Serum amyloid A (SAA) mRNA expression in chicken and quails in response to bacterial stress

Singh, Prabhjot; Singh, Sanjeev; Kumar, B. V. Sunil; Sethi, R. S.; Verma, Ramneek

doi:10.1016/j.cimid.2020.101421

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Our results revealed that SE challenge significantly (p < 0.05) upregulated expression of serum amyloid A (SAA) in the cecum, and dietary supplementation of Spirulina suppresses this expression at 3-day PI as compared to the challenged treated group (G4). Similarly, Singh et al [42] reported that expression of the SAA gene in quails challenged with Salmonella was significantly upregulated than control groups.…”

Section: Discussionmentioning

confidence: 90%

“…Dietary Spirulina supplementation significantly decreased the level of MDA values and increased activity of SOD and GPX in both non challenged treated groups (G2 and G3) and in challenged treated groups (G5 and G6) as compared to the control negative group (G1) and control positive group (G4) respectively (Table 7). There was a significant increase (p < 0.05) in the level of SOD and GPX G. Abd El-Dayem et al 2021/ Effect of Spirulina platensis in quail Mans Vet Med J 22:2 (2021) [38][39][40][41][42][43][44][45][46][47] with an increased dietary level of spirulina inclusion from (1-2 g).…”

Section: Serum Antioxidant Statusmentioning

confidence: 99%

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Impact of dietary Spirulina (arthrospira) platensis on growth performance, gene expression and antioxidant status of quail challenged with salmonella enteritidis.

EL-Dayem

Saleh

Elwahab³

2021

Mansoura Veterinary Medical Journal

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To investigate the effect of dietary Spirulina platensis supplementation on growth performance, organ colonization, expression of inflammatory-related genes, and antioxidant status in quail challenged with Salmonella enteritidis. Design: Randomized controlled experimental study. Animals: hundred eighty-seven-day-old Japanese quail. Procedures: birds were randomly allocated into 6 equal groups. G1, G2, and G3: non challenged and fed abasal diet supplemented with Spirulina at 0, 1, and 2 g/kg diet, respectively. G4, G5, and G6: challenged and fed a basal diet supplemented with Spirulina at 0, 1, and 2 g/kg diet, respectively. At 21 days of age, all challenged groups were orally inoculated with 1ml of (1.00x10 7 Salmonella enteritidis/ml). The collected samples were serum for determining biochemical and antioxidant parameters, cecal tissue samples for determination of gene expression of inflammatory-related genes, and tissue samples from liver, heart, spleen, and caecum for bacterial reisolation. Results: The dietary supplementation of Spirulina significantly improved growth performance parameters and reduced organ colonization. The cecal pro-inflammatory gene expressions (IL-6, IL1 ß and TNFα) were significantly downregulated while the anti-inflammatory cytokine (IL-10) was significantly increased. In addition, gene expression of cecal serum amyloid (SAA) was significantly down-regulated. The antioxidant and serum biochemical parameters were improved. Conclusion and clinical relevance: dietary supplementation of Spirulina could be a helpful strategy for mitigating the harmful effects of Salmonella enteritidis in quail.

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Section: Discussionmentioning

confidence: 90%

Section: Serum Antioxidant Statusmentioning

confidence: 99%

Impact of dietary Spirulina (arthrospira) platensis on growth performance, gene expression and antioxidant status of quail challenged with salmonella enteritidis.

EL-Dayem

Saleh

Elwahab³

2021

Mansoura Veterinary Medical Journal

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“…In particular, C-reactive protein (CRP) in dogs and serum amyloid A (SAA) in cats have been routinely tested in small animal clinics as measurement kits have been commercialized. Known avian APPs include SAA, alpha-1 acid glycoprotein, ceruloplasmin, transferrin, fibrinogen, and haptoglobin (8)(9)(10)(11)(12)(13)(14)(15). Among these avian APPs, SAA has been the focus of research because it is a precursor of the amyloid A (AA) protein.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of serum amyloid A in differentiating the inflammatory disorders in wild birds

Rhim,

Kim,

Gim

et al. 2024

Front. Vet. Sci.

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Rescued wild birds have very high rates of inflammatory diseases; however, there have been limitations in assessing them sensitively. Few studies have examined acute-phase proteins in wild birds. In this study, serum amyloid A (SAA) was evaluated as an inflammatory indicator along with traditional indices such as white blood cell count, albumin, and albumin/globulin ratio. In total, 291 samples from 139 birds of six avian species were analyzed. All samples were divided into four groups (severe, moderate, mild injuries, and clinically healthy) based on clinical examinations and evaluated by group. SAA levels were measured using an anti-chicken SAA ELISA kit and compared with leukocyte counts, albumin concentrations, and albumin/globulin ratios. Differences among groups were evaluated using the Kruskal–Wallis test, followed by a post hoc test using Dunn’s multiple comparisons with SPSS V27. Statistical significance was set at a value of p of <0.05. The median concentration and interquartile range (ng/mL) of SAA in each group were 78.10 ng/mL (50.71–109.30), 31.15 ng/mL (19.85–49.24), 9.68 ng/mL (4.71–22.06), and 3.10 ng/mL (1.50–6.00). We observed a significant difference in the mean levels between the groups (p < 0.001), with the same results observed across species. All four indices showed significant differences in mean values between the groups (p < 0.001). In addition, SAA showed rapid changes in periodically collected samples, reflecting either a positive treatment response or the onset of subclinical diseases. SAA can be used to detect inflammatory conditions and asymptomatic disease in wild birds and is helpful in accurately identifying current health status, which is essential for successful treatment and release.

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Soluble components and acute-phase proteins

Veldhuizen

Dalgaard

2022

Avian Immunology

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Serum amyloid A (SAA) mRNA expression in chicken and quails in response to bacterial stress

Cited by 5 publications

References 12 publications

Impact of dietary Spirulina (arthrospira) platensis on growth performance, gene expression and antioxidant status of quail challenged with salmonella enteritidis.

Impact of dietary Spirulina (arthrospira) platensis on growth performance, gene expression and antioxidant status of quail challenged with salmonella enteritidis.

Diagnostic value of serum amyloid A in differentiating the inflammatory disorders in wild birds

Soluble components and acute-phase proteins

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