Serotypes of Escherichia coli isolated from healthy infants in Berlin, Germany and Melbourne, Australia

Bettelheim, K. A.; Beutin, Lothar; Gleier, Kerstin; Pearce, J.L.; Luke, R. K. J.; Zimmermann, Sabine

doi:10.1016/s0147-9571(02)00015-2

Cited by 8 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fecal specimens from randomly selected infants less than 2 years old, who had not had any gastrointestinal symptoms for at least 1 week prior to the collection of the specimen and who were currently not taking any antibiotic therapy, were collected in Berlin, Germany, between 1986 and 1987 (205 infants) and Melbourne, Australia, between 1988 and 1994 (184 infants) (5).…”

Section: Infantsmentioning

confidence: 99%

HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing Escherichia coli Strains Isolated from Healthy Infants in Germany and Australia

et al. 2003

View full text Add to dashboard Cite

Fecal samples from healthy children under 2 years of age living in Berlin, Germany (205 infants), and Melbourne, Australia (184 infants), were investigated for the presence of attaching and effacing (AE) Escherichia coli (AEEC) strains by screening for eae (intimin) genes. Twenty-seven AEEC strains were isolated from 14 children (7.6%) from Melbourne and from 12 children (5.9%) from Berlin. The 27 AEEC strains were classified as enterohemorrhagic E. coli (one strain, producing Shiga toxin 1), typical enteropathogenic E. coli (EPEC) (one strain carrying an EPEC adherence factor [EAF] plasmid), and atypical EPEC (25 strains negative for Shiga toxins and EAF plasmids). The AEEC were divided into 18 different serotypes, O-nontypeable and O-rough strains. Typing of their intimin genes revealed the presence of intimin ␣ in 6 strains, intimin ␤ in 11 strains, intimin ␥ in 7 strains, intimin in 2 strains, and intimin in one strain. Analysis of HEp-2 cell adherence showed diffuse adherence or localized adherence-like patterns in 26 AEEC strains; local adherence was found only with the EAF-positive strain. Ten AEEC strains showed an AE property with the fluorescent actin staining (FAS) test. The introduction of an EAF plasmid (pMAR7) converted 11 FAS-negative AEEC strains to FAS positive and increased the FAS reaction in six FAS-positive AEEC strains, indicating that the genes needed for the AE phenotype were functional in these strains. Our finding indicates that atypical EPEC strains could play a double role as strains that naturally immunize against intimin in humans and as reservoirs for new emerging human pathogenic EPEC strains.The ability to cause attaching and effacing (AE) lesions in cells of the intestinal mucosa was identified as an important pathogenicity factor of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) strains (42,64). The AE lesion is generated by intimate attachment of bacteria to the enterocytes, which is followed by aggregation of the cytoskeletal actin and effacement of microvilli (34). Actin aggregation caused by AE E. coli (AEEC) strains results in a typical picture which can be visualized by a fluorescent actin staining (FAS) test which is used for detection of EPEC and EHEC (34,35,58). The genes coding for the AE lesion are located on a pathogenicity island termed "locus of enterocyte effacement" (LEE) in EPEC and EHEC strains. The 34-kb core region of the LEE is highly conserved in human and animal EPEC and EHEC strains and contains polycistronic operons harboring genes for type III secretion and adhesion proteins (18,40,51,68). Intimate attachment of bacteria to the eucaryotic cell is mediated by intimin, the product of the eae gene, which is incorporated in the bacterial membrane. Intimin binds to the translocated intimin receptor (Tir), which is presented on the surface of the eucaryotic target cell (for a review, see reference 64). The detection of the intimin gene was taken as an indicator for the presence of functional LEE genes because it was shown that ...

show abstract

Section: Infantsmentioning

confidence: 99%

HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing Escherichia coli Strains Isolated from Healthy Infants in Germany and Australia

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Certain serotypes of E coli are more associated with some of virulence factors than the others. 13 Recent studies have showed that the restriction analysis of fliC gene (encoding flagelin) can be used to type motile and nonmotile E coli serotypes. 14e16 Although in our previous study 17 the association of atypical EPEC serogroups with diarrhea was statistically significant, more characterization of the strains are needed to elucidate the role of atypical EPEC serotypes in diarrheal diseases among Iranian children.…”

Section: Introductionmentioning

confidence: 99%

Molecular and phenotypic characterization of atypical enteropathogenic Escherichia coli serotypes isolated from children with and without diarrhea

Aslani¹,

Alikhani²

2011

Journal of Microbiology, Immunology and Infection

View full text Add to dashboard Cite

show abstract

“…Rivero et al (2010) reported flagellar types H7, H -, H11, H19 and H2 in diarrhoeic infants, whereas Ge et al (2002) reported flgellar types H11, H7 and NM in diarrhoeic infants. A wide range of of flagellar types in diarrhoeic and non diarrhoeic infants were also reported by various others workers (Rehua-Mangia et al, 2004;Bettelheim et al, 2003;Jenkins et al, 2003). Schierack et al (2006) recorded the prevalence of flagellar types H6, H -, H10 and H4 in pigs.…”

Section: Discussionmentioning

confidence: 52%

Molecular and phenotypic characterization of shigatoxigenic Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) from piglets and infants associated with diarrhoea in Mizoram, India

Begum¹,

Dutta²,

Chandra³

et al. 2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Limited information is available on shiga toxin producing Escherichia coli (STEC) in pigs and infants from Aizawl, Mizoram and North Eastern region of India. This cross sectional study was conducted on faecal samples from pigs and infants to detect and characterize of STEC and enteropathogenic E. coli (EPEC). Serogrouping, molecular and phenotypic characterizations were done by standard molecular and cytotoxic assays. Out of 48 E. coli strains isolated from 320 diarrhoeic fecal samples of piglets, 44 belonged to 18 different serogroups, 3 (6.25%) were untypeable (UT) and 1(2.08%) was recorded as rough strain (R). Similarly, out of 17 E. coli strains isolated from 264 diarrhoeic fecal samples of infants, 16 belonged to O60 (94.1%) and 1(5.88%) was untypeable. Virulence genes (stx1, stx2, eaeA and hlyA) were detected by multiplex PCR assay. A total of 1260 E. coli were isolated from piglets (720) and infants (540) from 584 faecal samples. All together, 5.16% (65) E. coli isolates were found to be positive for at least one virulence gene (6.66% piglets and 3.15% infants). Out of the virulent gene positive E. coli 3.17% (32 from piglets and eight from infants) and 1.98% (16 from piglets and nine from infants) were recorded as STEC and EPEC, respectively. On the other hand, from the total 2.14% stx 2 positive isolates, 16 and 11 were positive for stx 2e and stx 2c subtypes, respectively. Similarly, from the 4.04% eaeA positive isolates, 1.19% (15) were positive for bfpA gene, of which 1.67% (12) were piglets and 0.60% (3) were infants. All the isolates were exhibited varying degree of CPE on vero cell lines. In conclusion, STEC and EPEC seem to be associated with diarrhoea in piglets and infants in Mizoram. In piglets STEC strains represent as a major cause of diarrhoea while EPEC strains represent as major cause of diarrhoea in infants in North Eastern region of the India.

show abstract

Serotypes of Escherichia coli isolated from healthy infants in Berlin, Germany and Melbourne, Australia

Cited by 8 publications

References 12 publications

HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing Escherichia coli Strains Isolated from Healthy Infants in Germany and Australia

HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing Escherichia coli Strains Isolated from Healthy Infants in Germany and Australia

Molecular and phenotypic characterization of atypical enteropathogenic Escherichia coli serotypes isolated from children with and without diarrhea

Molecular and phenotypic characterization of shigatoxigenic Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) from piglets and infants associated with diarrhoea in Mizoram, India

Contact Info

Product

Resources

About