Chlamydia pecorum the Fourth Species of Genus Chlamydia

福士, 秀人; 平井, 克哉

doi:10.12935/jvma1951.48.1

Cited by 19 publications

(25 citation statements)

References 14 publications

(21 reference statements)

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“…l). The overall G+C content of the cloned DNA was calculated to 40.1 mo1/100 mol which was in good agreement with 39.6 mol/l00 mol published for C. psittaci (Fukushi and Hirai, 1993). …”

Section: Resultssupporting

confidence: 84%

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Molecular Cloning, Sequence Analysis and Functional Characterization of the Gene Kdsa, Encoding 3‐Deoxy‐D‐Manno‐2‐Octulosonate‐8‐Phosphate Synthase of Chlamydia Psittaci 6BC

Brabetz¹,

Brade²

1997

European Journal of Biochemistry

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The kdsA gene encoding 3-deoxy-~-manno-2-octulosonate-8-phosphate (Kdo-8-P) synthase of Chlamydia psittaci 6BC was cloned by complementing the temperature-sensitive kdsA mutant Salmonella enterica serovar Typhimurium AG701i50. The sequence analysis of a recombinant DNA fragment revealed an open reading frame of 807 nucleotides which codes for a polypeptide of 269 amino acids with a high degree of similarity to known KdsA proteins. In addition, alignments of Kdo-8-P synthases with bacterial and fungal 3-deoxy-~-arabino-2-heptulosonate-7-phosphate (Dha-7-P) synthases suggested that both classes of enzymes are structurally related and may belong to a family of 2-keto-3-deoxy-aldonic acid synthases. The chlamydial protein was overexpressed and functionally characterized in vitro to synthesize Kdo-8-P from D-arabinose 5-phosphate and phosphoennlpyruvate. A chlamydial DNA region upstream of the gene exhibiting similarities to the consensus sequence of a ' " promoters of Escherichia coli was responsible for the heterologous expression of kdsA.Keywords: 3-deoxy-~-manno-2-octulosonate-8-phosphate synthase; lipopolysaccharide biosynthesis; Chlamydia.Lipopolysaccharides (LPS) are amphiphilic molecules anchored in the outer leaflet of the outer membrane of gram-negative bacteria (Rietschel et al., 1994). In general, the architecture of LPS consists of a phosphorylated and acylated p(l+6)-linked glucosainine disaccharide, termed lipid A, to which a variable carbohydrate moiety is covalently linked. For genetic and biosynthetic reasons the sugar moiety may be further divided into a lipid-A proximal core and an outer 0-antigen region (Schnaitman and Klena, 1993). As surface structures, LPS play an important role in the interaction of gram-negative bacteria with higher organisms and are responsible for many immunological and pathophysiological effects which are observed during infectious diseases. On the other hand, many cellular characteristics of gram-negative bacteria like motility or the resistance to antibiotics may be correlated with biophysical and biochemical properities of LPS (Schnaitman and Klena, 1993;Nikaido, 1994). In particular, it is known from the structural analysis of various species, as well as mutants with defects in the biosynthesis of the inner core, that 3-deoxy-D-manno-2-octu~osonic acid (Kdo) linked to lipid A is a basic constituent of all LPS and Abbreviations. Dhd-7-P, 3-deoxy-~-arabino-2-heptulosonate-7-phosphate; Kdo, 3-deoxy-~-manno-2-octulosonate ; Kdo-k-P, 3-deoxy-D-manno-2-octulosonate-8-phosphate; LPS, lipopolysaccharide.Enzymes. 3-Deoxy-~-arabino-2-heptulosonate-7-phosphate synthase (EC 4.1.2.15); 3-deoxy-~-munno-2-octulosonate-8-phosphate synthase (EC 4.1.2.1 6 ) ; CTP: 3-deoxy-D-matmo-2-octu~osonate cytidylyltransferase, CMP-Kdo synthetase (EC 2.7.7.38); 3-deoxy-~-mannu-2-octulosonate transferase (EC

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“…l). The overall G+C content of the cloned DNA was calculated to 40.1 mo1/100 mol which was in good agreement with 39.6 mol/l00 mol published for C. psittaci (Fukushi and Hirai, 1993). …”

Section: Resultssupporting

confidence: 84%

“…Chlamydiae are obligate intracellular, pathogenic, gramnegative bacteria which are responsible for a variety of human and non human diseases (Schachter, 1988;Grayston et al, 1990;Fukushi and Hirai, 1993). They possess a unique life cycle which is characterized by the interconversion of two distinct morphological forms.…”

mentioning

confidence: 99%

Molecular Cloning, Sequence Analysis and Functional Characterization of the Gene Kdsa, Encoding 3‐Deoxy‐D‐Manno‐2‐Octulosonate‐8‐Phosphate Synthase of Chlamydia Psittaci 6BC

Brabetz¹,

Brade²

1997

European Journal of Biochemistry

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“…This species consists of a group of ruminant strains associated with infectious polyarthritis, encephalitis, pneumonia, and diarrhea (10,11). Studies reporting that DNA-DNA similarity between C. pecorum and strains of other Chlamydia species is less than 10% (10), immunological specificity of the major outer membrane protein (MOMP) gene (10), and dendograms based on multistrain comparison of the MOMP gene sequence (25) have supported the classification of this new species C. pecorum.…”

mentioning

confidence: 76%

Evolutionary relationships among members of the genus Chlamydia based on 16S ribosomal DNA analysis

et al. 1997

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Nucleotide sequences from strains of the four species currently in the genus Chlamydia, C. pecorum, C. pneumoniae, C. psittaci, and C. trachomatis were investigated. In vitro-amplified RNA genes of the ribosomal small subunit from 30 strains of C. pneumoniae and C. pecorum were subjected to solid-phase DNA sequencing of both strands. The human isolates of C. pneumoniae differed in only one position in the 16S rRNA gene, indicating genetic homogeneity among these strains. Interestingly, horse isolate N16 of C. pneumoniae was found to be closely related to the human isolates of this species, with a 98.9% nucleotide similarity between their 16S rRNA sequences. The type strain and koala isolates of C. pecorum were also found to be very similar to each other, possessing two different 16S rRNA sequences with only one-nucleotide difference. Furthermore, the C. pecorum strains truncated the 16S rRNA molecule by one nucleotide compared to the molecules of the other chlamydial species. This truncation was found to result in loss of a unilaterally bulged nucleotide, an attribute present in all other eubacteria. The phylogenetic structure of the genus Chlamydia was determined by analysis of 16S rRNA sequences. All phylogenetic trees revealed a distinct line of descent of the family Chlamydiaceae built of two main clusters which we denote the C. pneumoniae cluster and the C. psittaci cluster. The clusters were verified by bootstrap analysis of the trees and signature nucleotide analysis. The former cluster contained the human isolates of C. pneumoniae and equine strain N16. The latter cluster consisted of C. psittaci, C. pecorum, and C. trachomatis. The members of the C. pneumoniae cluster showed tight clustering and strain N16 is likely to be a subspecies of C. pneumoniae since these strains also share some antigenic cross-reactivity and clustering of major outer membrane protein gene sequences. C. psittaci and strain N16 branched early out of the respective cluster, and interestingly, their inclusion bodies do not stain with iodine. Furthermore, they also share less reliable features like normal elementary body morphology and plasmid content. Therefore, the branching order presented here is very likely a true reflection of evolution, with strain N16 of the species C. pneumoniae and C. psittaci forming early branches of their respective cluster and with C. trachomatis being the more recently evolved species within the genus Chlamydia.

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“…C. abortus is zoonotic, and although most human infections are mild and o�en unnoticed, pregnant women can develop severe, life threatening illness and abort (Jorgensen, 1997). C. pecorum is commonly isolated from the digestive tract of ruminants, and it is responsible for a variety of disease syndromes including conjunctivitis and arthritis in sheep and goats (Fukushi and Hirai, 1993;Storz and Kaltenboeck, 1993).…”

mentioning

confidence: 99%

Seroprevalence of antibodies to chlamydophila abortus shown in awassi sheep and local goats in jordan

Al-Qudah¹,

Sharif²,

Raouf³

et al. 2004

Vet. Med.

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ABSTRACT:A cold complement fixation test (CFT) was used to identify C. abortus infection in ewes and does in northern Jordan. Sera from 36 flocks of sheep and 20 flocks of goats were collected randomly. The results showed that 433 (21.8%) out of 1 984 ovine sera, and 82 (11.4%) out of 721 caprine sera, were seropositive for C. abortus infection, as indicated by a titre ≥ 1:40. However, all the tested sheep flocks and goat flocks (100%) revealed at least one seropositive animal. There was a strong association (P < 0.05) between the rate of C. abortus infection and the size of the sheep flock, when larger flocks had higher infection rates. However, in goats, the flock size had no relationship with seropositivity. Age had no significant (P > 0.05) impact on C. abortus seropositivity. In sheep, there was a significant difference (P < 0.05) between the rates of the chlamydial infection in the four studied areas of northern Jordan. The highest infection rate in sheep (31.2%) was recorded in Mafraq area, while the rates in Irbid, Ajloun and Jerash were 18.5%, 11.2% and 13.9%, respectively. In goats, there was no significant difference between the rates of the chlamydial infection in the two areas studied. The rates of goat infections were 10.8% and 11.8% in Ajloun and Jerash areas, respectively.

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Chlamydia pecorum the Fourth Species of Genus Chlamydia

Cited by 19 publications

References 14 publications

Molecular Cloning, Sequence Analysis and Functional Characterization of the Gene Kdsa, Encoding 3‐Deoxy‐D‐Manno‐2‐Octulosonate‐8‐Phosphate Synthase of Chlamydia Psittaci 6BC

Molecular Cloning, Sequence Analysis and Functional Characterization of the Gene Kdsa, Encoding 3‐Deoxy‐D‐Manno‐2‐Octulosonate‐8‐Phosphate Synthase of Chlamydia Psittaci 6BC

Evolutionary relationships among members of the genus Chlamydia based on 16S ribosomal DNA analysis

Seroprevalence of antibodies to chlamydophila abortus shown in awassi sheep and local goats in jordan

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