Serotaxonomical Analyses of Some Streptomyces and Related Organisms

Ridell, Malin; Williams, Stanley T.

doi:10.1099/00221287-129-9-2857

Cited by 12 publications

(8 citation statements)

References 21 publications

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“…The S,,/UPGMA value ranges of the test strains compared to the mean IND-ELISA similarities were as follows : the > 81 % S,,/UPGMA range had a mean IND-ELISA similarity of 79%; the < 81 % to > 77.5 % range had a mean similarity of 71 %; the < 77.5 % to > 70-1 % range had a mean similarity of 43%; while the < 70.5% range had a mean similarity of 34%. There was a strong correlation between the two systems, supporting the conclusions of Ridell & Williams (1983), who used Ouchterlony double diffusion assays. However, the IND-ELISA test is rapid and can be easily mechanised, and should prove to be a more reliable and reproducible system for such studies.…”

Section: R E S U L T S a N D Discussionsupporting

confidence: 73%

“…griseocarneum is classified within the main group of Streptomyces by IND-ELISA although with both antisera it showed a similarity approaching that of the least similar Streptomyces species. Ridell & Williams (1983) found that the two species of Streptouerticillium tested had two precipitin lines in common with a number of Streptomyces species. Thus both techniques indicate that the genera Streptomyces and Streptoverticillium are related but distinct (Williams et al, 1985).…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

“…In previous studies using serological techniques, Ouchterlony double-diffusion assays have been used to measure the major protein similarities of Streptomyces species (Ridell & Williams, 1983). This approach has three disadvantages.…”

Section: Introductionmentioning

confidence: 99%

“…Nucleic acid hybridization (DNA-RNA or DNA-DNA) requires the use of radiochemicals, the careful preparation of nucleic acid and a high degree of control of hybridization conditions (Mordarski et al, 1981). In a few cases, serology has been used to study the taxonomy of Streptomyces and related species with some success (Ridell, 1981;Ridell & Williams, 1983).…”

Section: Introductionmentioning

confidence: 99%

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Enzyme-linked Immunosorbent Assay (ELISA) as a Means of Taxonomic Analysis of Streptomyces and Related Organisms

Kirby¹,

Rybicki²

1986

Microbiology

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Fourteen Streptomyces strains from various numerical taxonomic classes and representatives of three other genera of actinomycetes were studied using an indirect enzyme-linked immunosorbent assay (IND-ELISA) to determine their serological relationships. The IND-ELISA results agreed with those from previous numerical taxonomic analyses and Ouchterlony double-diffusion studies. The IND-ELISA method is quicker, more quantitative and less subjective than Ouchterlony assays and thus should be useful in Streptomyces taxonomy. The results indicated that Frankia sp-CpI 1 was related to Streptomyces.

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Section: R E S U L T S a N D Discussionsupporting

confidence: 73%

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enzyme-linked Immunosorbent Assay (ELISA) as a Means of Taxonomic Analysis of Streptomyces and Related Organisms

Kirby¹,

Rybicki²

1986

Microbiology

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“…The extract was centrifuged at 50,000 X g for 60 min at 4"C, and the supernatant was filtered through a Millipore filter (pore size, 0.45 pm). The sterile 1,500 ml of antigenic material was stored at -20°C.Antigen preparations from 25 other strains were described earlier (11,14).Antigen preparation for immunization. M. intracellulare cells were grown in two Blake bottles, each containing 200 ml of Long's synthetic medium (5).…”

mentioning

confidence: 99%

Mycobacterium intracellulare Reference Precipitation System

Ridell

Chaparas

Desmettre

et al. 1988

International Journal of Systematic Bacteriology

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Characteristics of a Mycobacterium intracellulare reference precipitation system for immunodiffusion and immunoelectrophoresis analyses are presented. The system was produced by the initiative of the International Working Group on Mycobacterial Taxonomy, and the purpose is to permit comparisons of precipitinogenic patterns of mycobacteria obtained in different laboratories. The reference material, consisting of an antigen preparation and a corresponding antiserum, is available for students of mycobacterial antigens in accordance with guidelines adopted by the Working Group.Immunodiffusion (ID) and immunoelectrophoresis (IE) are used by many investigators for taxonomic analyses and other research studies of mycobacteria (for a review, see reference 4). The knowledge concerning mycobacterial antigens is, however, small and scattered compared with that for many other genera. The need for mycobacterial reference systems that are internationally available for comparison of results from different laboratories has been recognized. Thus, in 1971, such a system was presented for Mycobacterium tuberculosis by the Mycobacterial Antigen Subcommittee of the U.S.-Japan Cooperative Medical Science Program (3). In 1980, Closs et al. (2) introduced a commercially available antiserum against Mycobacterium bovis BCG. These two systems were recently compared (18). The International Working Group on Mycobacterial Taxonomy has found it advantageous to establish reference systems for additional mycobacterial species. Accordingly, an antigen preparation and corresponding antiserum for a serological reference system representing Mycobacterium intracellulare were produced, and the present report describes this system. MATERIALS AND METHODSBacterial strains. The Boone strain of M . intracellulare (TMC 1403) isolated at the Battey State Hospital was used. The strain was obtained from the Trudeau Mycobacterial Culture Collection, currently housed at the American Type Culture Collection, Rockville, Md. In addition, 25 strains of Mycobacterium species and related genera were included for comparison (Table 1).Antigen preparation for serological analyses. M . intracellulare cells were grown on Watson Reid medium (17) as surface growth, Thirty flasks, each containing 1,000 ml of medium, were inoculated from one seed culture. The cultures were incubated at 37°C for 6 weeks, harvested by filtration, and washed three times in sterile distilled water. The washed bacilli were suspended in an equal volume of sterile distilled water and disrupted by sonication six times for 5 min each time in an ice bath, at 150 W, at an output frequency of 23 kHz. An ultrasonic disintegrator (Soniprep * Corresponding author 150; MSE Scientific Instruments, Manor Royal, Crawley , Sussex, England) was used. The extract was centrifuged at 50,000 X g for 60 min at 4"C, and the supernatant was filtered through a Millipore filter (pore size, 0.45 pm). The sterile 1,500 ml of antigenic material was stored at -20°C.Antigen preparations from 25 other strains were described ear...

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Ribosomal ribonucleic acid similarities in the classification of Streptomyces

Gladek

1985

FEMS Microbiology Letters

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1. SUMMARY 2. INTRODUCTION Duplexes were prepared between [X4C]rRNA from Streptomyces albus ISP5313, Streptomyces griseus ISP5236, Streptomyces laoendulae ISP5069 and Streptoverticillium cinnamoneum ISP5005 and DNA from 11 representatives of the genera Streptomyces and Streptoverticillium. The relationships between the organisms were determined by plotting the temperature at which 50% of the duplex was denatured (Tin(e)) against the percentage of rRNA binding (/~g [t4C]rRNA duplexed per 100/~g filter-bound DNA). The Streptomyces strains formed duplexes with high Tin(e) values within narrow ranges in each reference system and thereby occupied distinct regions in the rRNA similarity maps. The Streptoverticillium strains also formed stable duplexes with the Streptornyces reference systems but were distinguished from streptomycetes on the rRNA similarity map based upon the Streptoverticillium cinnamoneum reference system. * To whom correspondence should be sent. The genus Streptomyces contains aerobic, Gram-positive actinomycetes that are highly oxidative and form extensive branching substrate and aerial mycelia, the latter usually beating long chains of spores [11,35]. Streptomycetes have a wall chemotype I, containing LL-diaminopimelic acid and glycine but no characteristic sugar in the peptidogiycan [11,16], and a DNA base composition within the range 69-73 mol % guanine + cytosine [27]. The confused state of streptomycete systematics has impeded the objective choice of strains for detailed chemosystematic and genetical studies, but a recent numerical phenetic survey [35] provided a baseline for the classification of Streptomyces and related taxa. In this study, most of the 394 Streptornyces type cultures were recovered in a large cluster group (A) which contained 19 major, 40 minor and 18 single member clusters. However, several streptomycete species fell outside the Streptomyces cluster group. These included the Streptomyces lavendulae cluster which fell into the same cluster group (F) as Streptoverticilliurn strains.DNA:ribosomal (r)RNA pairing studies provide a convenient way of determining relationships

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Serotaxonomical Analyses of Some Streptomyces and Related Organisms

Cited by 12 publications

References 21 publications

Enzyme-linked Immunosorbent Assay (ELISA) as a Means of Taxonomic Analysis of Streptomyces and Related Organisms

Enzyme-linked Immunosorbent Assay (ELISA) as a Means of Taxonomic Analysis of Streptomyces and Related Organisms

Mycobacterium intracellulare Reference Precipitation System

Ribosomal ribonucleic acid similarities in the classification of Streptomyces

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