Serotyping of Campylobacter jejuni isolated from sporadic cases and outbreaks in British Columbia

Mcmyne, P. M. S.; Penner, J L; Mathias, Richard G.; Black, W. A. P.; Hennessy, J N

doi:10.1128/jcm.16.2.281-285.1982

Cited by 36 publications

(17 citation statements)

References 21 publications

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“…There is a broad agreement among data from developed countries that HS 2 and 4 are the serotypes most often associated with human disease (McMyne et al. 1982; Jones et al.…”

Section: Serotypingmentioning

confidence: 99%

The occurrence of Campylobacter subtypes in environmental reservoirs and potential transmission routes

et al. 2005

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Aim: To identify potential reservoirs and transmission routes of human pathogenic Campylobacter spp. Methods and Results: An enrichment PCR method for the detection and identification of Campylobacter jejuni and/or Campylobacter coli in faecal, food and river water samples was applied to 1450 samples of 12 matrix types obtained from a defined geographical area. PCR-positive samples were cultured to yield isolates for typing, and the data for 616 C. jejuni isolates obtained. Serotyping and SmaI macrorestriction profiling using pulsed field gel electrophoresis revealed a high level of diversity within the isolates from each matrix. Campylobacter jejuni and C. coli subtypes indistinguishable from those obtained from human cases were detected in most of the matrices examined. No Campylobacter isolates were isolated from possum faeces. Conclusions: Ten of the 12 matrices examined may be involved in the transmission of human campylobacteriosis as they contained Campylobacter subtypes also isolated from clinical cases. Significance and Impact of the Study: Results indicate that, for this rural population, a range of potential transmission routes that could lead to campylobacteriosis exist. Their relative importance needs to be assessed from an exposure assessment standpoint.

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“…There is a broad agreement among data from developed countries that HS 2 and 4 are the serotypes most often associated with human disease (McMyne et al. 1982; Jones et al.…”

Section: Serotypingmentioning

confidence: 99%

The occurrence of Campylobacter subtypes in environmental reservoirs and potential transmission routes

et al. 2005

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“…In various studies, the occurrence of O:19 strains causing uncomplicated gastrointestinal infection has been estimated at 1 to 6% (3,44,75,102). Serotype O:19 has also been found in laboratory animals, including dogs, cats, and primates (158).…”

Section: Campylobacter Serotypes Associated With Gbsmentioning

confidence: 99%

CampylobacterSpecies and Guillain-Barré Syndrome

1998

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SUMMARY Since the eradication of polio in most parts of the world, Guillain-Barré syndrome (GBS) has become the most common cause of acute flaccid paralysis. GBS is an autoimmune disorder of the peripheral nervous system characterized by weakness, usually symmetrical, evolving over a period of several days or more. Since laboratories began to isolate Campylobacter species from stool specimens some 20 years ago, there have been many reports of GBS following Campylobacter infection. Only during the past few years has strong evidence supporting this association developed. Campylobacter infection is now known as the single most identifiable antecedent infection associated with the development of GBS. Campylobacter is thought to cause this autoimmune disease through a mechanism called molecular mimicry, whereby Campylobacter contains ganglioside-like epitopes in the lipopolysaccharide moiety that elicit autoantibodies reacting with peripheral nerve targets. Campylobacter is associated with several pathologic forms of GBS, including the demyelinating (acute inflammatory demyelinating polyneuropathy) and axonal (acute motor axonal neuropathy) forms. Different strains of Campylobacter as well as host factors likely play an important role in determining who develops GBS as well as the nerve targets for the host immune attack of peripheral nerves. The purpose of this review is to summarize our current knowledge about the clinical, epidemiological, pathogenetic, and laboratory aspects of campylobacter-associated GBS.

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“…Thermostable antigens extracted from C. jejuni and C. coli have been used in PHA to differentiate strains involved in epidemiological investigations (2,24,37). These antigens were believed to be LPS or 0 antigens because of their biological properties (34).…”

Section: Discussionmentioning

confidence: 99%

Basis for serological heterogeneity of thermostable antigens of Campylobacter jejuni

Mills¹,

Bradbury²,

Penner³

1985

Infect Immun

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Lipopolysaccharides (LPS) were extracted from eight strains of Campylobacterjejuni and purified by enzyme treatment to remove traces of RNA, DNA, and protein. This material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype C. jejuni. The results confirmed that the thermostable antigen typing scheme is based on LPS (0) antigens. The LPS after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to consist of a series of slow migrating bands which could not be elinminated by treatment with NaOH, urea, or EDTA. However, the use of LPS double labeled with 14C and 32p yielded evidence that the bands of high molecular weight were indeed aggregations of low-molecular-weight LPS molecules.Campylobacter jejuni and Campylobacter coli have become recognized as major causes of human bacterial enteritis (40, 41) and interest in the epidemiology of infections due to these organisms has led to the development of serotyping systems. Schemes that differentiate strains through differences in the specificities of surface antigens (13), thermolabile antigens (21), and thermostable antigens (19, 35) have been described. Currently, attention is being directed towards characterizing the biological and biochemical properties of the antigens to seek insight into the basis for the extensive serological heterogeneity noted to occur among strains of these species. At the outset, the thermostable antigens were thought to be lipopolysaccharide (LPS), the somatic 0 antigens common to gram-negative species (34), and investigations at the molecular level that support this view have recently been forthcoming (22,(27)(28)(29). Although the LPS of C. jejuni and C. coli appear similar to LPS of the Enterobacteriaceae in that both have considerable serological heterogeneity, it appears that, structurally, LPS in C. jejuni and C. coli may be quite unique. In the present report, enzyme-purified LPS was used to study the serological specificity of the 0 antigen by passive (indirect) hemagglutination (PHA) and to characterize the structure by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. Evidence is provided to show that the thermostable antigen typing scheme is based on 0 antigens composed of LPS which have structural features different from the LPS of other gram-negative bacterial species. MATERIALS AND METHODSBacterial strains and cultural conditions. The strains of C. jejuni used in this study are listed in Table 1. Also, the smooth strain of Escherichia coli O:111B4 and the Ra (SL3749) and Rc (SL3748) mutants of Salmonella typhimurium were used.Stock cultures were maintained at -70°C in 15% glycerol-I% proteose peptone no. 3 (Difco Laboratories, Detroit, Mich.) and, when required, strains were thawed, plated on brucella agar (Difco), and incubated for 48 h under

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Serotyping of Campylobacter jejuni isolated from sporadic cases and outbreaks in British Columbia

Cited by 36 publications

References 21 publications

The occurrence of Campylobacter subtypes in environmental reservoirs and potential transmission routes

The occurrence of Campylobacter subtypes in environmental reservoirs and potential transmission routes

CampylobacterSpecies and Guillain-Barré Syndrome

Basis for serological heterogeneity of thermostable antigens of Campylobacter jejuni

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