Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes

Rosen, B I; Parwani, Anil V.; López, Susana; Flores, Jorge; Saif, Linda J.

doi:10.1128/jcm.32.2.311-317.1994

Cited by 26 publications

(10 citation statements)

References 24 publications

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“…In addition, porcine rotaviruses with G1 and G2 specificities, which had previously been found exclusively in human rotaviruses, were recently isolated (2,4). By VP4 sequence analysis of porcine rotaviruses (11,14,18,19,25,32), it has been found that a porcine strain, strain Gottfried, shares its VP4 genotype (or P serotype) with human strains recovered from individuals with asymptomatic infections (11,19). These findings highlight the presence of an interspecies relationship of rotaviruses in view of rotavirus ecology.…”

mentioning

confidence: 77%

“…In extensive serological and genomic studies, multiple G serotypes and P serotypes have been recognized in a given species. In porcine rotaviruses, four G serotypes (G3, G4, G5, and G11) have been identified since the first detection of the rotavirus in piglets in 1975 (1,3,5,15,17,23,24,26,28,30,32). In addition, porcine rotaviruses with G1 and G2 specificities, which had previously been found exclusively in human rotaviruses, were recently isolated (2,4).…”

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confidence: 99%

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Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus

et al. 1996

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A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty-three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively. Two samples exhibited two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10, respectively. For one specimen, containing a serotype G10 virus (strain P343), virus was isolated in MA-104 cells, and the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotype (or VP4 genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.

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confidence: 77%

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confidence: 99%

Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus

et al. 1996

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“…Epidemiological information related to the prevalence and genotype specificities of porcine GARVs are beneficial for the development of effective vaccines (Rosen et al, 1994). Therefore, we investigated the prevalence of porcine GARV infections as well as their genotype diversities in South Korea.…”

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confidence: 99%

Detection and genotyping of Korean porcine rotaviruses

Kim

Park

et al. 2010

Veterinary Microbiology

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Porcine group A rotavirus (GARV) is considered to be an important animal pathogen due to their economic impact in the swine industry and its potential to cause heterologous infections in humans. This study examined 475 fecal samples from 143 farms located in 6 provinces across South Korea. RT-PCR and nested PCR utilizing primer pairs specific for the GARV VP6 gene detected GARV-positive reactions in 182 (38.3%) diarrheic fecal samples. A total of 98 porcine GARV strains isolated from the GARV-positive feces were analyzed for G and P genotyping. Based on the sequence and phylogenetic analyses, the most predominant combination of G and P genotypes was G5P[7], found in 63 GARV strains (64.3%). The other combinations of G and P genotypes were G8P[7] (16 strains [16.3%]), G9P[7] (7 strains [7.1%]), G9P[23] (2 strains [2.0%]), and G8P[1] (1 strain [1.0%]). The counterparts of G or P genotypes were not determined in three G5, five P[7], and one P[1] strains. Interestingly, phylogenetic analysis indicated that all Korean G9 strains were more closely related to lineage VI porcine and human viruses than to other lineages (I-V) of GARVs and to Korean human G9 strains (lineage III). These results show that porcine GARV infections are common in diarrheic piglets in South Korea. The infecting strains are genetically diverse, and include homologous (G5P[7]), heterologous (G8P[1]), and reassortant (G8P[7]), as well as emerging G9 GARV strains.

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“…Porcine rotaviruses are the major cause of acute diarrhea in the piglets [3,4] and can cause mild-severe diarrhea associated with potentially high morbidity and mortality. Group A rotaviruses cause diarrhea in pigs both before and after weaning [5] and can account for 53 and 44% pre-and post-weaning rotavirus-associated diarrhea in swine, respectively [6].…”

Section: Introductionmentioning

confidence: 99%

Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice

Qiao

Wang

et al. 2009

BMC Microbiol

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BackgroundPorcine rotavirus infection is a significant cause of morbidity and mortality in the swine industry necessitating the development of effective vaccines for the prevention of infection. Immune responses associated with protection are primarily mucosal in nature and induction of mucosal immunity is important for preventing porcine rotavirus infection.ResultsLactobacillus casei expressing the major protective antigen VP4 of porcine rotavirus (pPG612.1-VP4) or VP4-LTB (heat-labile toxin B subunit from Echerichia coli) (pPG612.1-VP4-LTB) fusion protein was used to immunize mice orally. The expression of recombinant pPG612.1-VP4 and pPG612.1-VP4-LTB was confirmed by SDS-PAGE and Western blot analysis and surface-displayed expression on L. casei was verified by immunofluorescence. Mice orally immunized with recombinant protein-expressing L. casei produced high levels of serum immunoglobulin G (IgG) and mucosal IgA. The IgA titters from mice immunized with pPG612.1-VP4-LTB were higher than titters from pPG612.1-VP4-immunized mice. The induced antibodies demonstrated neutralizing effects on RV infection.ConclusionThese results demonstrated that VP4 administered in the context of an L. casei expression system is an effective method for stimulating mucosal immunity and that LTB served to further stimulate mucosal immunity suggesting that this strategy can be adapted for use in pigs.

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Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes

Cited by 26 publications

References 24 publications

Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus

Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus

Detection and genotyping of Korean porcine rotaviruses

Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice

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