Molecular and phenotypic characterization of atypical enteropathogenic Escherichia coli serotypes isolated from children with and without diarrhea

Aslani, Mohammad Mehdi; Alikhani, Mohammad Yousef

doi:10.1016/j.jmii.2011.01.006

Cited by 8 publications

(10 citation statements)

References 26 publications

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“…Animals can be reservoirs of atypical EPEC, in contrast to typical EPEC, in which humans are the sole reservoir [61], indicating that food chain and animal contact could be other factors for the dissemination of resistant DEC among pediatrics in Qatar. In contrast with our findings, studies from Iraq and India documented atypical EPEC harboring bfpA without eaeA gene [34,56]. Early studies conducted in England and Peru have shown that atypical EPEC is often found in children with and without diarrhea, and the pathogenic potential of atypical EPEC strains has been speculative in the past [62,63].…”

Section: Discussioncontrasting

confidence: 99%

“…Similar to our findings, in the MENA region, atypical EPEC strains have been the most manifested in Iran (100%), Iraq (66.7%) and Kuwait (95.6%) [34,55,56]. They have been also reported in other countries worldwide, such as Brazil, North-West Italy, Melbourne, India [56][57][58][59][60]. In agreement with our findings, the atypical EPEC organisms that possess eae alone have been reported to be more prevalent in both developing and developed countries.…”

Section: Discussionsupporting

confidence: 91%

“…All 56 EPEC isolates were atypical, harboring only eaeA (absence of bfpA gene), whereas in typical EPEC both genes eaeA and bfpA are present [22,54]. Similar to our findings, in the MENA region, atypical EPEC strains have been the most manifested in Iran (100%), Iraq (66.7%) and Kuwait (95.6%) [34,55,56]. They have been also reported in other countries worldwide, such as Brazil, North-West Italy, Melbourne, India [56][57][58][59][60].…”

Section: Discussionsupporting

confidence: 87%

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Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar

et al. 2020

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Background: The treatment of Enterobacteriaceae family including diarrheagenic E. coli (DEC) has been increasingly complicated due to the emergence of resistant strains. Here we report on the phenotypic resistance profiles and ESBL genotype and virulence profiles of Enteroaggregative E. coli (EAEC) and Enteropathogenic E. coli (EPEC) isolated from children hospitalized with acute gastroenteritis in Qatar (AGE). Results: E. coli were isolated and characterized from 76 diarrheagenic stool positive samples, collected from hospitalized children less than 10 years old. Isolates were tested for antibiotic susceptibility against eighteen clinically relevant antibiotics using E-test method. Conventional PCR was performed to detect genes encoding ESBL and virulence factors. Chi-square test was performed to compare the individual antibiotic resistance between EPEC and EAEC. A significant percentage (73.7%) of isolates were resistant to at least one antibiotic. Overall, high resistance (70%) was reported to the first-line antibiotics such as ampicillin, tetracycline (46.4%), and sulfamethoxazole-trimethoprim (42.9%). Further, 39.5% of the isolates were multidrug resistant (MDR), with 22.4% being ESBL producers. On the other hand, all isolates were susceptible to carbapenem, fosfomycin, amikacin and colistin. The incidences of resistance to the 18 antibiotics between EPEC and EAEC were not significantly different by Pearson chi-square test (P > 0.05). Genetic analysis revealed that 88.23% of ESBL production was bla CTX-M-G1 (bla CTX-M-15 , bla CTX-M-3)encoded. Several different combinations of virulence markers were observed, however, there was no specific trend among the isolates apart from absence of the bundle-forming pilus (bfpA) gene, which encodes the type IV fimbriae in EPEC adherence factor (EAF) plasmid (pEAF), among all EPEC (atypical). 15% of the EAEC strains were positive for a combination of astA, aap & capU, while 10% were positive for three different combinations. The aap, aatA, capU and aggR virulence genes showed the highest frequency of 65, 60, 55 and 55% respectively. Others genes, east, astA, and aai, showed frequencies of 35, 30 and 20% respectively.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

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Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar

et al. 2020

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“…A study from Iraq documented only atypical EPEC harboring bfpA without eaeA gene [ 22 ]. In a study conducted in Iran for both types of atypical EPEC, those harboring only eaeA and those harboring only bfpA were detected [ 23 ]. Another study from India documented the isolation of atypical EPEC (harboring only eaeA gene) from children with diarrhea and atypical EPEC (harboring only bfpA gene) from children without diarrhea in control group [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

A study of virulence and antimicrobial resistance pattern in diarrhoeagenic Escherichia coli isolated from diarrhoeal stool specimens from children and adults in a tertiary hospital, Puducherry, India

Natarajan

Kumar

Mandal

et al. 2018

J Health Popul Nutr

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BackgroundEmergence of atypical enteropathogenic Escherichia coli (EPEC) and hybrid E. coli (harboring genes of more than one DEC pathotypes) strains have complicated the issue of growing antibiotic resistance in diarrhoeagenic Escherichia coli (DEC). This ongoing evolution occurs in nature predominantly via horizontal gene transfers involving the mobile genetic elements like integrons notably class 1 integron. This study was undertaken to determine the virulence pattern and antibiotic resistance among the circulating DEC strains in a tertiary care center in south of India.MethodsDiarrhoeal stool specimens were obtained from 120 children (< 5 years) and 100 adults (> 18 years), subjected to culture and isolation of diarrhoeal pathogens. Conventional PCR was performed to detect 10 virulence and 27 antimicrobial resistance (AMR) genes among the E. coli isolated.ResultsDEC infection was observed in 45 (37.5%) children and 18 (18%) adults, among which [18 (40%), 10 (10%)] atypical EPEC was most commonly detected followed by [6 (13.3%), 4 (4%)] ETEC, [5 (11.1%) 2 (2%)] EAEC, [(3 (6.6%), 0 (0%)] EIEC, [3 (6.6%), 0 (0%] typical EPEC, and [4 (8.8%), 1 (1%)] STEC, and no NTEC and CDEC was detected. DEC co-infection in 3 (6.6%) children, and 1(1%) adult and sole hybrid DEC infection in 3 (6.6%) children was detected. The distribution of sulphonamide resistance genes (sulI, sulII, and sulIII were 83.3 and 21%, 60.41 and 42.1%, and 12.5 and 26.3%, respectively) and class 1 integron (int1) genes (41.6 and 26.31%) was higher in DEC strains isolated from children and adults, respectively. Other AMR genes detected were qnrS, qnrB, aac(6’)Ib-cr, dhfr1, aadB, aac(3)-IV, tetA, tetB, tetD, catI, blaCTX, blaSHV, and blaTEM. None harbored qnrA, qnrC, qepA, tetE, tetC, tetY, ermA, mcr1, int2, and int3 genes.ConclusionsAtypical EPEC was a primary etiological agent of diarrhea in children and adults among the DEC pathotypes. Detection of high numbers of AMR genes and class 1 integron genes indicate the importance of mobile genetic elements in spreading of multidrug resistance genes among these strains.Electronic supplementary materialThe online version of this article (10.1186/s41043-018-0147-z) contains supplementary material, which is available to authorized users.

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“…EHEC was the most detected pathotype found in both human and water samples followed by EPEC/EHEC. These pathotypes were the most detected pathotypes in children with diarrhoea in two studies performed in Iran 7,59 and Spain 16 . EHEC is the primary cause of severe gastroenteritis and HUS outbreak in Germany 60 .…”

Section: Discussion Identification and Distribution Of Dec Pathotypesmentioning

confidence: 83%

Antibiotic Resistance Profile and Clonality of E. coli Isolated from Water and Paediatric Stool Samples in the North-West, Province South Africa

Chukwu¹,

Abia²,

Ubomba-Jaswa³

et al. 2019

J Pure Appl Microbiol

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This study investigated the antibacterial resistance profiles of E. coli pathotypes isolated from children under five years and drinking water samples collected from the North West Province of South Africa and ascertained the clonality of the isolates. Two hundred and forty-one E. coli isolates were recovered from stool samples of diarrhoeic and non-diarrhoeic children under five years old, and drinking water, using the Colilert-18 ® Quanti-tray/2000 and Eosin methylene blue agar. The presence of enteropathogenic (eaeA), enterohaemorrhagic (eaeA,stx1, stx2 and flicH7), enteroaggregative (eagg), enteroinvasive (ipaH) and enterotoxigenic (ST and LT) E. coli pathotypes were also investigated using PCR. Antibiotic susceptibility was carried out through the disk diffusion method. The presence of blaCTX-M, blaSHV, blaCMY, and blaDHA genes that code for β-lactamases was investigated using real-time PCR. Similarities between human and water isolates were tested using ERIC-PCR. Overall, EHEC (35.8%), EPEC/EHEC (22%), ETEC (21.6%) and EIEC (20.2%) were detected. The highest antibiotic resistance was detected to Clarithromycin (100%) and Erythromycin (100%) while the lowest resistance was against Gentamicin (0.4%). Also, 100% sensitivity was recorded to imipenem and meropenem. Multi-antibiotic resistance was observed in all the pathotypes, and the ESBL genes were detected in 71.6% of the pathotypes. ERIC-PCR indicated 100% similarities in some water and human samples. Pathogenic E. coli is amongst the diarrhoea-causing agents in the North West Province with EHEC being the most identified pathotype. The clonal relatedness of the human and water isolates suggests that domestic water might be a route of transmission.

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Molecular and phenotypic characterization of atypical enteropathogenic Escherichia coli serotypes isolated from children with and without diarrhea

Cited by 8 publications

References 26 publications

Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar

Antibiotic resistance and virulence patterns of pathogenic Escherichia coli strains associated with acute gastroenteritis among children in Qatar

A study of virulence and antimicrobial resistance pattern in diarrhoeagenic Escherichia coli isolated from diarrhoeal stool specimens from children and adults in a tertiary hospital, Puducherry, India

Antibiotic Resistance Profile and Clonality of E. coli Isolated from Water and Paediatric Stool Samples in the North-West, Province South Africa

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