SERS Nanotags and Their Applications in Biosensing and Bioimaging

Zhang, Wei; Jiang, Lianmei; Piper, James A.; Wang, Yuling

doi:10.1007/s41664-018-0053-9

Cited by 36 publications

(21 citation statements)

References 151 publications

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“…SERS has the ability to measure and detect single molecule while having multiplexing capabilities [ 46 ]. These features make SERS a powerful technique for sensitively and simultaneously detecting multiple biomarkers [ 41 , 47 , 48 ]. In this work, AuNPs and cetuximab-AuNPs with different sizes were synthesized and it was desired to study their cytotoxicity on BRAF-mutant HT-29 CRC cells (RAS wild-type) to advance the understanding of treatment response for cancers with BRAF mutation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

2021

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Epidermal growth factor receptor (EGFR) is estimated to be overexpressed in 60~80% of colorectal cancer (CRC), which is associated with a poor prognosis. Anti-EGFR targeted monoclonal antibodies (cetuximab and panitumumab) have played an important role in the treatment of metastatic CRC. However, the therapeutic response of anti-EGFR monoclonal antibodies is limited due to multiple resistance mechanisms. With the discovery of new functions for gold nanoparticles (AuNPs), we hypothesize that cetuximab-conjugated AuNPs (cetuximab-AuNPs) will not only improve the cytotoxicity for cancer cells, but also introduce expression change of the related biomarkers on cancer cell surface. In this contribution, we investigated the size-dependent cytotoxicity of cetuximab-AuNPs to CRC cell line (HT-29), while also monitored the expression of cell surface biomarkers in response to treatment with cetuximab and cetuximab-AuNPs. AuNPs with the size of 60 nm showed the highest impact for cell cytotoxicity, which was tested by cell counting kit-8 (CCK-8) assay. Three cell surface biomarkers including epithelial cell adhesion molecule (EpCAM), melanoma cell adhesion molecule (MCAM), and human epidermal growth factor receptor-3 (HER-3) were found to be expressed at higher heterogeneity when cetuximab was conjugated to AuNPs. Both surface-enhanced Raman scattering/spectroscopy (SERS) and flow cytometry demonstrated the correlation of cell surface biomarkers in response to the drug treatment. We thus believe this study provides powerful potential for drug-conjugated AuNPs to enhance cancer prognosis and therapy.

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Section: Introductionmentioning

confidence: 99%

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

2021

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“…For this reason, Surface Enhanced Raman Scattering (SERS) is a powerful vibrational spectroscopic technique that can extend the sensitivity of conventional Raman spectroscopy to the single molecule level [2][3][4]. The power of SERS can be exploited for its versatility and feasibility in a wide variety of fields including polymers, materials science, biochemistry and bio-sensing, catalyst, electrochemistry, pesticides, and food additives [5][6][7][8][9]. SERS technique increases drastically the Raman signals of an analyte molecule by the presence of roughened metal noble-metals (Ag and Au) near or in proximity of the analyzed samples [10,11].…”

Section: Introductionmentioning

confidence: 99%

SERS Activity of Silver Nanosphere, Triangular Nanoplates, Hexagonal Nanoplates and Quasi-Spherical Nanoparticles: Effect of Shape and Morphology

2020

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In this work, we prepared different morphologies of silver nanoparticles: nanosphere, triangular nanoplates, hexagonal nanoplates, and quasi-spherical shapes, through one-step synthesis. Hydrogen peroxide was used as the oxidizing agent during the reduction of silver nitrate by sodium borohydride, in the presence of tri-sodium citrate and poly-vinyl-pyrrolidone. The obtained silver nanoparticles were fully characterized by UV-Vis spectroscopy, Dynamic Light Scattering and Scanning Electron Microscopy, and successfully used as Surface Enhanced Raman Scattering (SERS) substrates. The effect of shape and morphology on the Raman scattering enhancement was evaluated by using methylene blue as target molecules. The Raman measurements demonstrated that the prepared substrates are reliable and sensitive with analytical enhancement factors, estimated to be around 105 with a concentration of methylene blue 1 μM. When triangular and hexagonal nanoplates were tested with different concentrations of analyte, they demonstrated a good linearity in Raman intensity with a good detection of methylene blue 0.1 μM.

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“…NIR optical nanoprobes have received increasing attention as multimodal therapeutic reagents for bioimaging and photothermal tumor elimination. For optical imaging, SERS has emerged as an attractive method with intense signal response and good specificity (Gandra and Singamaneni, 2013; Zhang et al, 2018). A narrow bandwidth, high resistance to photobleaching, and autofluorescence make SERS an effective tool for non-invasive single-cell assay, rapid disease diagnosis, and nanomedical imaging (Maiti et al, 2012; Guerrini et al, 2017).…”

Section: Optically Active Nanomedicine For Bioimagingmentioning

confidence: 99%

Optically Active Nanomaterials for Bioimaging and Targeted Therapy

Yang

Wang

Wan

et al. 2019

Front. Bioeng. Biotechnol.

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Non-invasive tracking for monitoring the selective delivery and transplantation of biotargeted agents in vivo has been employed as one of the most effective tools in the field of nanomedicine. Different nanoprobes have been developed and applied to bioimaging tissues and the treatment of diseases ranging from inflammatory and cardiovascular diseases to cancer. Herein, we will review the recent advances in the development of optics-responsive nanomaterials, including organic and inorganic nanoparticles, for multimodal bioimaging and targeted therapy. The main focus is placed on nanoprobe fabrication, mechanistic illustrations, and diagnostic, or therapeutical applications. These nanomedicine strategies have promoted a better understanding of the biological events underlying diverse disease etiologies, thereby facilitating diagnosis, illness evaluation, therapeutic effect, and drug discovery.

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SERS Nanotags and Their Applications in Biosensing and Bioimaging

Cited by 36 publications

References 151 publications

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

SERS Activity of Silver Nanosphere, Triangular Nanoplates, Hexagonal Nanoplates and Quasi-Spherical Nanoparticles: Effect of Shape and Morphology

Optically Active Nanomaterials for Bioimaging and Targeted Therapy

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