Folate binders in body fluids

Retief, François; Huskisson, Y. J.

doi:10.1136/jcp.23.8.703

Cited by 25 publications

(24 citation statements)

References 10 publications

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“…On the other hand, the FAA attached to protein fractions seems to be firmly bound, since it tolerates, e.g., an ammonium sulphate precipitation and repeated gel filtrations without abandoning its attachment to the corresponding proteins [unpublished data], FAA recovery in these cases was nearly 100%. Similar results concerning the occurrence of FAA in-and outside the protein band have been reported by Retief and H uskisson [6,7], Their absorption studies with charcoal pre parations coated with molecules of varying size suggested that the main serum folate binder is of molecular weight 70,000-120,000. Since the mole cular weight of albumin is 69,000, it seems probable that the conclusion of these authors concerning the FAA-carrying protein agrees with our finding.…”

Section: Discussionsupporting

confidence: 75%

Carrier Proteins of Folic Acid Activity in Human Serum

Markkanen¹,

Peltola²

1971

Acta Haematol

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The natural folic acid activity (FAA) in the serum, on Sephadex® G-200 gel filtration, is divided into 3 fractions: the first in the band of largemolecular proteins; the second in that of small-molecular proteins; and the third outside the protein band. The second fraction, in the present study, was purified by hydroxyapatite chromatography, with the result that FAA emerged over a narrow area of fractions. In immunoelectrophoresis anddisc electrophoresis this area contained only albumin

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Section: Discussionsupporting

confidence: 75%

Carrier Proteins of Folic Acid Activity in Human Serum

Markkanen¹,

Peltola²

1971

Acta Haematol

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“…Normal human plasma contains 6 to 20 ng of FA per ml (24), most of which is nonspecifically protein bound and freely dialyzable (16). The PABA concentration of normal human plasma varies greatly but is generally less than 1.0 ng/ml (R. Desjardins, unpublished observations).…”

mentioning

confidence: 99%

In vitro activities of and mechanisms of resistance to antifol antimalarial drugs

Milhous¹,

Weatherly²,

Bowdre³

et al. 1985

Antimicrob Agents Chemother

199

148

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Certain drugs that interfere with folate metabolism (sulfones, sulfonamides, and inhibitors of dihydrofolate reductase) play an important role in the chemotherapy and prophylaxis of malaria. The activities and mechanisms of action of these drugs are regarded as similar in most respects to their activities against procaryotic microorganisms. Believed incapable of utilizing intact exogenous folates, plasmodia have been regarded as dependent on de novo synthesis of required folate cofactors. The present investigation, conducted in pursuit of a method for testing the in vitro susceptibility of Plasmodium falciparum to antifol antimalarial drugs, produced evidence that earlier assumptions about the folate metabolism of this organism are not correct. Three of four isolates of P. falciparum were successfully maintained in a culture medium depleted of folic acid and p-aminobenzoic acid. The antimalarial activities of sulfonamides and dihydrofolate reductase inhibitors were, furthermore, variably antagonized by the presence of folic acid and p-aminobenzoic acid in the culture medium. Optimum conditions for assessment of antifol antimalarial activity in vitro therefore require precise control of these factors in the culture medium. Our results suggest that resistance to antifol antimalarial drugs involves a complex of factors related to both the de novo synthesis of active folate cofactors and the ability to utilize exogenous intact folates in various forms.

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“…It has been suggested that this might be due to hormonally in duced synthesis of folate binder as it also occurred in women taking oral contra ceptives (6). As mentioned earlier, serum folate decreased during pregnancy and as the urinary folate excretion was higher in pregnant than in non-pregnant women, it was therefore postulated that urinary loss of folate could be one factor contri buting to the fall of serum folate activity (13)(14)(15). In order to compensate for the loss of high urinary folate and the greater demand of folate during pregnancy, the body therefore increased synthesis of serum FABP for binding the required folate.…”

Section: Discussionmentioning

confidence: 99%

Folic acid and folate binding protein in pregnancy.

Areekul

Yamarat

Vongyuthithum

1977

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryFolic acid and folate binding protein (FABP) concentrations were determined in 208 pregnant women as well as in 50 non-pregnant women. Serum folate levels in pregnant women were significantly lower than those of the non-pregnant group. Anaemic pregnant women also had lower serum folate concentrations than those of the non-anaemic pregnant women. Ninetyeight of 208 (47.1%) pregnant women had serum folate levels of less than 4ng/ml. No significant dierence in red cell folate concentration between the pregnant and non-pregnant groups was demonstrated, and only 5 of 206 (2.4%) of pregnant women had red cell folate levels of less than 200ng/ml. Serum FABP levels in preg nant women were also found to be significantly higher (p<0.001) than those of the non-pregnant women. These levels were also found to be higher in the anaemic pregnant group and in pregnant women with serum folate concentration less than 4ng/ml than those of the non-anaemic pregnant women and pregnant women with serum folate concentration higher than 4ng/ml, respectively.There was a direct relationship between the serum FABP levels and the gestation period.As the period of pregnancy advanced higher serum FABP levels were demonstrated.No correlation between the gravida and the serum FABP levels was found in this study.

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Folate binders in body fluids

Cited by 25 publications

References 10 publications

Carrier Proteins of Folic Acid Activity in Human Serum

Carrier Proteins of Folic Acid Activity in Human Serum

In vitro activities of and mechanisms of resistance to antifol antimalarial drugs

Folic acid and folate binding protein in pregnancy.

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