Serum and cerebrospinal fluid Neudesin concentration and Neudesin Quotient as potential circulating biomarkers of a primary brain tumor

Abstract

Background Despite the previously suggested role of Neudesin in tumorigenesis and its potential as a novel target for the treatment of cancers, its prognostic value has never been examined. Thus, the aim of the study was to evaluate Neudesin concentrations in primary brain tumor patients and make a comparison with non-tumoral individuals. Methods Cerebrospinal fluid (CSF) and serum Neudesin concentration was evaluated by means of the ELISA method. Results The total group of brain tumor patients had statistically lower serum Neudesin concentrations compared to the non-tumoral group ( P = 0.037). The meningeal tumor subgroup also had statistically lower serum Neudesin concentrations compared to the non-tumoral group ( P = 0.012). The Astrocytic brain tumor subgroup had significantly higher CSF Neudesin concentrations compared to the non-tumoral group ( P = 0.046). Neudesin Quotient (CSF concentration divided by serum concentration) in the astrocytic brain tumor subgroup was statistically higher compared to the non-tumoral group ( P = 0.023). Males had statistically lower concentrations of the serum Neudesin compared to females ( P = 0.047). Univariate linear regression analysis revealed that for women the serum Neudesin concentration was 1.53 times higher than for men. In the model of multivariate linear regression analysis, predictor variables influencing serum Neudesin concentrations included CSF Neudesin concentration and the Neudesin Quotient, if other model parameters are fixed. The developed model explains 82% of the variance in serum Neudesin concentration. Both linear regression models, univariate and multivariate, pointed to fewer factors with a potential to influence the Neudesin Quotient compared to serum Neudesin concentration. Conclusions In astrocytic brain tumor patients Neudesin concentrations within the cerebrospinal fluid are higher compared with non-tumoral individuals. Serum Neudesin concentration strongly correlates with its CSF level. In primary brain tumor patients serum Neudesin concentration is clearly gender-dependent. Linear regression models pointed to fewer factors that may influence the Neudesin Quotient value, which suggests it is a better biomarker of astrocytic brain tumors than serum and CSF Neudesin concentrations alone.

Intraoperative Peritoneal Interleukin-6 Concentration Changes in Relation to the High-Mobility Group Protein B1 and Heat Shock Protein 70 Levels in Children Undergoing Cholecystectomy

“…The concentrations of high-mobility group protein B1 (HMGB1) and heat shock protein 70 (HSP70) in peritoneal lavage fluid samples were analyzed with the use of an ELISA (enzyme-linked immunosorbent assay) method, as described elsewhere [ 34 ].…”

Ratio of IL-8 in CSF Versus Serum Is Elevated in Patients with Unruptured Brain Aneurysm

“…To calculate IL-8 and MCP-1 Quotients, we divided CSF chemokine result by its concentration in serum, as described elsewhere [30][31][32]. Such an approach allowed to exclude possible influence of the blood-CSF barrier impairment and/or blood-brain barrier functions on IL-8 and MCP-1 levels.…”

“…Taking into consideration the above, the aim of the study was the evaluation of CSF and serum IL-8 and MCP-1 concentration in patients with unruptured intracranial aneurysm. To exclude influence of the blood-brain barrier and the blood-CSF barrier on mutual relationships of protein levels in these two compartments [30][31][32], we also calculated IL-8 and MCP-1 Quotients by referring their CSF values to the serum values. In our study we tested if the evaluation of IL-8 and MCP-1 concentration would allow to diagnose brain aneurysm before its rupture.…”

Plasma Levels of Neudesin and Glucose Metabolism in Obese and Overweight Children

“…In neudesin gene knockout mice, increased energy expenditure, lipolysis in white adipose tissue, heat production of brown adipose tissue, and a protection to IR related to high-fat diet and decreased food intake were shown (16); these data have thus led to focus attention on the action of neudesin as a possible negative regulator of energy expenditure. However, few and discordant studies are available in the literature on the levels of neudesin in biological fluids of patients (17)(18)(19)(20)(21)(22)(23) and no data on childhood obesity are yet available.…”