SERRS immunoassay for quantitative human CRP analysis

Campbell, Fiona; Ingram, Andrew; Monaghan, Paul; Cooper, Jonathan M.; Sattar, Naveed; Eckersall, P. D.; Graham, Duncan

doi:10.1039/b808087a

Cited by 35 publications

(29 citation statements)

References 25 publications

(18 reference statements)

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“…Another benefit of using NPs in ELISAs is the ability to utilise their roughened metal surface by adsorbing colorimetric probes onto them and analysing using surface enhanced resonance Raman scattering (SERRS) which was first reported by Campbell et al 9 When the ELISA substrate bromochloroindolylphosphate (BCIP), was added to the sandwich ELISA with an antibody conjugated to alkaline phosphate, a blue dimeric species was produced which could be adsorbed onto Au NPs and a strong SERRS signal was achieved and correlated to the concentration of CRP. Graham et.…”

Section: Introductionmentioning

confidence: 99%

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Sloan-Dennison

Laing

Shand

et al. 2017

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aArtificial enzymes have become an increasingly interesting area of research due to their many advantages over natural protein enzymes which are expensive, difficult to isolate and unable to stand harsh environments. An important area of this research involves using metal nanoparticles as artificial enzymes, known as nanozymes, which exhibit peroxidase-like activity enabling them to catalyse the oxidation of substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), giving a colorimetric response. Here we exploit the catalytic activity of silver nanoparticles (Ag NPs) in a surface based silver-linked immunosorbent assay (SLISA) to detect human C-reactive protein (CRP), an inflammatory marker. Ag NPs were conjugated to antibodies with specific recognition for the corresponding target antigenic molecule, CRP, and the Ag NPs were used to catalyse the oxidation of TMB by H2O2. The resulting coloured oxidation product was detected using SERRS. We demonstrate that Ag NPs can replace the enzymes used in a conventional ELISA and a detection limit of 1.09 ng/mL of CRP can be achieved. It indicates the promise for SLISAs for biomarker detection and opens the way for further assays of this nature to be created. This novel assay has the potential to be optimised to detect lower levels of CRP and can be further extended for the sensitive and specific detection of other relevant biomarkers.

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Section: Introductionmentioning

confidence: 99%

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Sloan-Dennison

Laing

Shand

et al. 2017

Analyst

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“…Due to its sensitivity and ability to readily discriminate multiple components simultaneously, SERRS spectroscopy is a highly desirable technique for a wide range of analytical and bioanalytical applications [6][7][8] . SERRS detection of functionalized nanosensors is increasingly being used in assays of significant biomedical interest, such as cancer tumour detection 9 , immunoassays 10 and DNA detection 11,12 . SERRS detection is also increasingly being employed in tandem with microdroplet and microemulsions for fast, low volume, high-throughput analyses 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of individual microdroplets by SERRS spectroscopy

et al. 2012

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Raman spectroscopy and its various derivatives continue to offer the analyst fast, powerful, non-invasive and nondestructive means by which to identify multiple analytes simultaneously and in real time. By virtue of the huge enhancements possible in Raman scattering, generated by both surface enhancement and the resonance Raman effect, or when coupled with other techniques such as confocal microscopy, Raman spectroscopy is becoming more and more applicable to the types of assay being conducted in lab-on-a-chip applications, such as flow cytometry, cell patterning and trapping, and microarrays, all of which often involve the detection of extremely low quantities of analyte. Surface enhanced Raman scattering (SERS, or when coupled with the resonance Raman phenomenon, SERRS) spectroscopy has proven to be of particular use as a robust optical detection method in microfluidic environments. In this paper, we demonstrate the use of SERRS multiplex detection to quantitatively characterize individual microdroplets in a continuous stream whose contents are gradually varied using a bespoke pump control algorithm.

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“…There is much freedom over the type of antibody label and selection is dependent on the specific detection methodology. This includes fluorescent labels, [18][19][20][21][22][23][24][25][28][29][30][31][32][33]35,[38][39][40]72,73 enzymes (for catalysis of a colour changing/redox reactions), [43][44][45][46][47]61,[80][81][82]97,98,[100][101][102] paramagnetic particles (inductance/magnetic field based measurement) [112][113][114]116,117 and metallic colloids as SERS probes. 78,79,83,84 Various research groups have also investigated the possibility of label free immunoassay biosensors, 58,59,61,[94][95]…”

Section: Introduction To Immunoassaysmentioning

confidence: 99%

Lab-on-a-chip based immunosensor principles and technologies for the detection of cardiac biomarkers: a review

2011

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This review examines the current state of the art lab-on-a-chip and microfluidic based biosensor technologies used in the detection of cardiac biomarkers. The determination and quantification of blood based, cardiac biomarkers are crucial in the triage and management of a range of cardiac related conditions, where time delay has a major impact on short and longer-term outcomes of a patient. The design and manufacturing of biomarker detection systems are multi-disciplinary in nature and require researchers to have knowledge of both life sciences and engineering for the full potential of this field to be realised. This review will therefore provide a comprehensive overview of chip based immunosensing technology as applied to cardiac biomarker detection, while discussing the potential suitability and limitations of each configuration for incorporation within a clinical diagnostics device suitable for point-of-care applications.

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SERRS immunoassay for quantitative human CRP analysis

Cited by 35 publications

References 25 publications

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Characterization of individual microdroplets by SERRS spectroscopy

Lab-on-a-chip based immunosensor principles and technologies for the detection of cardiac biomarkers: a review

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