Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies

Abstract

A panel of 15 monoclonal antibodies was prepared that could distinguish among the 15 serovars of Chiamydia trachomatis. Twelve of these antibodies were specific for a single serovar (A, B, C, D, E, F, G, H, I, K, L1, and L2) and three were specific for two serovars (B/Ba, C/J, and C/L3). Ten of the serovar-specific and two of the bispecific antibodies were shown by immunoblotting to recognize epitopes on the major outer membrane protein. These data provide evidence that such epitopes are closely correlated with and may be partly responsible for the antigenic variations detected by microimmunofluorescence that distinguish the currently recognized serovars. When used in a radioimmunoassay, these antibodies correctly identified the serovar of 17 strains that had been serotyped by the microimmunofluorescence test. In addition, we found that the chlamydial antigen derived from 1.0 cm2 of an infected HeLa cell monolayer was sufficient to allow serotyping with these antibodies. Thus, these monoclonal antibodies may provide a rapid and reliable alternative to mouse immunization and microimmunofluorescence for serotyping of clinical isolates.

KN-62 enhances Chlamydia pneumoniae-induced p44/p42 mitogen-activated protein kinase activation in murine fibroblasts and attenuates in vitro infection

“…The microorganism was raised for 48^72 h in cycloheximide-treated HEp-2 cells. Elementary bodies (EBs) were harvested and puri¢ed by soni¢cation, di¡erential centrifugation and Percoll density gradient centrifugation as described elsewhere [14]. C. pneumoniae EBs were stored at 380 ‡C and the titer of the stock was determined as inclusion-forming units (IFU) in immuno£uorescence (IFA) using a genus-speci¢c monoclonal antibody to chlamydial lipopolysaccharide (LPS) antigen [15].…”

Identification of a Chlamydia trachomatis Serovar E Urogenital Isolate Which Lacks the Cryptic Plasmid

“…1). The nucleotide sequence of the C599 omp1 was identical to that of E/Bour (10), and the E serotype was confirmed by monoclonal antibody staining (8). The 68 pCT PCR-negative, culture-negative patient samples from the study population also were omp1 PCR negative, indicating that plasmidless strains are a relatively rare phenomenon.…”

“…Both urine and urethral swab specimens were obtained. Chlamydia organisms from the urethral swab specimen were expanded in McCoy cell monolayers (5), and inclusions were stained with a genus-specific antilipopolysaccharide antibody (8). Amplification of DNA from urine was done as described by the manufacturer (PCR was done with the Amplicor kit from Roche, and LCR was done with the LCx kit from Abbott Labs).…”

Differences in IgG 1 and IgG 2 Responses of Goats to Chlamydial Abortions and to Clinically Inapparent Infections Detected by the Western Blot Technique

“…MCCAULY and TIEKEN (10) Antigenic analysis of C. trachomatis has revealed genus-, species-, subspecies-, and type-specific antigenic determinants (9). Applying type-specific murine antisera or monoclonal antibodies, C. trachomatis can currently be categorized into 15 serotypes (14,28,29). Depending on the serotype involved, C. truchomutis can cause diseases in man such as lymphogranuloma venereum, trachoma, inclusion conjunctivitis, pneumonitis and, with increasing significance, genital tract diseases.…”