SERS as tool for the analysis of DNA-chips in a microfluidic platform

Strelau, Katharina K.; Kretschmer, Robert; Möller, Robert; Fritzsche, Wolfgang; Popp, J.

doi:10.1007/s00216-009-3374-8

Cited by 58 publications

(44 citation statements)

References 15 publications

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“…3 is generally in agreement with those reported earlier [21] showing characteristic Raman lines at 739 cm À1 and 1339 cm À1 corresponding to the purine ring breathing mode and CN stretching mode, respectively. The ultra-sensitive detection of adenine suggests that monitoring of DNA sequencing on the single-molecule level is possible on this engineered substrate after further optimization to attain higher detection limitation or detecting the mismatch of DNA sequences after the integration to a chip-based platform [22,23]. For further confirmation, adenosine is chosen as another target molecule because of the important role played by adenosine triphosphate (ATP) molecules in biological energyetransfer reactions.…”

Section: Resultsmentioning

confidence: 99%

Atomic layer deposition of platinum thin films on anodic aluminium oxide templates as surface-enhanced Raman scattering substrates

Zhang

Qiu

et al. 2013

Vacuum

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Section: Resultsmentioning

confidence: 99%

Atomic layer deposition of platinum thin films on anodic aluminium oxide templates as surface-enhanced Raman scattering substrates

Zhang

Qiu

et al. 2013

Vacuum

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“…[13] Compared to chip-based methods [14][15][16][17][18][19][20][21][22] the use of magnetic particles benefits from improved hybridization kinetics. By employing biotin-labeled primers for the amplification of target DNA with polymerase chain reaction (PCR), the PCR products can bind directly onto the magnetic beads.…”

Section: Magnetic Particlesmentioning

confidence: 99%

Discrimination of clostridium species using a magnetic bead based hybridization assay

Pahlow

Seise

Pollok

et al. 2014

Biophotonics: Photonic Solutions for Better Health Care IV

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Clostridium chauvoei is the causative agent of blackleg, which is an endogenous bacterial infection. Mainly cattle and other ruminants are affected. The symptoms of blackleg are very similar to those of malignant edema, an infection caused by Clostridium septicum. [1, 2] Therefore a reliable differentiation of Clostridium chauvoei from other Clostridium species is required. Traditional microbiological detection methods are time consuming and laborious. Additionally, the unique identification is hindered by the overgrowing tendency of swarming Clostridium septicum colonies when both species are present. [1, 3, 4] Thus, there is a crucial need to improve and simplify the specific detection of Clostridium chauvoei and Clostridium septicum.Here we present an easy and fast Clostridium species discrimination method combining magnetic beads and fluorescence spectroscopy. Functionalized magnetic particles exhibit plentiful advantages, like their simple manipulation in combination with a large binding capacity of biomolecules. A specific region of the pathogenic DNA is amplified and labelled with biotin by polymerase chain reaction (PCR). These PCR products were then immobilized on magnetic beads exploiting the strong biotin-streptavidin interaction. The specific detection of different Clostridium species is achieved by using fluorescence dye labeled probe DNA for the hybridization with the immobilized PCR products. Finally, the samples were investigated by fluorescence spectroscopy. [5]

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“…To solve this situation, single-and doublestranded thiolated DNA or RNA oligomers have been used, which can direct the adsorption of the macromolecule to be perpendicular to the surface (Barhoumi et al 2008). Further, other recent approaches include the combination of SERS with microfluidic systems for sequence-specific detection of DNA (Strelau et al 2010).…”

Section: Direct Sers Sensingmentioning

confidence: 99%

Surface-enhanced Raman scattering biomedical applications of plasmonic colloidal particles

Abalde‐Cela

Aldeanueva-Potel

Mateo-Mateo

et al. 2010

J. R. Soc. Interface.

198

177

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This review article presents a general view of the recent progress in the fast developing area of surface-enhanced Raman scattering spectroscopy as an analytical tool for the detection and identification of molecular species in very small concentrations, with a particular focus on potential applications in the biomedical area. We start with a brief overview of the relevant concepts related to the choice of plasmonic nanostructures for the design of suitable substrates, their implementation into more complex materials that allow generalization of the method and detection of a wide variety of (bio)molecules and the strategies that can be used for both direct and indirect sensing. In relation to indirect sensing, we devote the final section to a description of SERS-encoded particles, which have found wide application in biomedicine (among other fields), since they are expected to face challenges such as multiplexing and high-throughput screening.

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SERS as tool for the analysis of DNA-chips in a microfluidic platform

Cited by 58 publications

References 15 publications

Atomic layer deposition of platinum thin films on anodic aluminium oxide templates as surface-enhanced Raman scattering substrates

Atomic layer deposition of platinum thin films on anodic aluminium oxide templates as surface-enhanced Raman scattering substrates

Discrimination of clostridium species using a magnetic bead based hybridization assay

Surface-enhanced Raman scattering biomedical applications of plasmonic colloidal particles

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