Seromucoid (mucoprotein) determinations in experimental animals

An Improved Method for the Determination of Orthophosphate Suitable for Assay of Adenosine Triphosphatase Activity.

“…It was found that myosin precipitated completely with 0.6M CIO4-at pH 1.85, but not at higher pH's (Section III), and Pi was quantitatively recovered in the supernatant (Figure 6). The CIO4concentration used was suggested by the level of HC104 effective for precipitation of serum proteins (26,27). The effect of pH on precipitation of proteins by anions such as C104can be attributed to a requirement for protonation of carboxylate groups.…”