1 and AE activity may contribute to the increased liver dysfunction in chronic hepatitis patients by reducing the ability of HDL to retard LDL oxidation and might be clinically useful for monitoring the disease of chronic hepatitis.

Cited by 77 publications

References 30 publications

Decreased basal activity of HDL associated enzyme: Paraoxonase (PON) during uncompensated oxidative stress among type 2 diabetes mellitus patients

Decreased basal activity of HDL associated enzyme: Paraoxonase (PON) during uncompensated oxidative stress among type 2 diabetes mellitus patients

Association of Type II 5′ Monodeiodinase Thr92Ala Single Nucleotide Gene Polymorphism and Circulating Thyroid Hormones Among Type 2 Diabetes Mellitus Patients

The Relation of Paraoxonase Levels with the Histopathology of the Old Rat Liver Tissue After Repeated Desflurane Anesthesia

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