SERS-based rapid assay for sensitive detection of Group A<i>Streptococcus</i>by evaluation of the swab sampling technique

Eryılmaz, Merve; Soykut, Esra Acar; Çetin, Demet; Boyaci, İsmail Hakkı; Suludere, Zekiye; Tamer, Uğur

doi:10.1039/c9an00173e

Cited by 24 publications

(12 citation statements)

References 39 publications

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“…As aforementioned, LFAs are used in a variety of applications with samples that present extremely different composition and characteristics (Table 1). 27 For example, clinical samples can be whole blood, 28,29 plasma, 30,31 serum, [32][33][34] sweat, 35,36 urine, 37,38 stool, 39,40 saliva, 41,42 cerebrospinal fluid 43,44 and nasal swabs, 45,46 while food matrices could be juices, 47,48 cereals, 49,50 meat, 51,52 vegetables, 53 and environmental (mostly water and soil) samples. [54][55][56][57] Although the sample pad provides a means to control the properties of the sample solution (see the following section), some complex matrixes may require pre-treatment before an aliquot can be added into the LFA strip.…”

Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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Lateral flow assays (LFA) are quick, simple and cheap assays to analyse a variety of samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample within a series of sequential pads with different functionalities aiming to generate a signal indicating the absence/presence (and, in some cases, the concentration) of the analyte of interest. In order to have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this Tutorial we provide the readers with: 1) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, 2) a roadmap for optimal LFA development independent of the specific application, 3) a step by step example protocol for the assembly and operation of an LF strip for the detection of Human Immunoglobulin G and 4) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs.

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Section: Types Of Samples and Target Analytesmentioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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“…The latest innovations aimed at improving the analytical characteristics of the LFIA technology are interesting, promising, and can provide these platforms with additional advantages (e.g., integration into the ‘chip-based laboratory’ design) [66] . Nevertheless, most of them increase cost of test systems, their complexity, and, thus, reduce availability of these remarkable technologies, a property that has made them popular and attractive for the recent six decades.…”

Section: Discussionmentioning

confidence: 99%

Six decades of lateral flow immunoassay: from determining metabolic markers to diagnosing COVID-19

2020

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Technologies based on lateral flow immunoassay (LFIA), known in some countries of the world as immunochromatographic tests, have been successfully used for the last six decades in diagnostics of many diseases and conditions as they allow rapid detection of molecular ligands in biosubstrates. The popularity of these diagnostic platforms is constantly increasing in healthcare facilities, particularly those facing limited budgets and time, as well as in household use for individual health monitoring. The advantages of these low-cost devices over modern laboratory-based analyzers come from their availability, opportunity of rapid detection, and ease of use. The attractiveness of these portable diagnostic tools is associated primarily with their high analytical sensitivity and specificity, as well as with the easy visual readout of results. These qualities explain the growing popularity of LFIA in developing countries, when applied at small hospitals, in emergency situations where screening and monitoring health condition is crucially important, and as well as for self-testing of patients. These tools have passed the test of time, and now LFIA test systems are fully consistent with the world's modern concept of ‘point-of-care testing’, finding a wide range of applications not only in human medicine, but also in ecology, veterinary medicine, and agriculture. The extensive opportunities provided by LFIA contribute to the continuous development and improvement of this technology and to the creation of new-generation formats. This review will highlight the modern principles of design of the most widely used formats of test-systems for clinical laboratory diagnostics, summarize the main advantages and disadvantages of the method, as well as the current achievements and prospects of the LFIA technology. The latest innovations are aimed at improving the analytical performance of LFIA platforms for the diagnosis of bacterial and viral infections, including COVID-19.

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“…For example, they have been used as signal generators to improve sensitivity in diverse readout strategies (using chromophores and fluorescent dyes) such as surface-enhanced Raman spectroscopy (SERS) over the usual or standard LFA. [79,[83][84][85] Intriguingly, other different nanomaterials can be combined to enhance sensitivity, as has been shown through use of graphene to quench quantum dots as a revealing agent in LFAs for detection of bacteria, or the use of magnetic nanoparticles for preconcentration and purification of the sample. [86][87][88][89][90]…”

Section: Nanomaterials and Lfasmentioning

confidence: 99%

The Microbiome Meets Nanotechnology: Opportunities and Challenges in Developing New Diagnostic Devices

et al. 2021

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Monitoring of the human microbiome is an emerging area of diagnostics for personalized medicine. Here, the potential of different nanomaterials and nanobiosensing technologies is reviewed for the development of novel diagnostic devices for the detection and measurement of microbiome‐related biomarkers. Moreover, the current and future landscape of microbiome‐based diagnostics is defined by exploring the advantages and disadvantages of current nanotechnology‐based approaches, especially in the context of developing point‐of‐care (PoC) devices that would meet the international guidelines known as REASSURED (Real‐time connectivity; Ease of specimen collection; Affordability; Sensitivity; Specificity; User‐friendliness; Rapid & robust operation; Equipment‐free; and Deliverability). Finally, the strategies of the latest international scientific consortia working in this field are analyzed, the current microbiome diagnostics market are reported and the principal ethical, legal, and societal issues related to microbiome R&D and innovation are discussed.

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SERS-based rapid assay for sensitive detection of Group AStreptococcusby evaluation of the swab sampling technique

Abstract: This study combines the accuracy of the gold standard with the sensitivity of SERS for fast detection of Group A Streptococcus pyogenes.

Cited by 24 publications

References 39 publications

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Six decades of lateral flow immunoassay: from determining metabolic markers to diagnosing COVID-19

The Microbiome Meets Nanotechnology: Opportunities and Challenges in Developing New Diagnostic Devices

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