Serum agglutination test for swine dysentery

Hunter, D; Saunders, CN

doi:10.1136/vr.93.4.107-a

Cited by 17 publications

(11 citation statements)

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“…Workers in England [19] reported using a serum agglutination test for swine dysentery. Their antigen was prepared from a strain of spirochetes known to be pathogenic for pigs.…”

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confidence: 99%

A Study of Swine Dysentery by Immunofluorescence and Histology

et al. 1977

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Abstract. Twenty-six specific-pathogen-free pigs were fed pure cultures of Treponemu hyodysenteriue. Five untreated pigs were controls. Distribution of this large spirochete in pigs with swine dysentery was shown by the indirect fluorescent antibody technique. Findings by this method were compared with those from dark-field examination of colonic mucosal scrapings and from tissue sections. The cultures caused mucohemorrhagic colitis which by 10 days after inoculation was indistinguishable from the colitis of swine dysentery. Control pigs remained normal. Pigs killed when spirochetes were first seen in their feces had normal colonic mucosa with only a few spirochetes. At the first sign of diarrhea, however, the colonic mucosa was thicker than normal and had many spirochetes. T. hyodysenreriue was confined to regions of hypertrophy and exudation of the large intestine mucosa throughout the course of disease.Culture studies, dark-field microscopy, special staining techniques and electron microscopy have suggested a relationship between T. hyodysenreriue and swine dysentery [14][15][16]. Observational limitations, however, have precluded correlation of the findings with the development of the disease. Culture and dark-field observations place the organisms only in the gross vicinity of the lesion; special staining techniques do not identify organisms specifically, and electron microscopy gives a very selective view of the morphologic relationship between the organisms and the disease. We used specific immunofluorescent staining of T. hyodysenreriue in the affected tissues to correlate the presence of the organisms with the development of the lesions.A mucohemorrhagic diarrhea disease of pigs was shown to be distinct from hog cholera and salmonellosis in 1921 and was named swine dysentery [26]. A large spirochete was associated with the lesions but feeding a mixed culture containing the organism did not produce the disease. A comma-shaped organism also was seen in swine dysentery at that time and was later isolated and named Vibrio coli. This was considered the cause of the disease [9-111. Although V. coli was generally accepted to be the cause of swine dysentery [4,7, 10, 111, this organism has been commonly found in normal pigs [2, 8, 131, and in 490

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“…Workers in England [19] reported using a serum agglutination test for swine dysentery. Their antigen was prepared from a strain of spirochetes known to be pathogenic for pigs.…”

mentioning

confidence: 99%

A Study of Swine Dysentery by Immunofluorescence and Histology

et al. 1977

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“…Further development is required before it can be used to determine the swine dysentery status of a herd. Other serological methods of detecting recovered animals (Hunter and Saunders 1973) show little promise and there seems little reason for optimism concerning development of vaccines or other immunising agents (Kurtz andSorensen 1972, Hudson et a2 1974). Methods of prevention of swine dysentery must ultimately be based on clear knowledge of the factors which induce the disease in susceptible pigs.…”

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confidence: 99%

Aetiology and Pathogenesis of Swine Dysentery — Recent Advances

Callinan

Russell

1975

Aust Veterinary J

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“…These include plate agglutination and microtitration agglutination tests [6,7]. However, these tests are not effective in the detection of individual swine infections nor are they sensitive methods to evaluate post-infection antibody titres [8].…”

Section: Introductionmentioning

confidence: 99%

Application and evaluation of enzyme-linked immunosorbent assay and immunoblotting for detection of antibodies toTreponema hyodysenteriaein swine

Smith¹,

Barrett

Muir

et al. 1991

Epidemiol. Infect.

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SUMMARYAn enzyme-linked immunoassay (ELISA) has been developed to detect serum Immunoglobulin antibodies G and M to Treponema hyodysenteriae in vaccinated, experimentally infected and naturally infected swine. Naturally infected swine gave ELISA titres that were similar to experimentally infected swine, but were significantly less than the titres of vaccinated swine. When serum from naturally infected swine was used to probe nitrocellulose blots of sodium dodecyl sulphate-polyacrylamide gel electrophoresed whole cell proteins of T. hyodysenteriae, the immunoblotting patterns showed IgG antibodies were produced against many T. hyodysenteriae protein antigens and against lipopolysaccharide (LPS). The IgG antibodies directed against LPS were serotype-specific for that LPS and could be used to identify the serotype involved in the T. hyodysenteriae infection in that herd. IgM immunoblots also reacted with the many protein antigens but were less specific for LPS antigen, with a substantial degree of crossreaction between the LPS of all serotypes.The data demonstrate that a microplate enzyme-linked immunosorbent assay, coupled with immunoblotting, is a very specific and sensitive test for detection of antibody to Treponema hyodysenteriae in swine.

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Serum agglutination test for swine dysentery

Cited by 17 publications

References 0 publications

A Study of Swine Dysentery by Immunofluorescence and Histology

A Study of Swine Dysentery by Immunofluorescence and Histology

Aetiology and Pathogenesis of Swine Dysentery — Recent Advances

Application and evaluation of enzyme-linked immunosorbent assay and immunoblotting for detection of antibodies toTreponema hyodysenteriaein swine

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