Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus

Abstract

A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty-three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively. Two samples exhibited two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10, respectively. For one specimen, containing a serotype G10 virus (strain P343), virus was isolated in MA-104 cells, and the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotype (or VP4 genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.

Verification of Sensitivity and Specificity of Group A Rotavirus Detection in Piglets Faeces with Monoclonal Blocking ELISA Methods

“…The fact that all groups of rotavirus are detected by EM, while group A rotavirus only is detected by CB-ELISA, can explain this difference. The congruency of EM and CB-ELISA examinations (73.1%) indicates the share of rotavirus A in cases of rotaviral gastroenteritis and correlates with other findings (Sigolo de San et al, 1986;Janke et al, 1990;Pongsuwanna et al, 1996). The assumption that the remaining seven (26.9%) samples likely contain non-group A rotavirus was partly confirmed by demonstration of group C rotavirus electropherotype in one EM highly positive sample.…”

“…Seven antigenically distinct groups of rotaviruses (A-G) were described; four (A, B, C and E) of them are pathogenic for pigs. Group A rotavirus which is the most often (67.8-95.4%) causal agent of rotaviral gastroenteritis (Sigolo de San et al, 1986;Janke et al, 1990;Pongsuwanna et al, 1996) comprises a series of subgroups and serotypes according to antigenic analysis of Vp4 and Vp7 proteins present in the outer capsid of virion. Common group A rotavirus Vp6 antigen is found in the inner capsid of virion, and rotaviral infections caused by that agent may be diagnosed by use of specific Vp6 antibodies regardless of animal species affected.…”

Detection and molecular characterization of porcine group C rotaviruses in South Korea

“…There is now increasing evidence that the transmission of group A rotaviruses can occur from animal-to-human as well as from animal-to-animal by direct transmission of the virus or by the contribution of one or several genes to reassortants (Ghosh et al, 2007;Griffin et al, 2002;Martella et al, 2006;Matthijnssens et al, 2006;Palombo et al, 2000;Pongsuwanna et al, 1996). Compared to group A rotaviruses, there is a paucity of information regarding the sequence and phylogenetic data on all 11 genomic segments of GCRVs.…”

Characterization of human rotavirus strains with G12 and P[9] detected in Japan

“…Northern blot hybridization was carried out as described previously [Pongsuwanna et al, 1996]. In brief, after PAGE analysis, dsRNA was denatured by soaking the gel in 0.1 N NaOH and 0.25 M NaCl for 20 min, and then neutralized in 4Â Tris-acetate-EDTA (TAE) for 20 min twice and in 1Â TAE for 20 min.…”