Serotypes of Ureaplasma urealyticum in spontaneous abortion

Robertson, Janet A.; Honoré, Louis H.; Stemke, Gerald W.

doi:10.1097/00006454-198611010-00014

Cited by 40 publications

(41 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First we examined the DNA from 18 coded, previously serotyped isolates of the abortion study (19). Their biovars had been predicted based on experience with serotyping, and more recently the isolates had been typed to the biovar level by 16S rRNA gene PCRs (23).…”

Section: Resultsmentioning

confidence: 99%

“…Eighteen isolates from tissues of subjects who had experienced abortion, whose serovars had been previously established using polyclonal antiserum and a colony epifluorescence method (19,25), were cultured in broth, and the cells were centrifuged and resuspended in 70% ethanol until the DNA was extracted. The preparations used for PCR-SSCP analyses were later passages than those used for serotyping.…”

Section: Strainsmentioning

confidence: 99%

“…Robertson et al (19) were unable to ascribe any correlation between serovars isolated from tissues of subjects experiencing spontaneous and therapeutic abortion.…”

mentioning

confidence: 97%

See 2 more Smart Citations

Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single-Strand Conformation Polymorphism Analysis

Pitcher¹,

Sillis

Robertson

2001

J Clin Microbiol

Self Cite

View full text Add to dashboard Cite

Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Using a commercially available precast DNA mutation detection gel system, we have developed a simple and reproducible PCR-single-strand conformation polymorphism analysis method for differentiating the biovars of this species that reveals five patterns among the 14 serovars and enables clinical isolates to be typed directly from broth cultures.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Strainsmentioning

confidence: 99%

See 1 more Smart Citation

Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single-Strand Conformation Polymorphism Analysis

Pitcher¹,

Sillis

Robertson

2001

J Clin Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Human ureaplasmas are recognized causes of urethritis (Hewish et al, 1986 ;Cracea et al, 1985 ;TaylorRobinson et al, 1985), and have been associated with complications of pregnancy and prematurity (AbeleHorn et al, 1997 ;Robertson et al, 1986 ;Kundsin et al, 1996 ;Hannaford et al, 1999). However, as common genital tract commensals (Viarengo et al, 1980 ;Cracea et al, 1985), their pathogenic roles in individual cases are difficult to confirm (Zheng et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Molecular genotyping of human Ureaplasma species based on multiple-banded antigen (MBA) gene sequences.

Kong¹,

Ma²,

James³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Ureaplasma urealyticum has been divided into 14 serovars. Recently, subdivision of U. urealyticum into two species has been proposed : U. parvum (previously U. urealyticum parvo biovar), comprising four serovars (1, 3, 6, 14) and U. urealyticum (previously U. urealyticum T-960 biovar), 10 serovars (2, 4, 5, 7-13). The multiple-banded antigen (MBA) genes of these species contain both species and serovar/subtype specific sequences. Based on whole sequences of the 5'-ends of MBA genes of U. parvum serovars and partial sequences of the 5'-ends of MBA genes of U. urealyticum serovars, we previously divided each of these species into three MBA genotypes. To further elucidate the relationships between serovars, we sequenced the whole 5'-ends of MBA genes of all 10 U. urealyticum serovars and partial repetitive regions of these genes from all serovars of U. parvum and U. urealyticum. For the first time, all four serovars of U. parvum were clearly differentiated from each other. In addition, the 10 serovars of U. urealyticum were divided into five MBA genotypes, as follows : MBA genotype A comprises serovars 2, 5, 8 ; MBA genotype B, serovar 10 only ; MBA genotype C, serovars 4, 12, 13 ; MBA genotype D, serovar 9 only ; and MBA genotype E comprises serovars 7 and 11. There were no sequence differences between members within each MBA genotype. Further work is required to identify other genes or other regions of the MBA genes that may be used to differentiate U. urealyticum serovars within MBA genotypes A, C and E. A better understanding of the molecular basis of serotype differentiation will help to improve subtyping methods for use in studies of the pathogenesis and epidemiology of these organisms.

show abstract

“…It has been suggested that only some of the 14 known serotypes of U. urealyticum may be pathogenic and that the others are not involved in disease (Robertson et al, 1986;Cassell et al, 1987;Naessens et al, 1988). To establish the pathogenic potential of individual serotypes requires identification of serotype-specific antigens.…”

Section: Introductionmentioning

confidence: 99%

Isolation and purification of viable Ureaplasma urealyticum cells free from medium components

Horowitz

Gal

1991

Journal of General Microbiology

View full text Add to dashboard Cite

A procedure was devised to produce Ureaplasma uredyticum preparations free of adsorbed components of the growth medium, which contains high concentrations of serum. The ureaplasmas were cultivated in a medium containing PPLO-serum fraction as a replacement for horse serum. High titres of ureaplasmas (> lo7 c.f.u. ml-l) were obtained. Harvested cells were then purified by Urograh density gradient centrifugation. By use of 3H-labelled ureaplasma cells and 251-labelled medium components, a distinct band of viable cells devoid of serum constituents was demonstrated. The absence of medium components was verified by immunoblotting cells from this band with antiserum to medium components. Medium components that had been present before the purification procedure were undetectable in the purified cell fraction obtained. The viability of the purified ureaplasma cells represented an 85 % recovery rate and their antigenicity, examined with anti-serotype specific antiserum, remained intact. This easy and reproducible procedure can be used to prepare purified ureaplasmas for investigation of ureaplasmal antigens and their expression and/or role in disease.

show abstract

Serotypes of Ureaplasma urealyticum in spontaneous abortion

Cited by 40 publications

References 0 publications

Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single-Strand Conformation Polymorphism Analysis

Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single-Strand Conformation Polymorphism Analysis

Molecular genotyping of human Ureaplasma species based on multiple-banded antigen (MBA) gene sequences.

Isolation and purification of viable Ureaplasma urealyticum cells free from medium components

Contact Info

Product

Resources

About