SERS based immuno-microwell arrays for multiplexed detection of foodborne pathogenic bacteria

Sun, Jian; Hankus, Mikella E.; Cullum, Brian M.

doi:10.1117/12.817608

Cited by 8 publications

(2 citation statements)

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“…The labeled approach is by far a more active research area in SERS bacteria detection. Singleplex and multiplex detection of E. coli, S. aureus, Campylobacter, Listeria, and Bacillus anthracis spores coupled with immunomagnetic separation (IMS), microfluidics, and filtration have been reported (19,43,77,104,121,134). Detection has been evaluated in some food matrices, including ground beef, peanut butter, apple juice, and orange juice (19,43,77,121).…”

Section: Detection Methods Based On Nanomaterialsmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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Bacterial pathogens are one of the leading causes of food safety incidents and product recalls worldwide. Timely detection and identification of microbial contamination in agricultural and food products is crucial for disease prevention and outbreak investigation. In efforts to improve and/or replace time-consuming and laborious "gold standards" for pathogen detection, numerous alternative rapid methods have been proposed in the past 15 years, with a trend toward incorporating nanotechnology and nanomaterials in food pathogen detection. This article is a review of the use of nanotechnology in various detection and sample preparation techniques and advancements in nanotechnology applications in food matrices. Some practical considerations in nanobioassay design are discussed, and the gaps between research status quo and market demands are identified.

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Section: Detection Methods Based On Nanomaterialsmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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“…[1][2][3] Of these sensors, those based on surface-enhanced Raman spectroscopy (SERS) offer several advantages over those employing other spectroscopic techniques, including being capable of multiplexed detection of target species (due to the narrow spectral linewidth), molecular recognition (associated with vibrational spectroscopy), and extremely sensitive analysis. [4][5][6] Despite these advantages, SERS sensors are not as commonly employed for real-world applications as other techniques (e.g., fluorescence) often due to issues with sensitivity and reproducibility. 1,7 One well-cited downfall of SERS sensing is the inherently weak Raman signals that must be enhanced.…”

Section: Introductionmentioning

confidence: 99%

Improving Sensitivity and Reproducibility of SERS Sensing in Microenvironments Using Individual, Optically Trapped Surface-Enhanced Raman Spectroscopy(SERS) Probes

2016

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Surface-enhanced Raman spectroscopy (SERS) sensors offer many advantages for chemical analyses, including the ability to provide chemical specific information and multiplexed detection capability at specific locations. However, to have operative SERS sensors for probing microenvironments, probes with high signal enhancement and reproducibility are necessary. To this end, dynamic enhancement of SERS (i.e., in-situ amplification of signal-to-noise and signal-to-background ratios) from individual probes has been explored. In this paper, we characterize the use of optical tweezers to amplify SERS signals as well as suppress background signals via trapping of individual SERS active probes. This amplification is achieved through a steady presence of a single "hot" particle in the focus of the excitation laser. In addition to increases in signal and concomitant decreases in non-SERS backgrounds, optical trapping results in an eightfold increase in the stability of the signal as well. This enhancement strategy was demonstrated using both single and multilayered SERS sub-micron probes, producing combined signal enhancements of 24-fold (beyond the native 10 SERS enhancement) for a three-layered geometry. The ability to dynamically control the enhancement offers the possibility to develop SERS-based sensors and probes with tailored sensitivities. In addition, since this trapping enhancement can be used to observe individual probes with low laser fluences, it could offer particular interest in probing the composition of microenvironments not amenable to tip-enhanced Raman spectroscopy or other scanning probe methods (e.g., intracellular analyses, etc.).

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Surface enhanced Raman scattering (SERS)-based next generation commercially available substrate: physical characterization and biological application

2011

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SERS based immuno-microwell arrays for multiplexed detection of foodborne pathogenic bacteria

Cited by 8 publications

References 0 publications

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Improving Sensitivity and Reproducibility of SERS Sensing in Microenvironments Using Individual, Optically Trapped Surface-Enhanced Raman Spectroscopy(SERS) Probes

Surface enhanced Raman scattering (SERS)-based next generation commercially available substrate: physical characterization and biological application

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