Serotonin-Related Gene Variants in Patients with Irritable Bowel Syndrome and Depressive or Anxiety Disorders

Abstract

Aim To assess the association of six polymorphisms in serotonin-related genes with depressive or anxiety disorders in patients with irritable bowel syndrome (IBS). Methods The lifetime prevalence of depressive and anxiety disorders was assessed in 95 IBS patients (85% women) using the Munich version of the Composite International Diagnostic Interview (CIDI). IBS was diagnosed according to the Rome III criteria. SCL6A4 HTTLPR polymorphism (rs4795541) was determined using PCR-based method. Single-nucleotide polymorphisms in HTR1A (rs6295), HTR2A (rs6313 and rs6311), HTR2C (rs6318), and TPH1 (rs1800532) were detected by minisequencing method. Results IBS patients with depressive disorders were characterized by higher frequency of 5-HTTLPR L allele in comparison to IBS patients with anxiety disorders. The lower frequency of 1438 A allele in HTR2A was found in IBS patients with depressive disorders in comparison to IBS patients without mental disorders. The lower G allele frequency in HTR2C rs6318 polymorphism among IBS patients with anxiety disorders was also observed. Conclusions Our results provide further evidence for the involvement of SLC6A4 rs4795541 and HTR2A rs6311 polymorphisms in the pathophysiology of depressive disorders in IBS patients. The new findings indicate that HTR2C rs6318 polymorphism may be associated with the susceptibility to anxiety disorders in IBS patients.

Association Analysis of 14 Candidate Gene Polymorphism with Depression and Stress among Gestational Diabetes Mellitus

“…Genes candidates were selected based on previous data implicating an association with the studies SNPs and clinical syndrome of depression [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41]; anxiety [42][43][44] or stress [45] diagnosed according to Diagnostic and Statistical Manual of Mental Disorders and/or International Statistical Classification of Diseases. The genotyping analysis of candidate genes polymorphism was analyzed using Agene ® MassARRAY platform.…”

“…These genes may be associated with depression or anxiety; however, there are ample studies which have failed to replicate the same results in the candidate gene literature [29][30][31]. One explanation for this lack of success in producing the replicable main effect of these genes is that the certain genetic variants are highly dependent on the gender, population, and disease-related outcomes [32]; even though these studies have recruited patients with major depressive disorder [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41]; anxiety disorder [42][43][44]; and post-traumatic stress disorders [45] diagnosed according to Diagnostic and Statistical Manual of Mental Disorders and/or International Statistical Classification of Diseases. This has led to increasing skepticism about the true association or lack thereof between candidate genes and psychological symptoms of depression, anxiety and/or stress.…”

“…To this end, we have constructed a custom of SNP array containing 18 genes that were chosen based on hypotheses regarding biological systems of relevance to depression [46][47][48][49][50]; anxiety [42,51,52] and stress [45,53]. These custom SNPs provide excellent coverage of many previously suggested and functionally important candidate genes for depression, anxiety and stress, including NPY5R [42,52]; ANO2 [42]; EPHX2 [42,51]; TPH2 [35]; NRG1 [34]; LHPP [38,39,54]; FKBP5 [41,45]; SDK2 [42]; RORA [33,55]; OXTR [27,28]; BDNF [56,57]; HTR2C [43]; TEX51 [42]; and PLEKHG1 [42]. Many of the genes represented on the array have also been reported to be involved in associated heritable phenotypes that are associated with symptoms of depression, anxiety and/or stress.…”

Transplante de microbiota fecal no tratamento da síndrome do intestino irritável: uma revisão sistemática

“…São considerados sintomas clínicos característicos: a dor abdominal, as mudanças no número de evacuações e as mudanças na consistência das fezes (4) . Esta última alteração clínica é classificada de acordo com a escala de Bristol (EB) e é responsável por determinar em qual subgrupo da SII o paciente se encontra, sendo esses subgrupos:…”

“…O diagnóstico da SII é alcançado através dos dados clínicos, tomando por base os novos critérios de Roma IV (4) , o qual exige a presença de um critério obrigatório (dor abdominal frequente, pelo menos 1 vez por semana, mantida nos últimos 3 meses e recorrente há mais de 6 meses) associada a dois ou mais dos seguintes: relação com evacuação (piora ou melhora da dor), mudança nas frequencia das evacuações e alteração na EB. Para confirmação diagnóstica, devem-se realizar os exames orientados pela anamnese e caso haja presença de sinais de alarme, deve ser realizada colonoscopia para excluir causas orgânicas (6) .…”