SERS-based immunoassay using a gold array-embedded gradient microfluidic chip

Lee, Moonkwon; Lee, Kangsun; Kim, Ki Hyung; Oh, Kwang W.; Choo, Jaebum

doi:10.1039/c2lc40353f

Cited by 113 publications

(102 citation statements)

References 43 publications

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“…The decoration of antibodies increases the sensitivity and specificity of SERS sensors to corresponding antigens or bacteria [62][63][64][65][66]. Labeled DNA oligonucleotides can be identified by complementary nucleotides decorated within SERS microchannels.…”

Section: Detection Of Biomassmentioning

confidence: 99%

Review of microfluidic approaches for surface-enhanced Raman scattering

Zhou

Kim

2016

Sensors and Actuators B: Chemical

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Section: Detection Of Biomassmentioning

confidence: 99%

Review of microfluidic approaches for surface-enhanced Raman scattering

Zhou

Kim

2016

Sensors and Actuators B: Chemical

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“…34 In addition, Choo's group relied on this theory and realized the SERS-based immunoassay and DNA detection. 35,36 As shown in Fig. 1, this micromixer could be applied as an efficient microfluidic channel for SERS detection of the chemical and biological analytes.…”

Section: Micromixermentioning

confidence: 99%

“…143,144 By using Kwang's novel gradient method, Lee developed a programmable and fully automatic gold array-embedded gradient optofluidic chip for Raman ELISA analysis. 35 The microfluidic channel matches the size of bacteria very well which is conducive to the cultivation of the bacteria or cell 141,[145][146][147] that offers the possibility of system integration issues for bio/chemical molecular analysis at the trace and even single molecule level. 3,148,149 Walter et al 150 presented a fast, reliable and specific test of bacteria by means of SERS in a microfluidic device (Fig.…”

Section: Biological and Biomedicalmentioning

confidence: 99%

Surface-enhanced Raman scattering microfluidic sensor

Wang

2013

RSC Adv.

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Surface-enhanced Raman scattering (SERS) is a highly sensitive detection technique used to provide information about chemical and biological trace analytes. The confocal Raman microscope makes it possible to monitor small samples in a narrow microfluidic channel, despite the fact that the scattering intensity of the Raman signal is very weak. Recently, microfluidic chip devices coupled with on-chip SERS detection method and their applications to sensitive chemical and biological analyses have attracted significant attention. In this mini-review, highlights of advances in SERS techniques, microfluidic devices and their applications on the biological/environmental analysis of minute analytes within the recent years are provided to illustrate the impact of this rapidly growing area of research.

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“…[10][11][12] The most popular platform for the SERS-based immunoassay is the detection of a sandwich immunocomplexes on a planar gold substrate. 13,14 Here, the capture antibody is bound to a planar substrate, while the detection antibody-conjugated SERS nano tag is coupled to an antigen in the sandwiched format. In this case, however, due to slower kinetics of protein-protein interactions derived from binding restriction near the surface, the assay time consumed is relatively long.…”

Section: 9mentioning

confidence: 99%

Ultrasensitive Detection of Angiogenin Using Surface-Enhanced Raman Scattering Immunoassay Platform

Chon¹,

Chang²,

Lim³

et al. 2013

Bulletin of the Korean Chemical Society

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Angiogenesis, the process of new blood-vessel growth, plays an important role in normal physiological process. 1,2To date, angiogenin (ANG) is known to be a key factor in induction of angiogenesis by activation of endothelial and smooth muscle cells as well as by triggering a number of biological processes.3-5 It is also well known that the expression of ANG is up-regulated in various types of human cancers. 4,5 This indicates that there is an intimate relationship between the expression of ANG and tumor development. Therefore, it is important to develop a highly sensitive immunoassay technique for ANG. At present, enzyme-linked immunosorbent assay (ELISA) is the most widely used immunoassay method in detection of the presence of ANG. 6,7Here, the fluorescence detection using molecular labels is generally used in most of cases. However, the poor limit of detection, long assay time, and photo-bleaching effect often limit its potential applications. 8,9Recently, surface-enhanced Raman scattering (SERS) detection became a promising alternative to resolve the problems of fluorescence detection.10-12 The most popular platform for the SERS-based immunoassay is the detection of a sandwich immunocomplexes on a planar gold substrate.13,14 Here, the capture antibody is bound to a planar substrate, while the detection antibody-conjugated SERS nano tag is coupled to an antigen in the sandwiched format. In this case, however, due to slower kinetics of protein-protein interactions derived from binding restriction near the surface, the assay time consumed is relatively long. In addition, multiple washing steps to remove nonspecific binding proteins make the SERS-based assay on the two-dimensional gold substrate less convenient.To resolve these problems, we recently developed a new SERS-based assay protocol using SERS nano tags and magnetic beads.15-17 In this work, 1 µm magnetic beads (Dynabeads ® MyOne TM Carboxylic Acid, Invitrogen) were used as supporting substrate instead of two-dimensional gold substrate. This magnetic bead-based immunoassay overcomes the slow immunoreaction derived from the diffusion-limited kinetics on gold planar substrates caused by the high surface-tovolume ratio of the magnetic beads. Consequently, assay time is greatly reduced to less than 30 min including immunocomplex formation, washings and detection. In the present work, we explore the applicability of the SERS-based detection using hollow gold nanospheres (HGNs) SERS nano tags and magnetic beads for fast and ultrasensitive immunoassay of ANG. HGNs show reproducible and sensitive localized surface plasmon effects from individual particles because hot junctions can be localized on the pinholes of the particle surface. For the validation of this detection technique, the limit of detection (LOD) for the ANG marker will be compared with the result using ELISA. Figure 1 displays a schematic layout of the SERS-based sandwich immunoassay for ANG. HGNs and magnetic beads were used as SERS nano tags and supporting substrates, respectively. Antibody conj...

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SERS-based immunoassay using a gold array-embedded gradient microfluidic chip

Cited by 113 publications

References 43 publications

Review of microfluidic approaches for surface-enhanced Raman scattering

Review of microfluidic approaches for surface-enhanced Raman scattering

Surface-enhanced Raman scattering microfluidic sensor

Ultrasensitive Detection of Angiogenin Using Surface-Enhanced Raman Scattering Immunoassay Platform

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