SERS-based sensor for the detection of sexually transmitted pathogens in the male swab specimens: A new approach for clinical diagnosis

Berus, Sylwia M.; Adamczyk-Popławska, Monika; Młynarczyk-Bonikowska, Beata; Witkowska, Evelin; Szymborski, Tomasz; Waluk, Jacek; Kamińska, Agnieszka

doi:10.1016/j.bios.2021.113358

Cited by 20 publications

(12 citation statements)

References 31 publications

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“…Recently, we determined the SERS profiles of gonococci—one of the most prevalent pathogens of the human urogenital tract [ 41 ]. This study aimed to record SERS signatures of bacteria (natural and causing BV), fungi, and protista— T. vaginalis —that may also be found in a human vagina and cause vaginal dysfunctions or STDs other than gonorrhea.…”

Section: Resultsmentioning

confidence: 99%

SERS-PLSR Analysis of Vaginal Microflora: Towards the Spectral Library of Microorganisms

Berus

Adamczyk-Popławska

Goździk

et al. 2022

IJMS

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The accurate identification of microorganisms belonging to vaginal microflora is crucial for establishing which microorganisms are responsible for microbial shifting from beneficial symbiotic to pathogenic bacteria and understanding pathogenesis leading to vaginosis and vaginal infections. In this study, we involved the surface-enhanced Raman spectroscopy (SERS) technique to compile the spectral signatures of the most significant microorganisms being part of the natural vaginal microbiota and some vaginal pathogens. Obtained data will supply our still developing spectral SERS database of microorganisms. The SERS results were assisted by Partial Least Squares Regression (PLSR), which visually discloses some dependencies between spectral images and hence their biochemical compositions of the outer structure. In our work, we focused on the most common and typical of the reproductive system microorganisms (Lactobacillus spp. and Bifidobacterium spp.) and vaginal pathogens: bacteria (e.g., Gardnerella vaginalis, Prevotella bivia, Atopobium vaginae), fungi (e.g., Candida albicans, Candida glabrata), and protozoa (Trichomonas vaginalis). The obtained results proved that each microorganism has its unique spectral fingerprint that differentiates it from the rest. Moreover, the discrimination was obtained at a high level of explained information by subsequent factors, e.g., in the inter-species distinction of Candida spp. the first three factors explain 98% of the variance in block Y with 95% of data within the X matrix, while in differentiation between Lactobacillus spp. and Bifidobacterium spp. (natural flora) and pathogen (e.g., Candida glabrata) the information is explained at the level of 45% of the Y matrix with 94% of original data. PLSR gave us insight into discriminating variables based on which the marker bands representing specific compounds in the outer structure of microorganisms were found: for Lactobacillus spp. 1400 cm−1, for fungi 905 and 1209 cm−1, and for protozoa 805, 890, 1062, 1185, 1300, 1555, and 1610 cm−1. Then, they can be used as significant marker bands in the analysis of clinical subjects, e.g., vaginal swabs.

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Section: Resultsmentioning

confidence: 99%

SERS-PLSR Analysis of Vaginal Microflora: Towards the Spectral Library of Microorganisms

Berus

Adamczyk-Popławska

Goździk

et al. 2022

IJMS

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“…Nevertheless, the silicon coated with noble metals as a substrate has demonstrated the best sensitivity/specificity/accuracy values (95.7%/95.1%/94.4%) and this illustrates the promising potential of the following substrate in clinical detection. The reason for such a claim is that there are already methods that demonstrated 100% accuracy [ 131 , 273 ], and with a slight optimization, the Au or Ag @ silicon-based substrates can be a good alternative to conventional substrates, while Ag@Al-based substrates already show comparable to gold and silver detection parameters.…”

Section: Sers Clinical Applicationsmentioning

confidence: 99%

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

et al. 2022

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This article compares the applications of traditional gold and silver-based SERS substrates and less conventional (Pd/Pt, Cu, Al, Si-based) SERS substrates, focusing on sensing, biosensing, and clinical analysis. In recent decades plethora of new biosensing and clinical SERS applications have fueled the search for more cost-effective, scalable, and stable substrates since traditional gold and silver-based substrates are quite expensive, prone to corrosion, contamination and non-specific binding, particularly by S-containing compounds. Following that, we briefly described our experimental experience with Si and Al-based SERS substrates and systematically analyzed the literature on SERS on substrate materials such as Pd/Pt, Cu, Al, and Si. We tabulated and discussed figures of merit such as enhancement factor (EF) and limit of detection (LOD) from analytical applications of these substrates. The results of the comparison showed that Pd/Pt substrates are not practical due to their high cost; Cu-based substrates are less stable and produce lower signal enhancement. Si and Al-based substrates showed promising results, particularly in combination with gold and silver nanostructures since they could produce comparable EFs and LODs as conventional substrates. In addition, their stability and relatively low cost make them viable alternatives for gold and silver-based substrates. Finally, this review highlighted and compared the clinical performance of non-traditional SERS substrates and traditional gold and silver SERS substrates. We discovered that if we take the average sensitivity, specificity, and accuracy of clinical SERS assays reported in the literature, those parameters, particularly accuracy (93–94%), are similar for SERS bioassays on AgNP@Al, Si-based, Au-based, and Ag-based substrates. We hope that this review will encourage research into SERS biosensing on aluminum, silicon, and some other substrates. These Al and Si based substrates may respond efficiently to the major challenges to the SERS practical application. For instance, they may be not only less expensive, e.g., Al foil, but also in some cases more selective and sometimes more reproducible, when compared to gold-only or silver-only based SERS substrates. Overall, it may result in a greater diversity of applicable SERS substrates, allowing for better optimization and selection of the SERS substrate for a specific sensing/biosensing or clinical application.

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“…In a very recent study, Berus et al 146 reported a rapid (<15 min), sensitive (10 2 CFU mL −1 ) and comprehensive SERS-chemometric analysis of NG in men's urethra swabs with another four bacterial pathogens, all being responsible for STDs, using silicon-based SERS substrates sputtered with 100 nm Ag layer. The method discussed the STD diagnosis in a direct and indirect manner, where the indirect (confirmatory) approach identified the unknown pathogenic strains in the clinical samples through matching their spectral images to other spectral images of different bacteria.…”

Section: Sers Applications For the “Big 5” Antibiotic Resistance Chal...mentioning

confidence: 99%

“…Therefore, this label and growth-free method demonstrated the potential of SERS to be a sensitive and real-time bioanalytical tool for studying the bacterial biochemical activity in cell wall and extracellular regions. Thus, providing a better treatment efficacy and disease prognosis than NAATs.In a very recent study, Berus et al146 reported a rapid (< 15 min), sensitive (10 2 CFU/mL) and comprehensive SERS-chemometric analysis of NG in men's urethra swabs with another four bacterial pathogens, all being responsible for STDs, using silicon-based SERS substrates sputtered with 100 nm Ag layer. The method discussed the STD diagnosis in a direct and indirect manner, where the indirect (confirmatory) approach identified the unknown pathogenic strains in the clinical samples through matching their spectral images to other spectral images of different bacteria.…”

mentioning

confidence: 99%

Recent advances in antibiotic resistance diagnosis using SERS: focus on the “Big 5” challenges

Hassanain

Johnson

Faulds

et al. 2022

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SERS-based sensor for the detection of sexually transmitted pathogens in the male swab specimens: A new approach for clinical diagnosis

Cited by 20 publications

References 31 publications

SERS-PLSR Analysis of Vaginal Microflora: Towards the Spectral Library of Microorganisms

SERS-PLSR Analysis of Vaginal Microflora: Towards the Spectral Library of Microorganisms

Trends in Application of SERS Substrates beyond Ag and Au, and Their Role in Bioanalysis

Recent advances in antibiotic resistance diagnosis using SERS: focus on the “Big 5” challenges

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