Serum Adenosine Deaminase Level in Iraqi Women with Toxoplasmosis with a History of Abortion

Abstract

Sixty women who had abortion were selected for this study. Serum was collected from the women. ELISA test was used to detect antiToxoplasma specific antibodies. Those women were divided into three groups according to the presence or absence of specific anti-Toxoplasma antibodies. These groups included the women with IgG (18), the women with IgM (14) and the women with both IgG and IgM (12). The other 16 women had no antibodies against Toxoplasma. Twenty-four healthylooking women had been selected as controls. Serum was collected from those women and tested for anti-Toxoplasma antibodies. Those that revealed any antibody titer against Toxoplasma were excluded from the study. The activity of ADA was determined in the serum according to the method of Giusti. The mean concentration of ADA in the women who had anti-Toxoplasma IgM antibody was significantly higher (P<0.0001) than that of healthy controls and the women who had IgG, while there was no significant differences between women who had IgG antibody and healthy controls (P>0.05). Since a relationship exists between ADA activity and the cell mediated immune response, this result confirms the fact that Toxoplasma antigen induces T-lymphocytes proliferation.

A Correlation of Adenosine Deaminase Activity with Calcium Concentration in Sera of Renal Failure Patients

“…The reasons for these finding can be speculated that the stage of the disease and the immune response of the host may have a positive influence in the release of ADA from the tissue cells [7]. As a marker of cellular immunity, plasma ADA activity is found to be elevated in those diseases in which there is a cell-mediated immunity [13].…”

“…The activity was determined in the serum according to the method of Giusti with slight modification in order to prepare the reagents with volumes less than volumes which were mentioned in the reference [13]. All reagents were prepared from analar grade laboratory chemicals from the companies: BDH, Fluka, and Hopkins & williames.…”