Serum and skin lysozyme activity in several skin disorders

Papini, Manuela; Simonetti, S.; Franceschini, S. do C. C.; Binazzi, M

doi:10.1007/bf00516559

Cited by 3 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Klenha and Krs [ 4 ] have shown the presence of lysozyme on human’s skin surface at the amount of 5 × 10 -3 µg/cm 2 . Papini et al [ 5 ] have demonstrated a consistent decrease in lysozyme activity in healthy skin in the untreated diabetic patients. They also found that the presence of lichen planus, psoriasis, conglobate acne, and chronic tuberculoid cutaneous toxoplasmosis did not affect serum and skin lysozyme levels in either normoglycemic or diabetic patients.…”

Section: Introductionmentioning

confidence: 98%

Regulation of Lysozyme Activity by Human Hormones

Pankratov

Ганнесен

Nikolaev

2023

IBJ

View full text Add to dashboard Cite

Background: Lysozyme is a part of human and animal noncellular immunity. The regulation of its activity by hormones is poorly studied. The aim of this study was to test the in vitro activity of lysozyme in the presence of catecholamines, natriuretic hormones, and E2. Methods: Hormones were incubated with lysozyme, and the activity of lysozome was further determined using a test culture of Micrococcus luteus in the early exponential growth stage. The activity of lysozyme was assessed based on the rate of change in the OD of the test culture. Molecular docking was performed using SwissDock server (http://www.swissdock.ch/docking), and molecular structures were further analyzed and visualized in the UCSF Chimera 1.15rc software. Results: According to the results, EPN and NE increased lysozyme activity up to 180% compared to the hormone-free enzyme. Changing the pH of the medium from 6.3 to 5.5, increased the lysozyme activity in the presence of E2 up to 150-200 %. The results also showed that exposure to hormones could modify lysozyme activity, and this effect depends on the temperature and pH value. The molecular docking revealed a decrease in the activation energy of the active site of enzyme during the interaction of catecholamines with the amino acid residues, asp52 and glu35 of the active site. Conclusion: Our findings demonstrate an additional mechanism for the involvement of lysozyme in humoral regulation of nonspecific immunity with respect to human pathogenic microflora and bacterial skin commensals by direct modulation of its activity using human hormones.

show abstract

Section: Introductionmentioning

confidence: 98%

Regulation of Lysozyme Activity by Human Hormones

Pankratov

Ганнесен

Nikolaev

2023

IBJ

View full text Add to dashboard Cite

show abstract

Antimicrobial peptides and proteins in human biological fluids

Iksanova

Arzumanyan

Konanykhina

et al. 2022

Microbiology Independent Research Journal (MIR Journal)

View full text Add to dashboard Cite

Antimicrobial peptides and proteins (AMPs) are endogenous compounds that have a direct antimicrobial effect on bacteria (e. g., by disrupting bacterial membranes), as well as on fungi and viruses. AMPs are the main component of the innate immunity of living organisms and are produced by both epithelial cells (skin cells, cells of respiratory tract, intestine, urinary and genital tracts) and cells of the immune system and are secreted into secretory fluids. AMPs can also act as chemoattractants for immunocompetent cells (neutrophils, monocytes, T lymphocytes, dendritic cells) in the inflammation site and affect the antigen presenting cells by modulating adaptive T cell immune responses. The representatives of the main 15 AMP classes, that we describe in this review, are the most studied group of the large pool of these compounds. We discuss their localization, expression, and concentration in various biofluids of humans under normal and pathological conditions.

show abstract

Антимикробные Пептиды И Белки В Биожидкостях Человека

Iksanova¹,

Arzumanyan²,

Konanykhina³

et al. 2022

Microbiology Independent Research Journal (MIR Journal)

View full text Add to dashboard Cite

Антимикробные пептиды и белки (АМПБ) – это эндогенные соединения, оказывающие прямое противомикробное действие на бактерии, а также на грибы и вирусы. АМПБ являются основным компонентом врожденного иммунитета живых организмов и вырабатываются как эпителиальными клетками (клетки кожи, дыхательных путей, кишечника, мочевыводящих и половых путей), так и клетками иммунной системы, и выделяются в секреторные жидкости. Помимо антимикробной функции АМПБ могут выполнять роль хемоаттрактантов иммунокомпетентных клеток (нейтрофилы, моноциты, Т лимфоциты, дендритные клетки) в очаг воспаления. АМПБ оказывают влияние также на антигенпрезентирующие клетки, модулируя адаптивные T-клеточные иммунные ответы. Из большого количества описанных АМПБ наиболее изученными являются 15 основных классов, которые мы рассматриваем в данном обзоре, включая их локализацию, экспрессию и концентрацию в различных биожидкостях в норме и при патологии.

show abstract

Serum and skin lysozyme activity in several skin disorders

Cited by 3 publications

References 2 publications

Regulation of Lysozyme Activity by Human Hormones

Regulation of Lysozyme Activity by Human Hormones

Antimicrobial peptides and proteins in human biological fluids

Антимикробные Пептиды И Белки В Биожидкостях Человека

Contact Info

Product

Resources

About