Serological survey of Toxoplasma gondii and Besnoitia besnoiti in a wildlife conservation area in southern Portugal

Waap, Helga; Nunes, Telmo; Vaz, Yolanda; Leitão, Alexandre

doi:10.1016/j.vprsr.2016.05.003

Cited by 14 publications

(15 citation statements)

References 53 publications

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“…Little is known about T. gondii circulation in the wild rabbits of Portugal. The available data are focused only in the southeast of the country, with a T. gondii seroprevalence of 2.8% (n = 36) [20]. In other countries, most of the studies found on T. gondii in European wild rabbit are also focused on serological analysis, with seroprevalence levels varying from 3.3% in Scotland [19] to 21.0% in Norway and Sweden [17].…”

Section: Discussionmentioning

confidence: 99%

“…The current T. gondii infection levels in European wild rabbit in Portugal are not really known. To the best of our knowledge, just one recent study in wild rabbits has been reported to date in Portugal, describing a T. gondii seroprevalence of 2.8% [20]. The purpose of the present work was to detect T. gondii DNA in European wild rabbit from central Portugal, as well as the potential implications for public health.…”

Section: Introductionmentioning

confidence: 94%

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PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

et al. 2020

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Wildlife plays an important role in the epidemiological cycle of Toxoplasma gondii. The European wild rabbit (Oryctolagus cuniculus) can be a source of infection to wild and domestic hosts, including human beings. Additionally, as an herbivorous animal, the European wild rabbit may also be a sentinel of environmental contamination with T. gondii and, consequently, an indicator of the potential transmission of this parasite. The purpose of the present work was to detect T. gondii DNA in European wild rabbit from central Portugal, as well as the possible implications for public health. Heart and diaphragm samples were obtained from 28 rabbits hunted in central Portugal. Nested PCR separately amplified the 5′ and 3′ ends of the surface antigen 2 (SAG2) gene. T. gondii DNA was detected in 19 out of the 28 sampled animals, resulting in a prevalence of 67.9%. These results show that T. gondii infection occurs in European wild rabbit and therefore may pose a potential risk for humans if consumed as raw or undercooked meat. Measures should be taken in order to prevent infection by this zoonotic parasite and for the conservation of wildlife. To the best of our knowledge, this is the first study performed by means of PCR on T. gondii in European wild rabbit meat samples.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

et al. 2020

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“…1988), canine parvovirus (Duarte et al 2012) and Toxoplasma gondii (Jakubek et al . 2012; Waap et al 2016).…”

Section: Methodsmentioning

confidence: 99%

“…Lung tissue samples were collected during necropsy and processed as described by Ferroglio et al (2000) to prepare lung extracts for serological analysis. Lung extracts were shown to be suitable for the screening of specific antibodies against several pathogens in wildlife, including Encephalitozoon (Waller et al 1980), Francisella tularensis (Mörner et al 1988), canine parvovirus (Duarte et al 2012) and Toxoplasma gondii (Jakubek et al 2012;Waap et al 2016).…”

Section: Analysis Of Anti-n Caninum-specific Igg Antibodies In Wildlmentioning

confidence: 99%

Serological study of Neospora caninum in dogs and wildlife in a nature conservation area in southern Portugal

et al. 2017

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SUMMARYA serological survey was performed to evaluate the presence of specific antibodies againstNeospora caninumin dogs and native animals in a wildlife conservation area in southern Portugal. The study involved 463 animals, including dogs (n = 286), European rabbits (Oryctolagus cuniculus;n = 32), Egyptian mongoose (Herpestes ichneumon;n = 34), wild boars (Sus scrofa;n = 26), foxes (Vulpes vulpes,n = 25), common genets (Genetta genetta;n = 17), red deer (Cervus elaphus;n = 14), wildcats (Felis silvestris;n = 6), four mustelid species (n = 17) and rodents (n = 6). Samples from dogs were analysed by Indirect Fluorescent Antibody Test (IFAT). Samples from wild animals were screened by the modified agglutination test and positive and doubtful results were confirmed by IFAT. The seroprevalence ofN. caninumin dogs was 32·5% [95% confidence interval (95% CI) 27·3–38·1]. Among wild animals, exposure toN. caninumwas confirmed only in foxes (12%, 95% CI 4·2–30) and rabbits (25%, 95% CI 13·3–42·1). This is the first evidence of natural exposure toN. caninumin foxes and rabbits in Portugal, and our results suggest that rabbits may play a role as reservoirs of infection to dogs, foxes and other wildlife carnivores. The relevance of this finding in the sylvatic cycle ofN. caninumneeds further studies, since infection may affect wildlife species and cattle grazing in the same areas.

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“…Positive sera were titrated at dilutions: 1:25, 1:50, 1:100 and 1:500. MAT has been extensively used for the serological diagnosis of T. gondii in domestic and wild ruminant species (Almería et al, 2018;Aubert et al, 2010;Gamarra et al, 2008;Heddergott et al, 2018b;Heddergott et al, 2018a;Jiménez-Martín et al, 2020;Waap et al, 2016). A comparison of seropositivity and isolation of viable T. gondii in white tailed deer (Odocoileus virginianus) in the USA attests the efficiency of MAT for the detection of T. gondii antibodies in deer (Dubey et al, 2020).…”

Section: Testing For T Gondii Antibodiesmentioning

confidence: 99%

Seroepidemiology of Toxoplasma gondii in wild ruminants in Spain

Castro‐Scholten

Cano‐Terriza

Jiménez‐Ruiz

et al. 2021

Zoonoses and Public Health

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Serological survey of Toxoplasma gondii and Besnoitia besnoiti in a wildlife conservation area in southern Portugal

Cited by 14 publications

References 53 publications

PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

Serological study of Neospora caninum in dogs and wildlife in a nature conservation area in southern Portugal

Seroepidemiology of Toxoplasma gondii in wild ruminants in Spain

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