Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil

Batista, Fernanda Gonçalves; Silva, Daniella Matos da; Green, Kerriel Thandile; Tezza, Louise Boulsfield de Lorenzi; Vasconcelos, Sâmara P; Carvalho, Suelen Graziele Soares de; Silveira, Iara; Moraes‐Filho, Jonas; Labruna, Marcelo Bahia; Fortes, Filomeno; Molento, Marcelo Beltrão

doi:10.1590/s1984-29612010000400003

Cited by 15 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Caratinga in the state of Minas Gerais, Cardoso et al (2006) [ 19 ] found reactive serology in 3 of 18 (17%) horses’ samples and detected DNA of pathogenic Rickettsia in arthropod vectors in a region with no reported cases of BSF in the last 12 years. In Paraná state, Batista et al (2010) [ 20 ] also found horses and dogs positive for R . rickettsii in areas with no reported human case.…”

Section: Discussionmentioning

confidence: 99%

High Seroprevalence for Rickettsia rickettsii in Equines Suggests Risk of Human Infection in Silent Areas for the Brazilian Spotted Fever

Souza¹,

Camargo

Pintér³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Equines play a role in the epidemiology of Brazilian spotted fever (BSF) since they are a primary host for the tick Amblyomma sculptum. We studied the seroprevalence for three species of Rickettsia in equines in four endemic (with human cases) and in four non-endemic areas (no human cases) in the Piracicaba River Basin, São Paulo, Brazil. A serological survey of 504 equines was performed: around 63 animals were sampled in each area and tested through indirect immunofluorescence assay for R. rickettsii, R. parkeri, and R. bellii in 2012–2013. Blood samples were seropositive for 183 equines (36.3%) in which 73 (39.9%) were from non-endemic areas. In the studied sites equines were highly exposed to Rickettsia infection ranging from 6.1% to 54.7%, with Geometric Mean Titers greater in endemic area (p = 0.012). Results suggest that Rickettsia may be more widespread than the surveillance of BSF has detected. These results highlight the need to include data on the seroprevalence of sentinel animals to improve human diagnoses and surveillance in areas with no reported human cases.

show abstract

Section: Discussionmentioning

confidence: 99%

High Seroprevalence for Rickettsia rickettsii in Equines Suggests Risk of Human Infection in Silent Areas for the Brazilian Spotted Fever

Souza¹,

Camargo

Pintér³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…These other studies indicated positive rates ranging from 5.5 to 38.5% in horses, from 0 to 19.1% in dogs and from 4.7 to 21.0% in humans (BATISTA et al, 2010;FREITAS et al, 2010;OTOMURA et al, 2010;TAMEKUNI et al, 2010;TAMEKUNI et al, 2011;TOLEDO et al, 2011), according to the geographic area. The low prevalence found in this study suggests that dogs and horses are exposed to a small vector population infected by Rickettsiae.…”

Section: Discussionmentioning

confidence: 86%

Probability of occurrence of the Brazilian spotted fever in northeast of Paraná state, Brazil

Otomura

Truppel

Filho

et al. 2016

Rev. Bras. Parasitol. Vet.

Self Cite

View full text Add to dashboard Cite

Brazilian spotted fever (BSF) is a fatal zoonosis because of the difficulties in its early diagnosis and treatment. Occurrences of BSF in the northeast of the state of Paraná prompted investigation of areas at risk of this rickettsiosis in the municipalities of Japira, Jaboti, Pinhalão and Tomazina. To determine the areas at risk, 592 serum samples from dogs and 230 from equids were analyzed by means of the indirect immunofluorescence assay (IFA) for Rickettsia rickettsii and R. parkeri . In addition, risk probability maps were drawn up using the kriging indicator technique. Among the samples tested, 5.3% (43/822) indicated presence of antibodies reactive to at least one of the two Rickettsia species tested: 7.8% of the equids (18/230) and 4.2% of the dogs (25/592) were positive. Geostatistical analysis showed that the average seropositivity rate was 5 to 6%. Although the average seropositivity rates observed among these dogs and equids were lower than those reported from endemic areas of Brazil, the biotic components (etiological agent, vector and reservoirs) and environmental aspects of BSF epidemiology were present in these municipalities.Keywords: Rickettsia rickettsii, Rickettsia parkeri, risk map, serology, geostatistical analysis. ResumoA febre maculosa brasileira (FMB) é uma zoonose fatal devido às dificuldades para diagnosticá-la e tratá-la precocemente. A ocorrência de casos de FMB no Estado do Paraná suscitou a investigação de áreas de risco desta rickettsiose nos municípios de Japira, Jaboti, Pinhalão e Tomazina, na mesorregião norte pioneiro do Paraná. Para determinar as áreas de risco foram analisadas amostras de soro de 592 cães e 230 equídeos submetidos à reação de imunofluorescência indireta para Rickettsia rickettsii e R. parkeri. Além disto, foram construídos mapas de probabilidade de risco pela técnica de krigagem indicatriz. Das amostras testadas 5,3% (43/822) continham anticorpos para pelo menos uma das duas rickettsias testadas. Os equídeos apresentaram uma positividade de 7,8% (18/230) e os cães de 4,2% (25/592). A análise geoestatística mostrou que a soropositividade média é de 5 a 6%. Embora as soropositividade médias de cães e equídeos constatadas tenham sido menores do que as relatadas em áreas endêmicas do território brasileiro, os componentes bióticos (agente etiológico, vetor e reservatórios) e ambientais da epidemiologia da FMB se fazem presentes nos municípios referidos.Palavras-chave: Rickettsia rickettsii, Rickettsia parkeri, mapa de risco, sorologia, análise geoestatística.

show abstract

“…in places where the incriminated vectors of the disease were not found, contrasting with places where there were positive arthropods in real-time PCR for Rickettsia spp. and no seropositive animals (Batista et al, 2010;Medeiros et al, 2013;Toledo et al, 2009).…”

Section: Discussionmentioning

confidence: 94%

“…Medeiros et al (2013) demonstrated the occurrence of cross-reaction between different species of Rickettsia in horses. Positive serological responses in horses in areas with no tick exposure or negative serology in equines with tick exposure were described in different areas in Brazil (Batista et al, 2010;Pacheco et al, 2011). Freitas et al (2010) collected blood samples from 75 carter horses in São José dos Pinhais and found 9.33% positivity in the animals, with titers between 64 and 1,024 in the indirect immunofluorescence test for Rickettsia spp.…”

Section: Discussionmentioning

confidence: 99%

Serological survey of Rickettsia in equids from Vale do Paraíba, São Paulo, Brazil, and their tick identification and molecular investigation of Rickettsia

Budweg¹,

Sousa²,

Carvalho³

et al. 2019

Braz. J. Vet. Res. Anim. Sci.

Self Cite

View full text Add to dashboard Cite

Brazilian spotted fever is a serious and lethal illness for humans and is caused by the Rickettsia rickettsii bacteria. In the state of São Paulo/SP (Brazil), the etiological agent of this disease is transmitted by the Amblyomma sculptum tick. It was already shown that horses infected with this bacteria produce a strong immune response and could be important sentinels for the detection of the disease in a proper region. The present investigation performed a serological survey in horses from five farms of Vale do Paraíba, São Paulo state, Brazil, searching for antibodies against, Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommatis, Rickettsia rhipicephali, and Rickettsia bellii. In each farm, ticks were also collected that were taxonomically identified and examined by real-time PCR for Rickettsia spp DNA. Blood samples were collected from 206 horses, and 334 ticks were picked up from these animals from January to December 2017. Eighty ticks wereA. sculptum and 254 Dermacentor nitens. Of the blood samples, 7.3% seroconverted to Rickettsia spp. Of these, 0.97% had a positive serological response to R. bellii. None of the 80 A. sculptum ticks were positive through real-time PCR for Rickettsia spp. Although there was no detection of ticks infected by Rickettsia spp in five farms of Paraíba Valley, the horses presented serological positive reactions against this agent. Thus, further large studies should be conducted in the area targeting hosts and vectors to generate data for control measures of the transmission of Brazilian spotted fever.

show abstract

Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil

Cited by 15 publications

References 27 publications

High Seroprevalence for Rickettsia rickettsii in Equines Suggests Risk of Human Infection in Silent Areas for the Brazilian Spotted Fever

High Seroprevalence for Rickettsia rickettsii in Equines Suggests Risk of Human Infection in Silent Areas for the Brazilian Spotted Fever

Probability of occurrence of the Brazilian spotted fever in northeast of Paraná state, Brazil

Serological survey of Rickettsia in equids from Vale do Paraíba, São Paulo, Brazil, and their tick identification and molecular investigation of Rickettsia

Contact Info

Product

Resources

About