Serum and Urinary Folate in Liver Disease

Retief, François; Huskisson, Y. J.

doi:10.1136/bmj.2.5650.150

Cited by 30 publications

(9 citation statements)

References 17 publications

(10 reference statements)

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“…'-Discussion These studies confirmed our previous findings (Retief and Huskisson, 1969) that serum folate consists of a dialyzable and non-dialyzable fraction. The non-dialyzable fraction is probably protein bound, eluting from the Sephadex G-25 column in association with the serum proteins.…”

Section: Methodssupporting

confidence: 91%

“…We previously reported a non-dialyzable folate fraction present in serum but absent from urine (Retief and Huskisson, 1969). In the present study this non-dialyzable, and possibly proteinbound, folate fraction was further investigated.…”

mentioning

confidence: 74%

“…Aliquots, each of 1 to 2 ml, of urine, serum, saliva, gastric juice, bile, and haemolyzed normal whole blood (1 part blood: 9 parts distilled water) were dialyzed in Visking casing against 0-9% NaCl in the dark at 4°C for 48 hours (Retief and Huskisson, 1969). Folate levels measured before and after dialysis were referred to as 'total folate' and 'bound folate', respectively.…”

Section: Methodsmentioning

confidence: 99%

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Folate binders in body fluids

Retief

Huskisson

1970

J Clin Pathol

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SYNOPSIS In normal serum, saline dialysis for 48 hours in Visking casing resulted in folate clearance closely comparable to that produced by haemoglobin-coated charcoal adsorption, except in kwashiorkor where charcoal removed a greater proportion of folate. Pre-and post-dialysis values probably represented total and bound folate, respectively. Urinary folate consisted almost exclusively of dialyzable or free folate. Folate in saliva, bile, and erythrocytes consisted of dialyzable and non-dialyzable fractions; gastric juice contained minimal amounts of folate.

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Section: Methodssupporting

confidence: 91%

mentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

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Folate binders in body fluids

Retief

Huskisson

1970

J Clin Pathol

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“…On the other hand, the FAA attached to protein fractions seems to be firmly bound, since it tolerates, e.g., an ammonium sulphate precipitation and repeated gel filtrations without abandoning its attachment to the corresponding proteins [unpublished data], FAA recovery in these cases was nearly 100%. Similar results concerning the occurrence of FAA in-and outside the protein band have been reported by Retief and H uskisson [6,7], Their absorption studies with charcoal pre parations coated with molecules of varying size suggested that the main serum folate binder is of molecular weight 70,000-120,000. Since the mole cular weight of albumin is 69,000, it seems probable that the conclusion of these authors concerning the FAA-carrying protein agrees with our finding.…”

Section: Discussionsupporting

confidence: 74%

Carrier Proteins of Folic Acid Activity in Human Serum

Markkanen¹,

Peltola²

1971

Acta Haematol

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The natural folic acid activity (FAA) in the serum, on Sephadex® G-200 gel filtration, is divided into 3 fractions: the first in the band of largemolecular proteins; the second in that of small-molecular proteins; and the third outside the protein band. The second fraction, in the present study, was purified by hydroxyapatite chromatography, with the result that FAA emerged over a narrow area of fractions. In immunoelectrophoresis anddisc electrophoresis this area contained only albumin

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“…The majority of these coincided with albumin. In addition, H ampers et al [4], Sevitt and H offbrand [13], and R etief and H uskisson [12] have discussed the question of the binding of FAA in their papers on renal and hepatic diseases. M etz and H erbert [10] mentioned unsuccessful attempts to demonstrate binders for folic acid in normal human serum, analogous to those present for vitamin B12.…”

mentioning

confidence: 99%