Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer

Khanzode, Shruti; Khanzode, Suchet D.; Dakhale, Ganesh N.

doi:10.1016/s0304-3835(03)00147-2

Cited by 49 publications

(54 citation statements)

References 17 publications

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“…One of the important mechanisms of H. pylori gastritis is the production of reactive oxygen species (ROS) and lipid peroxidation products accumulation, such as malondialdehyde (MDA). These oxidative products feature genotoxic and carcinogenic properties (13) . Several studies have shown that the changes and damages of oxidative deoxyribonucleic acid (DNA) associated with H. pylori gastritis as well as stomach cancer are due to the imbalance between endogenous antioxidants, such as superoxide dismutase (SOD), Glutathione peroxidase (GPx), catalase (CAT), and ascorbic acid and lipid peroxidation product accumulation in the cells (7,26) .…”

Section: Introductionmentioning

confidence: 99%

Curcumin in Combination With Triple Therapy Regimes Ameliorates Oxidative Stress and Histopathologic Changes in Chronic Gastritis-Associated Helicobacter Pylori Infection

Judaki

Rahmani

Feizi

et al. 2017

Arq. Gastroenterol.

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-Background -Helicobacter pylori (H. pylori) gastric infection is a main cause of inflammatory changes and gastric cancers. Objective -The aim of this study was finding the effects of curcumin on oxidative stress and histological changes in chronic gastritis associated with H. pylori. Me thods -In a randomized clinical trial, patients were divided into two groups: a standard triple therapy group and triple therapy with curcumin group. Endoscopic and histological examinations were measured for all patients before and after 8 weeks. Results -Triple therapy with curcumin treatment group significantly decreased malondialdehyde markers, glutathione peroxides and increased total antioxidant capacity of the gastric mucosa at the end of study compared to baseline and triple regimen groups. In addition, the oxidative damage to DNA was significantly decreased in triple therapy with curcumin group at the end of study compared to baseline and compared to triple therapy (P<0.05 for both). Triple therapy group in combination with Curcumin significantly decreased all active, chronic and endoscopic inflammation scores of patients compared to the baseline and triple therapy group (P<0.05 for both). The eradication rate by triple therapy + curcumin was significantly increased compared to triple therapy alone (P<0.05). Conclusion -Curcumin can be a useful supplement to improve chronic inflammation and prevention of carcinogenic changes in patients with chronic gastritis associated by H. pylori. HEADINGS -Gastritis. Helicobacter pylori. Curcumin. Oxidative stress. DNA damage.Declared conflict of interest of all authors: none Disclosure of funding: no funding received

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Section: Introductionmentioning

confidence: 99%

Curcumin in Combination With Triple Therapy Regimes Ameliorates Oxidative Stress and Histopathologic Changes in Chronic Gastritis-Associated Helicobacter Pylori Infection

Judaki

Rahmani

Feizi

et al. 2017

Arq. Gastroenterol.

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“…With the advent of appropriate analysis techniques, measures of vitamin C in biological samples have been used as a biomarker of health status [e.g., in the study of gastric cancer (4,5) and total mortality (6)]. Because vitamin C in biological samples can easily degrade or be readily oxidized, it is often stabilized before sample storage by the addition of stabilizing agents such as EDTA (a metal chelator), perchloric acid, DTT, or metaphosphoric acid, all of which act as protein-precipitating agents (7).…”

Section: Introductionmentioning

confidence: 99%

Long-term Cryoconservation and Stability of Vitamin C in Serum Samples of the European Prospective Investigation into Cancer and Nutrition

Jenab¹,

Bingham

Ferrari

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Plasma vitamin C level may be associated with risk of some chronic diseases. The rapid degradability of vitamin C in biological samples necessitates its stabilization with metaphosphoric acid or similar agents. However, in most cohort studies, prospectively collected biological samples are not treated with stabilizing agents before long-term frozen storage and it is not known whether vitamin C can be properly measured in such samples. The objective of this study was to determine the degree of vitamin C degradation in plasma samples stored without stabilization for 7 to 11 years at À196°C. Spearman's correlation coefficients indicate a moderate correlation between baseline and final plasma vitamin C levels in both men (r = 0.57, P < 0.0001) and women (r = 0.52, P < 0.0001). Samples were also categorized based on low or high baseline levels of plasma vitamin C, with the latter category showing the highest rate of loss per year of frozen storage in men (1.96 Mmol/L, P value for difference <0.0001; percent loss 24.6%) and women (2.35 Mmol/L, P value for difference <0.0001; percent loss 24.2%), as determined by multiple regression analysis adjusted for smoking status, age, and body mass index. In men, both baseline and final plasma vitamin C values were lower in smokers than never smokers, but for both men and women the rate of vitamin C loss during storage was not significantly different between smokers and never smokers. The results of this study show that vitamin C can be measured with reasonable reliability in plasma samples frozen for long periods of time without addition of any stabilizing agents. (Cancer Epidemiol Biomarkers Prev 2005;14(7):1837 -40)

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“…For measurement of GSSG, 30 μL of scavenger from GSSG kit was added to 100 µl of serum and then all serum samples were kept at -80°C until they were tested. MDA levels in samples were spectrophotometrically determined (Khanzode et al, 2003).…”

Section: Helicobacter Pylori Pcr Detectionmentioning

confidence: 99%

Effect of Helicobacter pylori infection on oxidative stresses in patients with chronic gastritis

Navvabi¹,

Ansari²,

Navvabi³

et al. 2013

Afr. J. Microbiol. Res.

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The Helicobacter pylori infection is currently endemic worldwide with high prevalence in developing regions. The infection causes chronic gastritis, gastric and gastric adenoid carcinoma. Infection by H. pylori may act as a risk factor for proteins, lipids and DNA damages. In this study, gastric biopsies were obtained in patients with a chronic gastric to investigate effects of H. pylori infection on oxidative stress in patients with chronic gastritis. Urease test and polymerase chain reaction (PCR) were performed on endoscopic gastric biopsy obtained from 150 subjects. The result showed that the presence of a 492-bp DNA fragment was indicative of a positive reaction, also fresh samples had a positive urease test for H. pylori. Malondialdehyde (MDA) in patients with gastritis as a result of H. pylori were significantly increased as compared to the control groups (p<0.05). Mean ± SE of reduced glutathione (GSH) and total antioxidant capacity (TAC) in patients were decreased significantly (p<0.05). The result shows mean ± SE of oxidized glutathione (GSSG) in patients showed significant increased as compared to the control groups. These finding show that H. pylori infection can cause oxidative stress disorders that may act as risk factors.

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Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer

Cited by 49 publications

References 17 publications

Curcumin in Combination With Triple Therapy Regimes Ameliorates Oxidative Stress and Histopathologic Changes in Chronic Gastritis-Associated Helicobacter Pylori Infection

Curcumin in Combination With Triple Therapy Regimes Ameliorates Oxidative Stress and Histopathologic Changes in Chronic Gastritis-Associated Helicobacter Pylori Infection

Long-term Cryoconservation and Stability of Vitamin C in Serum Samples of the European Prospective Investigation into Cancer and Nutrition

Effect of Helicobacter pylori infection on oxidative stresses in patients with chronic gastritis

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