Serum antibodies to herpes simplex virus type 1 during active oral herpes infection

Ratner, Joan J.; Smith, Kendall O.

doi:10.1128/iai.27.1.113-117.1980

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…have been noted in a recent study of human sera associated with recurrent herpes labialis (27). Antibody taken between recurrent episodes had a lower proportion of NT to RIA antibody, whereas antibody assayed during recurrent oral lesions had a higher proportion of NT to RIA antibody (higher efficiency NT antibody).…”

Section: Differences Between Nt and Ria Antibodiesmentioning

confidence: 85%

Immune responses to labial infection of BALB/c mice with herpes simplex virus type 1

Morahan¹,

Thomson²,

Kohl³

et al. 1981

Infect Immun

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The kinetics of appearance of five humoral antibody responses (micro-neutralization assay [NT], complement fixation [CF], enzyme-linked immunosorbent assay [ELISA], radioimmunoassay [RIA], antibody-dependent cell-mediated cytotoxicity [ADCC]) were compared during labial infection of BALB/c mice with herpes simplex virus type 1 strain Patton. The ELISA/RIA antibody responses were present in most mice by day 5 after infection, at the beginning of the herpetic lip lesions; antibody effective in ADCC showed identical early kinetics. In contrast, NT/CF antibodies were not detected in most mice until day 10, at the time of resolution of the herpetic lip lesions. The humoral immune responses persisted for at least 6 months after infection. The NT and CF responses were closely correlated in time of appearance and titers (r = 0.9), as were the ELISA and RIA responses (r = 0.99). However, there was little correlation between NT/CF and ELISA/RIA responses (r = 0.02). The kinetics of the delayed type hypersensitivity response showed similar kinetics of appearance to the ELISA/RIA/ADCC humoral responses, and peaked similarly, but waned gradually over 2 months. The importance of antibody in protection against labial herpes simplex virus type 1 infection was demonstrated by the ability of passively transferred convalescent serum (that produced a minimum NT titer of 10 in recipient mice) to protect against development of herpetic lesions and death.

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Section: Differences Between Nt and Ria Antibodiesmentioning

confidence: 85%

Immune responses to labial infection of BALB/c mice with herpes simplex virus type 1

Morahan¹,

Thomson²,

Kohl³

et al. 1981

Infect Immun

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“…Latent virus infection is characterized by a relapse after the continuous persistence of the agent, and R. prowazekii is equivalent to viruses in this respect. As in herpes simplex virus infection, differences in the relative proportions of virusneutralizing and virus-nonneutralizing antibodies in human sera were found and were related to the recurrence of oral herpetic lesions (11,24). Sera with virus neutralizing activity had higher antibody titers to structural components of the virus, as determined by radioimmunoassay (24).…”

Section: Resultsmentioning

confidence: 99%

“…As in herpes simplex virus infection, differences in the relative proportions of virusneutralizing and virus-nonneutralizing antibodies in human sera were found and were related to the recurrence of oral herpetic lesions (11,24). Sera with virus neutralizing activity had higher antibody titers to structural components of the virus, as determined by radioimmunoassay (24). Different levels of response to various viral antigens were also revealed by immunoblotting techniques, but no differences between the recognition of viral polypeptides by sera from patients with various frequencies of herpesvirus infections (11) have been observed in comparison with that by sera from patients with primary infection (2).…”

Section: Resultsmentioning

confidence: 99%

Serological response of patients suffering from primary and recrudescent typhus: comparison of complement fixation reaction, Weil-Felix test, microimmunofluorescence, and immunoblotting

Eremeeva

Balayeva

Raoult

1994

Clin Diagn Lab Immunol

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Microimmunofluorescence and Western immunoblotting were compared with the classical complement fixation reaction and the Weil-Felix test to study the serological responses of patients to Rickettsia prowazekii and both Proteus vulgaris OX19 and OX2 during primary and recrudescent typhus infections. The serological response to R. prowazekii was found to be similar during primary and recrudescent typhus, and all sera examined contained antibodies to the same R. prowazekii cell structures. Immunoglobulin G (IgG) and IgM were found to be the dominant anti-R. prowazekii immunoglobulins in all sera tested and were found to be directed against the 100-kDa protein and the lipopolysaccharide. IgA antibodies, when present, were mainly against the 100-kDa protein. For P. vulgaris, IgG antibodies recognized the proteins and lipopolysaccharides of both OX19 and OX2 serotypes; IgM antibodies were directed against the P. vulgaris OX2 lipopolysaccharide. In addition, donor blood sera, which were negative by microimmunofluorescence, were found to contain IgG immunoglobulins reacting with R. prowazekii protein antigens of 135, 60, and 47 kDa by western immunoblotting.

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“…ELISA or RIA titers measured against HSV gD are consistently much higher because they measure antibodies that bind antigen in a non-neutralizing manner as well as antibodies that could neutralize [ 4 – 6 , 27 , 31 ]. However, a strong correlation exists between ELISA or RIA titers and neutralizing activity whether considering individual subject sera [ 4 , 31 , 32 ] or fold increase in peak titers of subject groups after successive vaccine doses [ 1 , 6 ]. Conclusions reached using ELISA occasionally are inconsistent with assays of neutralizing ability when comparing titers against gD1 versus gD2.…”

Section: Discussionmentioning

confidence: 99%

Higher Throughput Quantification of Neutralizing Antibody to Herpes Simplex Viruses

et al. 2015

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We report a rapid, higher throughput method for measuring neutralizing antibody to herpes simplex virus (HSV) in human sera. Clinical isolates and sera from the Herpevac Trial for Women were used in a colorimetric assay in which infection of tissue culture (lack of neutralization) was indicated by substrate metabolism by beta-galactosidase induced in the ELVIS cell line. The neutralization assay was optimized by addition of guinea pig complement, which particularly enhanced neutralizing antibody titers to HSV-2. Higher neutralizing antibody titers were also achieved using virus particles isolated from the supernatant of infected cells rather than lysate of infected cells as the source of virus. The effect of assay incubation time and incubation time with substrate were also optimized. We found that incubating with substrate until a standard optical density of 1.0 was reached permitted a better comparison among virus isolates, and achieved reliable measurement of neutralizing antibody activity. Interestingly, in contrast to results in the absence of complement, addition of complement allowed sera from HSV-2 gD-vaccinated subjects to neutralize HSV-1 and HSV-2 clinical and laboratory isolates with equal potency.

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Serum antibodies to herpes simplex virus type 1 during active oral herpes infection

Cited by 10 publications

References 14 publications

Immune responses to labial infection of BALB/c mice with herpes simplex virus type 1

Immune responses to labial infection of BALB/c mice with herpes simplex virus type 1

Serological response of patients suffering from primary and recrudescent typhus: comparison of complement fixation reaction, Weil-Felix test, microimmunofluorescence, and immunoblotting

Higher Throughput Quantification of Neutralizing Antibody to Herpes Simplex Viruses

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