Serotyping, RAPD Grouping and Antibiotic Susceptibility Testing of Salmonella Enterica Isolated from Retail Foods in Thailand

Abstract

and 7%, respectively, tested positive for Salmonella spp., whereas the corresponding contamination rates were 5%, 5%, 0% and 0% for the supermarket samples. The resistance of the isolates towards sixteen antibiotics was tested.A high percentage of the isolates were resistant to streptomycin, tetracycline or ampicillin: 78%, 59% and 51%, respectively. In total, 152 isolates were resistant to at least one antibiotic, and of these, 98 (64%) were resistant to at least three antibiotics (multidrug-resistant). The most frequently isolated serovars were Corvallis, Rissen and O4: i:-. Random Amplified Polymorphic DNA (RAPD) was used for grouping 149 isolates into five groups. The grouping of the isolates is baseline information to emphasize the usefulness of subtyping Salmonella spp. isolated from several categories of retail foods obtained from free markets and supermarkets by using antibiotic resistance studies and RAPD grouping.

Comparative meta-analysis of antimicrobial resistance from different food sources along with one health approach in Italy and Thailand

“…Out of 112 research papers related to foodborne pathogens in Thailand from 2012 to 2021, 33 were considered for meta-analysis [ 4 , 31 , 34 , 35 , [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] , [77] , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] , [88] , [89] , [90] , [91] , [92] , [93] , [94] , [95] , [96] , [97] ]. Among 11,753 AMR isolates 3526 (30%) were represented by E. coli 1744 (14.84%), Salmonella spp.…”

“…In Thailand, fresh foods sold at open markets such as meat, eggs and sea-food represent an important source of foodborne diseases due to their easy contamination by pathogens [ [30] , [31] , [32] , [33] , [34] ]. The food conservation at environmental temperature, without strict hygienic and temperature control, favors cross-contamination in storing facilities and distributing channels like shipping from slaughterhouse to the markets [ 35 , 36 ]. Consequently, Taiwan and India has adapted several plans to limit AMR and MDR spreading.…”

Application of actinomycetes in the control of Salmonella species

“…Salmonella is a gram-negative, motile, non-spore-forming, rod-shaped facultative anaerobic bacteria that belong to the Enterobacteriaceae family. 15,29 Salmonella species enterica and bongori are further classified into six subspecies based on the taxonomy created by Le Minor and Popoff in 1987. 27,15 Because it was formerly believed that each serotype had its own species, the genus Salmonella was previously divided into a number of other species.…”