Serotyping of Chlamydia: isolates of bovine origin

Abstract

Chlamydial isolates of bovine origin were serotyped by a plaque reduction method. Of the two major serotypes observed, type 1 included isolates from bovine abortion and enteric infections, whereas type 2 isolates were associated with polyarthritis or encephalomyelitis. These two serotypes were identical to those with a similar disease distribution previously observed in isolates of ovine origin. The two groups did not cross-react and they were serologically unrelated to chlamydiae of avian origin. Thus, it appears that many chlamydial isolates causing intestinal infections or abortion in sheep or cattle are closely related antigenically, as are those producing polyarthritis, encephalomyelitis, and conjunctivitis, and that the two groups are distinct.

A MODIFIED ISOLATION TECHNIQUE FOR CHLAMYDIA PSITTACI IN L‐CELLS TREATED WITH CYCLOHEXIMIDE AND GLUCOCORTICOID

“…In order to predict time post infection at which optimal numbers of inclusions for any possible variant might be expected, a time study was undertaken. which also included a less cytopathogenic strain (29) of the same serotype (25). The efficiency of this system was finally compared with isolation in embryonated eggs, using different isolates from a clinical case.…”

“…For C. trachomaris this work has promoted the understanding of chlamydia as an important etiological agent of especially venereal diseases of man in industrialized countries (24). For C. psirfaci, only including two serotypes (25). which are mainly restricted to infections in mammals and birds (281, experimental work with some strains, propagating readily in cell cultures without any artificial aid, has been reported (17, 16, 7, 2.…”

Chlamydia pecorum the Fourth Species of Genus Chlamydia

“…Storz and his coworkers (28,32,33) showed 8 biotypes in avian and mammalian C. psittaci by analysis of morphological differences of inclusion bodies and biological effects of diethylaminoethyl (DEAE)-dextran and cycloheximide in their growth medium, and further revealed 9 immunotypes in mammalian C. psittaci by applying microimmunofluorescent test (MIF). Eb and Orfila (4) also confirmed the 2 serotypes of Schachter et al (30,31). Recently Anderson (1) reported serotyping by use of typespecific monoclonal antibodies to avian and mammalian C. psittaci.…”

“…Many efforts were attempted to classify C. psittaci. Schachter et al (30,31) showed 2 serotypes in mammalian C. psittaci using plaque neutralization tests. Storz and his coworkers (28,32,33) showed 8 biotypes in avian and mammalian C. psittaci by analysis of morphological differences of inclusion bodies and biological effects of diethylaminoethyl (DEAE)-dextran and cycloheximide in their growth medium, and further revealed 9 immunotypes in mammalian C. psittaci by applying microimmunofluorescent test (MIF).…”

Immunotyping of Chlamydia psittaci by Indirect Immunofluorescence Antibody Test with Monoclonal Antibodies

“…The association of human psittacosis cases with psittacine birds and pigeons is well recognized (19,20,22). Serotyping or biotyping of C. psittaci has been reported by several investigators (4,18,20,24,25) ; however, their work dealt mostly with C. psittaci strains of mammalian origin, leaving serotyping of avian strains unexplored. In contrast, Chlamydia trachomatis strains have been divided into 15 serotypes by the micro-immunofluorescence test (MIFT) (14,27).…”