Serological tests and polymerase chain reaction for detection of Toxoplasma gondii infection in women attending for premarital examination

bakre, Hawre Mustafa

doi:10.15218/zjms.2016.0047

Cited by 5 publications

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“…One-way ANOVA and chi-square tests were performed for statistical analyses. The independent t-tests and chi-square tests were performed to determine the association between toxoplasma and reproductive hormones and infertility [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35].…”

Section: Methods and Patientsmentioning

confidence: 99%

Toxoplasmosis Seropositivity and Male Sex Hormones

RiadhHussien¹

2018

AIBM

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Background: Toxoplasmosis is a cosmopolitan disease with acute and chronic infections, caused by the obligate intracellular protozoan parasite Toxoplasma gondii that can infect a variety of cells in almost all warm-blooded animals including humans. The study aimed to determine the seroprevalence of T. gondii infection among males in Duhok city using ELISA (IgG and IgM).The relationship between toxoplasmosis and reproductive hormones including testosterone, free testosterone, and Follicle Stimulating Hormone (FSH) levels and its association with male sterility were also investigated.

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Section: Methods and Patientsmentioning

confidence: 99%

Toxoplasmosis Seropositivity and Male Sex Hormones

RiadhHussien¹

2018

AIBM

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“…An increasing prevalence of toxoplasmosis in humans and animals has been drawn the attention of the researchers and workers to address toxoplasmosis as serious public health problem and many studies have been conducted in different geographical regions of the world and Yemen (Nimri et al, 2004;Sharif et al, 2006;Hamzavi et al, 2007;Saleh et al, 2010;Shahiduzzaman et al, 2011;Subasinghe et al, 2011;Tasawar et al, 2011;Gebremedhin et al, 2013;El-Madawy and Metawea, 2013;Kadle, 2014;Saif et al, 2014;Bakre, 2016;Tonouhewa et al, 2017;Luo et al, 2017;Gharavi et al, 2018). However, the information on toxoplasmosis of human and food animals are limited at Dhamar governorate, Yemen.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological Study on Toxoplasmosis of Human and Animals at Dhamar Governorate, Yemen

Shaibani¹,

Mahdi²,

AlShwkani³

2018

Int.J.Curr.Microbiol.App.Sci

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This cross sectional study was carried out in Dhamar governorate from December 2015 to November, 2016 with main objective to determine the seroprevalence of toxoplasmosis in humans and animals. A total of 323 individuals and 323 animals (241 sheep, 69 cattle and 13 goats) from different districts of Dhamar governorate were selected and tested using ELISA and PCR techniques for detection antibodies against Toxoplasma gondii in human and animals. The results revealed that, the overall seroprevalence of toxoplasmosis in human was 20.43%. The seroprevalence rates of IgG, IgM and both IgG +IgM were 13.62%, 4.33% and 2.48% respectively. The higher rate of infection was recorded (29.50 %) in age group of 11-20years old; whereas, the lower rate (11.11%) was in age group of less than 10 years old. Females were more susceptible to infection (23.19 %) compared to males (2.02 %). The higher seroprevalence rate was recorded in summer (28.41%); whereas, the lower rate in spring (13.15%). The higher prevalence of rate was recorded in Utoma district (32.00%); whereas, lower rate in Alhada district (3.85%). Statistically, significant differences (P<0.05) were observed between seroprevalence rate and age group, sex; while none with season and area factors. In animals, the results of ELIZA revealed that, the overall seroprevalence of toxoplasmosis was 23.84 percent, the seroprevalence of IgG, IgM and both IgG +IgM were 15.5%, 1.5% and 6.8% respectively. Out of 241 sheep, 69 cattle and 13 goats tested, 21.16%, 31.88%, 30.77% respectively were seropositive for toxoplasmosis. The infection rate of toxoplasmosis was high in age group of 5 years old and above (33.33%); whereas, low (21.55%) in age group of two years. The seroprevalence rate in males was (28.28%) higher compared to females (21.88 %). The higher seroprevalence rate was recorded in spring season (33.33%); whereas, the lower rate in winter (18.02.67%). The higher of seroprevalence was recorded in Utoma (35.71%); whereas, the lower rate in Mifa`a ans (10.715%) and none in Jabal asharq. Statistically, significant differences (P<0.05) were observed between seroprevalence rate and animal species, season, area factors; while, none with other factors studied. In the present study, all ELISA seropositive samples of human and animals were retested by PCR as confirmatory tool. Results of PCR revealed that, out of 66 and 77 samples of human and animals tested 30 (45.45%) and 18 (23.38%) were found positive respectively for toxoplasmosis. The results of the present study revealed that toxoplasmosis is prevalent in human and animals in study area. Combinations of serological and molecular techniques are recommended for accurate diagnosis of Toxoplasmosis.

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“…Comparably, our finding was similar to that observed by previous studies, carried out in Erbil. Bakre [20] reported that sero-prevalence rate of toxoplasmosis was 8 (5.3%) and 23 (15.3%) for IgM and IgG, respectively among women who attended Mammon Dabax Health Center in Erbil over the period from January to September 2015. While Hamad [21] observed 9.13% and 37.5% positive cases for IgM and IgG, respectively.…”

Section: Resultsmentioning

confidence: 99%

Sero-prevalence of toxoplasmosis and Role of Cathepsin L-like and Cathepsin B-like Genes as Risk Factors for Abnormal Pregnancy Outcome

omer

Alsakee

2020

MJS

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Background and objectives: Toxoplasma gondii (T. gondii) is a ubiquitous apicomplexan parasite. As an obligate intracellular parasite, T. gondii must invade host cells to survive and replicate. Five cathepsin proteases are encoded in the genome of T. gondii, cathepsin L like protein, cathepsin B like protein, and three cathepsin C like proteins. The present study was aimed to investigate the prevalence of toxoplasmosis among women in Erbil, and to study the role of cathepsin B and cathepsin L genes in the pathogenesis of toxoplasmosis as well as their role as risk factors for abnormal pregnancy outcome. Methods: This is a cross sectional study was carried out in Erbil from October 2018 to March 2019. A total of 230 women at their reproductive age and who attended Maternity Teaching Hospital and Nazdar Bamarni primary Health Center were enrolled. Anti- toxoplasma IgG and IgM antibodies were detected by cobas 6000. Toxoplasma cathepsin B and cathepsin L – like genes were selected to be targets in PCR. Results: Anti-toxoplasma IgG and IgM were seropositive in 105 (45.7%) and 18 (7.8%) women, respectively. Of those women who were seropositive for toxoplasmosis, only 15(6.5%) of them were carrying both anti-toxoplasma IgG and IgM. No significant association of toxoplasmosis and educational level, socioeconomic level, age, history of abortion, abnormal baby birth weight were observed. PCR targeting cathepsin L was more sensitive to be used in the diagnosis of toxoplasmosis. Conclusion: Sero-prevalence of toxoplasmosis is relatively high in Erbil and cathepsin L gene is an efficient target for PCR and could be used as risk factor for abnormal pregnancy outcome.

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Serological tests and polymerase chain reaction for detection of Toxoplasma gondii infection in women attending for premarital examination

Cited by 5 publications

References 0 publications

Toxoplasmosis Seropositivity and Male Sex Hormones

Toxoplasmosis Seropositivity and Male Sex Hormones

Epidemiological Study on Toxoplasmosis of Human and Animals at Dhamar Governorate, Yemen

Sero-prevalence of toxoplasmosis and Role of Cathepsin L-like and Cathepsin B-like Genes as Risk Factors for Abnormal Pregnancy Outcome

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